Long Stress Induced Non-Coding Transcripts 5 (LSINCT5) Promotes Hepatocellular Carcinoma Progression Through Interaction with High-Mobility Group AT-hook 2 and MiR-4516.

Abstract:

:BACKGROUND Long non-coding RNAs (lncRNAs) have been implicated in various human cancer types. However, the underlying mechanisms involved in hepatocellular carcinoma (HCC) progression remain poorly understood. MATERIAL AND METHODS In this study, lncRNA array was used to identify HCC related lncRNAs. RNA immunoprecipitation (RIP) followed mass spectrometry was used to explore lncRNA binding proteins. Western blot, quantitative PCR, tumor sphere formation, migration and viability assay were performed to evaluate the oncogenic role of lncRNAs. RESULTS We identified a novel lncRNA named long stress induced non-coding transcripts 5 (LSINCT5) which facilitates HCC progression. LSINCT5 was significantly upregulated in both HCC specimens and cell lines and correlates with poor survival. In vitro experiments showed that LSINCT5 promoted migration and viability of HepG2 and Huh7 cells. The in vivo xenograft mouse model also confirmed an oncogenic role for LSINCT5. RIP in combination with mass spectrometry identified HMGA2 as the LSINCT5 binding partner. LSINCT5 could bind to HMGA2 and decrease proteasome-mediated HMGA2 degradation leading to EMT activation. LSINCT5 also served as a competing endogenous RNA (ceRNA) for miR-4516, resulting in increased STAT3/BclxL expression and attenuated apoptosis. CONCLUSIONS Our data have collectively established a lncRNA LSINCT5 mediated process during HCC carcinogenesis and might have provided novel insight into therapeutic targeting.

journal_name

Med Sci Monit

authors

Li O,Li Z,Tang Q,Li Y,Yuan S,Shen Y,Zhang Z,Li N,Chu K,Lei G

doi

10.12659/MSM.911179

subject

Has Abstract

pub_date

2018-11-25 00:00:00

pages

8510-8523

eissn

1234-1010

issn

1643-3750

pii

911179

journal_volume

24

pub_type

杂志文章
  • Increased mean platelet volume in chronic hepatitis B patients with inactive disease.

    abstract:BACKGROUND:Hepatitis B virus (HBV) infection is a major public health problem worldwide, responsible for considerable morbidity and mortality from chronic liver disease. Conflicting findings on the possible association between HBV surface antigen (HBsAg) positivity, indicating inactive HBsAg carrier status, and atheros...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Turhan O,Coban E,Inan D,Yalcin AN

    更新日期:2010-04-01 00:00:00

  • Mitochondrial changes induced by trimetazidine in the myocardium.

    abstract:BACKGROUND:The aim was to assess the effect of trimetazidine (TMZ) on mitochondrial alterations induced in a canine model of brief, repeated episodes of ischemia. MATERIAL/METHODS:Twelve crossbred dogs were analyzed, after double-blind randomization, to a 7-day treatment with either TMZ or placebo. Twenty brief, compl...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Morillas Blasco PJ,Hernándiz Martinez A,Azorín Villena I,Portolés Sanz M,Pallarés Carratala V,Martinez VB,Capdevilla Carbonell C,Aguilar Llopis A,Cosín Aguilar J

    更新日期:2005-06-01 00:00:00

  • Minimally Invasive Unilateral vs. Bilateral Pedicle Screw Fixation and Lumbar Interbody Fusion in Treatment of Multi-Segment Lumbar Degenerative Disorders.

    abstract::BACKGROUND The choice for instrumentation with minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) in treatment of degenerative lumbar disorders (DLD) remains controversial. The goal of this study was to investigate clinical outcomes in consecutive patients with multi-segment DLD treated with unilater...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,收录出版

    doi:10.12659/msm.894890

    authors: Liu X,Li G,Wang J,Zhang H

    更新日期:2015-11-25 00:00:00

  • Identification of Target Genes and Transcription Factors in Mice with LMNA-Related Dilated Cardiomyopathy by Integrated Bioinformatic Analyses.

    abstract::BACKGROUND Dilated cardiomyopathy (DCM), which is characterized by enlarged ventricular dimensions and systolic dysfunction, is the most common type of cardiomyopathy. Mutations in the LMNA gene are reported in approximately 10% of familial DCM cases. However, the mechanism of LMNA mutations in human DCM remains uncle...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.924576

    authors: Zhou H,Tan L,Lu T,Xu K,Li C,Liu Z,Peng H,Shi R,Zhang G

    更新日期:2020-06-14 00:00:00

  • Development of a site sampling form for evaluation of ED overcrowding.

