Abstract:
:BACKGROUND Senescence is a natural barrier for the body to resist the malignant transformation of its own cells. This work investigated the senescence characteristics of cancer cells in vitro. MATERIAL AND METHODS Human cervical cancer HeLa cells were treated with different concentrations of doxorubicin for 3 days, with or without subsequent extended culture in drug-free medium for 6 days. Senescent cell ratios between these 2 culture schemes were calculated. Expression of 2 senescence-associated secretory factors, IL-6 and IL-8, were detected by RT-PCR and ELISA. Doxorubicin treatment induced epithelial-mesenchymal transition in cancer cells. The proportions of senescent cells in epithelial-like and mesenchymal-like sub-groups were calculated. Doxorubicin-treated HeLa cells were stained with Vimentin antibody and sorted by flow cytometry. Senescent cell marker p16ᴵᴺᴷ⁴ᵃ and IL-8 expression in Vimentin-high and Vimentin-low cells were detected by Western blot. RESULTS We found that less than 1% of HeLa cells showed senescence phenotype after treatment with doxorubicin for 3 days. However, the proportion of senescent cells was significantly increased when the doxorubicin-treated cells were subsequently cultured in drug-free medium for another 6d. RT-PCR and ELISA results showed that this prolonged culture method could further improve the expression of IL-6 and IL-8. We also found that the senescent cells were mainly epithelial-like type and few presented mesenchymal-like shape. p16ᴵᴺᴷ⁴ᵃ and IL-8 expression were decreased in cell fraction with higher Vimentin expression. CONCLUSIONS Our results suggested the existence of time delay effect in doxorubicin-induced senescence of HeLa cells, and epithelial- mesenchymal transition may resist doxorubicin-induced cell senescence.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Hu X,Zhang Hdoi
10.12659/MSM.914295subject
Has Abstractpub_date
2019-05-16 00:00:00pages
3617-3623eissn
1234-1010issn
1643-3750pii
914295journal_volume
25pub_type
杂志文章abstract::BACKGROUND Phenylketonuria (PKU) is an inborn error of metabolism caused by mutations in the phenylalanine hydroxylase (PAH) gene. When untreated, PKU leads to a significant intellectual deficiency. Although early initiation of dietary therapy allows normal cognitive development, low adherence to treatment may result ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.909146
更新日期:2018-10-30 00:00:00
abstract::BACKGROUND The prevalence of peripheral arterial disease (PAD) is increasing worldwide. Currently, there is no effective treatment for PAD. Curcumin is an ingredient of turmeric that has antioxidant, anti-inflammation, and anticancer properties. In the present study we investigated the potential effect of curcumin in ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.896217
更新日期:2016-06-15 00:00:00
abstract:BACKGROUND:The aim of this study was to investigate the protective effects of L-glutamine (GLN) against liver and kidney injury caused by acute toxicity of deltamethrin (DLM). MATERIAL AND METHODS:Thirty-two rats were indiscriminately separated into 4 groups with 8 rats each: control group (distilled water; 10 ml/kg, ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.893180
更新日期:2015-04-19 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.919266
更新日期:2019-12-11 00:00:00
abstract:BACKGROUND:Genotypic analysis has been performed in Helicobacter pylori strains isolated before and after antibiotic therapy from patients for whom no eradication of the microbes could be achieved. MATERIAL/METHODS:The Helicobacter pylori strains were isolated from 10 patients with duodenal ulcer, who were treated by ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:Oral breathing causes many changes in the facial anatomical structures in adult patients. In this study we aimed to determine the effects of long-term oral breathing (>5 years) on the maxillary sinus volumes among adult male patients. MATERIAL/METHODS:We accessed medical records of 586 patients who had unde...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.891371
更新日期:2015-01-02 00:00:00
abstract:BACKGROUND:Prognosis in patients with gastric cancer is determined by the tumor itself, as well as certain patient-related factors. MATERIAL/METHODS:In this study, 138 patients with high-grade gastric adenocarcinoma who were admitted to our hospital between September 1999 and April 2002 were retrospectively evaluated ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::BACKGROUND Repetition disorder can be used as an important criterion for aphasia classification, and damaged arcuate fasciculus in the dominate hemisphere has been reported to be closely related to repetition disorder, but the underlying neurological mechanism remains unclear. MATERIAL AND METHODS Fifteen stroke patie...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.928702
更新日期:2020-12-05 00:00:00
abstract::The stress hormones plasma concentration after intravenous anesthetics (thiopentone, propofol, midazolam) administration in patients who underwent non-toxic struma operation was estimated. The goal of the study was to answer what stage of the general anesthesia and the surgery is the most dangerous for the cardiovascu...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::BACKGROUND Clogging of the suction tip frequently occurs during orthopedic surgery. We developed a novel anti-clog suction tip using 3D printing technology to improve orthopedic surgery efficiency. MATERIAL AND METHODS We studied the root causes of obstructions in suction tips currently employed in orthopedic surgery ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.909900
更新日期:2018-09-25 00:00:00
abstract:BACKGROUND:Growing resistance to antibiotics among both community-acquired and hospital pathogens is making the treatment of most infections increasingly difficult. Therefore, the aim of our multi-center study was to determine the antimicrobial susceptibility of the most frequent aerobic microorganisms isolated from ch...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::BACKGROUND The aim of this study was to investigate the association between body mass index (BMI) and brachial-ankle pulse wave velocity (baPWV) in hypertensive males. MATERIAL AND METHODS Altogether, 14 866 male hypertensive participants were included in the analysis. Participants were divided into 3 groups: low BMI ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.914881
更新日期:2019-07-15 00:00:00
abstract::BACKGROUND The factors associated with osteoporosis are poorly understood in the Chinese population. This study aimed to examine the factors associated with osteoporosis and with fractures in a Chinese elderly population. MATERIAL AND METHODS This was a cross-sectional study of elderly people living in Tianjin between...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.914182