    abstract:BACKGROUND:ED overcrowding is fast becoming a national crisis although no definition exists. The purpose of this study is to develop and pilot a sampling form that accurately reflects the concept of ED Overcrowding. MATERIAL/METHODS:A 26-question site-sampling form was designed based on input from academic physicians ...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Weiss SJ,Arndahl J,Ernst AA,Derlet R,Richards J,Nick TG

    更新日期:2002-08-01 00:00:00

  • Effect of carbogen on tumour oxygenation and 32P-colloid interstitial irradiation response.

    abstract:BACKGROUND:Interstitial irradiation therapy using radionuclides is a slow and continual process in which the effect is exerted gradually, thus improvement of the hypoxic status of the tumor will also take a long time. It has been known that carbogen delivery of 5-15 min increases tumor oxygenation. However, the long-te...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Zhan HW,Liu HB,Bao CK,Ye XJ,Zhang H,He GQ

    更新日期:2010-01-01 00:00:00

  • Substance P Inhibits the Collagen Synthesis of Rat Myocardial Fibroblasts Induced by Ang II.

    abstract::BACKGROUND The aim of this study was to explore the regulating effects of Substance P (SP) on the collagen synthesis of rat myocardial fibroblasts (CFBs) induced by angiotensin II (Ang II) and its potential mechanism. MATERIAL AND METHODS The CFBs of a neonatal SD rat were separately cultured and divided into the cont...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.898454

    authors: Yang Z,Zhang X,Guo N,Li B,Zhao S

    更新日期:2016-12-16 00:00:00

  • Pharmacokinetic Analysis of Propofol Target-Controlled Infusion Models in Chinese Patients with Hepatic Insufficiency.

    abstract::BACKGROUND Effects of liver dysfunction on target-controlled infusion (TCI) with Marsh parameters of propofol remain poorly documented. The purpose of this study was to evaluate the performance of propofol TCI in a cohort of Chinese patients with severe hepatic insufficiency. MATERIAL AND METHODS We assigned 32 patien...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.910103

    authors: Chi X,Pan J,Cai J,Luo G,Li S,Yuan D,Rui J,Chen W,Hei Z

    更新日期:2018-09-30 00:00:00

  • Analysis of the relation between level of asthma control and depression and anxiety.

    abstract:BACKGROUND:Frequent co-existence of bronchial asthma and depression or anxiety is an unquestioned phenomenon. In contrast, little is known about the relationship between the degree of asthma control and the prevalence of depression and anxiety. The aim of this study was to determine the potential relationship between t...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.882524

    authors: Trzcińska H,Przybylski G,Kozłowski B,Derdowski S

    更新日期:2012-03-01 00:00:00

  • Lack of family-based association between common variations in WNK1 and blood pressure level.

    abstract:BACKGROUND:WNK1 (With No-lysine Kinase 1) modulates numerous sodium transport-related ion channels involved in regulation of blood pressure. Several studies have indicated associations between the common variants of the WNK1 gene and hypertension or blood pressure levels. However, little data exists on Asian population...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.890791

    authors: Liu F,Lian Q,Ren J,Ren K,Wang Y,Wang D,Chu C,Wang L,Guo T,Liu E,Mu J,Yuan Z

    更新日期:2014-10-16 00:00:00

  • Effect of Fine Particulate Matter (PM2.5) on Rat Placenta Pathology and Perinatal Outcomes.

    abstract::BACKGROUND Fine particulate matter with aerodynamic diameters smaller than 2.5 μm (PM2.5) has been reported to cause adverse effects on human health. Evidence has shown the association between PM2.5 exposure and adverse perinatal outcomes, and the most common method is epidemiological investigation. We wished to inves...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.897808

    authors: Liu Y,Wang L,Wang F,Li C

    更新日期:2016-09-15 00:00:00

  • The correlation between red blood cell distribution width levels with the severity of obstructive sleep apnea and carotid intima media thickness.

    abstract:BACKGROUND:Obstructive sleep apnea syndrome (OSAS) is characterized by repetitive collapse of the upper airway during sleep. Red blood cell distribution width (RDW) increases platelet activation and has been reported as an independent predictor of adverse outcomes in the general population and is believed to be associa...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.891001

    authors: Gunbatar H,Sertogullarindan B,Ekin S,Akdag S,Arisoy A,Sayhan H

    更新日期:2014-11-07 00:00:00

  • Analysis of risk factors for acute kidney injury after ascending aortic replacement combined with open placement of triple-branched stent graft in type A aortic dissection: a new technique versus the traditional method in a single Chinese center.

    abstract:BACKGROUND:We aimed to analyze the risk factors and prognosis of acute kidney injury (AKI) after aortic arch repair in type A aortic dissection. MATERIAL AND METHODS:We included 155 patients undergoing arch repair surgery for type A aortic dissection from January 2009 to January 2014 in our hospital. Ninety-three pati...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.892492

    authors: Qiu Z,Chen L,Cao H,Zhang G,Xu F,Chen Q

    更新日期:2015-03-04 00:00:00

  • Reduction of Airborne Bacterial Burden in the OR by Installation of Unidirectional Displacement Airflow (UDF) Systems.

    abstract:BACKGROUND:Intraoperative bacterial contamination is a major risk factor for postoperative wound infections. This study investigated the influence of type of ventilation system on intraoperative airborne bacterial burden before and after installation of unidirectional displacement air flow systems. MATERIAL AND METHOD...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.894251

    authors: Fischer S,Thieves M,Hirsch T,Fischer KD,Hubert H,Beppler S,Seipp HM

    更新日期:2015-08-13 00:00:00

  • The Na+/Ca²+ exchanger in cardiac ischemia/reperfusion injury.

    abstract::The Na⁺/Ca²⁺ exchanger (NCX) is an important electrogenic transporter in maintaining Na⁺ and Ca²⁺ homeostasis in a variety of mammalian organs, and is involved in the physiological and pathophysiological regulation of Ca²⁺ concentration in the myocardium. It can affect cardial structure, electrophysiology and contract...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,评审

    doi:10.12659/msm.883533

    authors: Chen S,Li S

    更新日期:2012-11-01 00:00:00

  • Treatment options for benign prostatic hyperplasia in older men.

    abstract:BACKGROUND:Benign prostatic enlargement (BPE) is one of the most common diseases in ageing men; it is associated with bothersome lower urinary tract symptoms that affect the individual's quality of life. This review focuses on the different available options for treating benign prostatic hyperplasia (BPH) in older men ...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,评审

    doi:

    authors: Miano R,De Nunzio C,Asimakopoulos AD,Germani S,Tubaro A

    更新日期:2008-07-01 00:00:00

  • Clinically important factors influencing endothelial function.

    abstract::The endothelium, a continuous cellular monolayer lining the blood vessels, has an enormous range of important homeostatic roles. It serves and participates in highly active metabolic and regulatory functions including control of primary hemostasis, blood coagulation and fibrinolysis, platelet and leukocyte interaction...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,评审

    doi:

    authors: Vapaatalo H,Mervaala E

    更新日期:2001-09-01 00:00:00

  • Extended-spectrum beta-lactamase-producing Enterobacteriaceae strains in community-acquired bacteremia in Southern Israel.

    abstract:BACKGROUND:In recent years, extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have emerged in many hospitals worldwide. The increasing dissemination and long-term carriage of these organisms within the community carry tremendous implications on the empirical therapy of community-acquired infection. ...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Borer A,Gilad J,Menashe G,Peled N,Riesenberg K,Schlaeffer F

    更新日期:2002-01-01 00:00:00

  • A Nomogram to Improve Predictability of Small-Incision Lenticule Extraction Surgery.

    abstract::BACKGROUND This study aimed to determine and validate that use of a nomogram could enhance the predictability of small-incision lenticule extraction (SMILE) surgery. MATERIAL AND METHODS 195 eyes from 98 patients were enrolled in group 1, and 46 eyes from 26 patients in group 2. Uncorrected and corrected distance visu...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.904598

    authors: Liang G,Chen X,Zha X,Zhang F

    更新日期:2017-10-30 00:00:00

  • Neurotransmitter role of endogenous morphine in CNS.

    abstract::Endogenous morphine is present in the mammalian brain as determined by gas chromatography/mass spectrometry. The criteria essential for satisfying the definition of morphine as a neurotransmitter are examined. The detection of endogenous morphine-like compounds inside brain neurons by immunocytochemistry and the Ca(+)...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,评审

    doi:

    authors: Guarna M,Ghelardini C,Galeotti N,Stefano GB,Bianchi E

    更新日期:2005-06-01 00:00:00

  • MR proton spectroscopy in liver examinations of healthy individuals in vivo.

    abstract:BACKGROUND:Magnetic resonance proton spectroscopy ((1)H MRS) is a non-invasive method that provides in vivo measurement of metabolic concentrations in body tissue. However, relatively little is known about the potential of 1H MR spectroscopy for the quantification of liver metabolites. The aim of this study was to dete...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Tarasów E,Siergiejczyk L,Panasiuk A,Kubas B,Dzienis W,Prokopowicz D,Walecki J