更新日期:2019-07-27 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.914409
更新日期:2019-07-06 00:00:00
abstract:BACKGROUND:The aim was to determine the effectiveness of continuous aminophylline infusion on refractory bronchospasm in long-term mechanically ventilated neonates. CASE REPORT:Presented are seven newborns with gestational ages from 24 to 38 weeks and mean age at treatment of 29.7 days. All were mechanically ventilate...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND:The main objective of this study was to create an experimental model of idiopathic scoliosis (i.s.), and to assess the effect of Lateral Electrical Surface Stimulation (LESS) on the organism both intra vitam and post mortem. The experiment made it possible to determine the extent to which LESS affects overal...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::BACKGROUND Levetiracetam (LEV) is an antiepileptic drug that promotes recovery of neurological function by alleviating inflammatory reactions. However, it is not known whether it can improve secondary brain injury after intracerebral hemorrhage (ICH). The aim of this study was to determine whether LEV can reduce early...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.922741
更新日期:2020-04-14 00:00:00
abstract::BACKGROUND The objective of the present study was to identify the association between miR-15a-5p and CDKN2B, and their roles in regulating the development of abdominal aortic aneurysm (AAA). MATERIAL AND METHODS We searched the miRNA database online (www.mirdb.org) and used a luciferase reporter assay system to study ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.898233
更新日期:2017-02-18 00:00:00
abstract::In December 2019, an outbreak of coronavirus infection emerged in Wuhan, Hubei Province of China, which is now named Coronavirus Disease 2019 (COVID-19). The outbreak spread rapidly within mainland China and globally. This paper reviews the different imaging modalities used in the diagnosis and treatment process of CO...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.924582
更新日期:2020-07-12 00:00:00
abstract::BACKGROUND Simazine is a triazine herbicide used worldwide in both agricultural and non-agricultural fields that is frequently detected in surface water and groundwater. Due to its widespread use, an increasing amount of research has focused on the potentially serious environmental and health risks. MATERIAL AND METHO...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.896460
更新日期:2016-08-11 00:00:00
abstract::BACKGROUND This study compared the efficacy and safety of 3 different anesthesia techniques used in total hip arthroplasty (THA). MATERIAL AND METHODS We allocated 198 patients preparing to undertake THA into 3 groups: general anesthesia group (GA group, n=66), caudal epidural anesthesia group (CEA group, n=66), and s...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
doi:10.12659/msm.902768
更新日期:2017-08-02 00:00:00
abstract::The Na⁺/Ca²⁺ exchanger (NCX) is an important electrogenic transporter in maintaining Na⁺ and Ca²⁺ homeostasis in a variety of mammalian organs, and is involved in the physiological and pathophysiological regulation of Ca²⁺ concentration in the myocardium. It can affect cardial structure, electrophysiology and contract...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:10.12659/msm.883533
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND:Plasma levels of L-arginine have previously been evaluated in several studies to reveal either down- or up-regulation of the L-arginine/NO pathway in chronic uremia. MATERIAL/METHODS:We studied L-arginine plasma levels along with intracellular neutrophil concentration and NO release by peripheral blood neut...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract:BACKGROUND:Two hundred fifty one men and women participated in a study of the prediction of fear of heights, snakes, and public speaking by providing retrospective accounts of multimodal classical conditioning events involving those stimuli. The fears selected for study represent those believed by some to be innate (i....
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::BACKGROUND T follicular helper (Tfh) cells are a subgroup of activated CD4+ T cells in the germinal centers of secondary lymphoid organs, they play critical roles in the development of many chronic autoimmune inflammatory diseases. The aim of this study was to investigate whether circulating Tfh cells contribute to th...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.914868
更新日期:2019-04-25 00:00:00
abstract:BACKGROUND:Giardiasis is caused by the intestinal protozoan parasite Giardia duodenalis (synonyms: G. lamblia, G. intestinalis), which is one of the most frequent parasites that infect Turkish children. However, molecular characterization of G. duodenalis in Turkey is relatively scarce. The present work aimed at genoty...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.892318
更新日期:2015-02-18 00:00:00
abstract::BACKGROUND Pancreatic cancer is a major cause of mortality worldwide. Inefficient drugs, their adverse effects, and the development of drug resistance make it difficult to curb the growing incidence of pancreatic cancer. Against this backdrop, the development new drug regimens with no or negligible adverse effects is ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.914244
更新日期:2019-04-21 00:00:00
abstract:BACKGROUND:Retinoblastoma (Rb) is the most common primary malignant intraocular tumour in childhood. The "two hit" theory, formulated by Knudson in 1971 to explain the variegated clinical expression of the disease, led to the discovery of the so called tumour suppressor genes and the identification of the Rb1 as the pr...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:
更新日期:2008-12-01 00:00:00
abstract::BACKGROUND The aim of this study was to analyze the differences in vaginal microecological factors and genital tract infections among pregnant women of different ages. MATERIAL AND METHODS This study included 751 pregnant women from West China Second University Hospital, Sichuan University, China, from January 2015 to...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.909051
更新日期:2018-08-01 00:00:00
abstract::BACKGROUND This study aimed to undertake an analysis of ten years of real-world evidence (RWE) on overall survival (OS) following treatment of advanced gastrointestinal stromal tumor (GIST) with imatinib, sunitinib, and sorafenib using data from the Polish National Health Fund. MATERIAL AND METHODS Data from the Polis...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.914517
更新日期:2019-05-23 00:00:00