    更新日期:2002-02-01 00:00:00

  • Advanced Glycation End Products (AGEs) Induce Apoptosis of Fibroblasts by Activation of NLRP3 Inflammasome via Reactive Oxygen Species (ROS) Signaling Pathway.

    abstract::BACKGROUND Type 2 diabetes impairs the healing process and induces apoptosis of fibroblasts, which are thought to be involved in this process. We investigated the possible mechanisms involved in AGEs-induced apoptosis of human dermal fibroblasts. MATERIAL AND METHODS We examined the expression of apoptosis-related pro...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.915806

    authors: Dai J,Chen H,Chai Y

    更新日期:2019-10-06 00:00:00

  • The Association Between Variants of Receptor for Advanced Glycation End Products (RAGE) Gene Polymorphisms and Age-Related Macular Degeneration.

    abstract::BACKGROUND Age-related macular degeneration (AMD) is the leading cause of blindness in people aged 65 years and older in developed countries. The pathogenesis of AMD has been linked to mechanisms involving inflammation, oxidative stress, and basal laminar deposit formation between retinal pigment epithelium (RPE) cell...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.905311

    authors: Banevicius M,Vilkeviciute A,Kriauciuniene L,Liutkeviciene R,Deltuva VP

    更新日期:2018-01-10 00:00:00

  • Reelin Promotes Cisplatin Resistance by Induction of Epithelial-Mesenchymal Transition via p38/GSK3β/Snail Signaling in Non-Small Cell Lung Cancer.

    abstract::BACKGROUND Emerging evidence suggests the involvement of Reelin in chemoresistance in various cancers. However, its function in cisplatin (DDP) sensitivity of non-small cell lung cancer (NSCLC) needs to be investigated. MATERIAL AND METHODS Reelin expression in cisplatin-sensitive A549 cells and cisplatin-resistant NS...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.925298

    authors: Li JM,Yang F,Li J,Yuan WQ,Wang H,Luo YQ

    更新日期:2020-08-07 00:00:00

  • Abnormal Expression of Urea Transporter Protein in a Rat Model of Hepatorenal Syndrome Induced by Succinylated Gelatin.

    abstract:BACKGROUND:Hepatorenal syndrome (HRS) is a serious complication of advanced chronic liver disease. Abdominal compartment syndrome (ACS) occurs with dysfunction of multiple organs when abdominal pressure increases. Here, we report on a novel model of ACS with ascites and a model of HRS in rats to observe the urea transp...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.894232

    authors: Song W,Qi X,Zhang W,Zhao Y,Cao Y,Wang F,Yang C

    更新日期:2015-09-28 00:00:00

  • Fluoxetine differentially suppresses sucrose solution consumption in free-fed and food-deprived rats--reversal by amantadine.

    abstract:BACKGROUND:Clinical use of fluoxetine and similar medications is often associated with appetite suppression and weight loss that may warrant drug discontinuation. It is unclear, however, if fluoxetine-induced consummatory suppression may be influenced by factors such as dietary status and if appetite suppressant effect...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Prendergast MA,Yells DP,Balogh SE,Paige SR,Hendricks SE

    更新日期:2002-10-01 00:00:00

  • Physicians' knowledge and attitudes regarding periconceptional folic acid supplementation: a survey in Southern Israel.

    abstract:BACKGROUND:Pre-conceptional folic acid supplementation is an effective way to reduce the incidence of neural tube defects (NTDs). Primary care providers are an important source of information to promote folic acid intake. This study aimed at evaluating primary care physicians' knowledge and attitudes regarding folic ac...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Abu-Hammad T,Dreiher J,Vardy DA,Cohen AD

    更新日期:2008-05-01 00:00:00

  • Oxidative DNA Damage to Sperm Cells and Peripheral Blood Leukocytes in Infertile Men.

    abstract::BACKGROUND Oxidative DNA damage is associated with male infertility. The aim of this study was to evaluate the oxidative DNA damage of sperm cells and blood leukocytes and to determine the levels of MDA and NO levels in seminal and blood plasma of idiopathic infertile men. MATERIAL AND METHODS The study enrolled 52 pa...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.898631

    authors: Taken K,Alp HH,Eryilmaz R,Donmez MI,Demir M,Gunes M,Aslan R,Sekeroglu MR

    更新日期:2016-11-11 00:00:00

  • Epidermal Growth Factor-Containing Fibulin-Like Extracellular Matrix Protein 1 (EFEMP1) Acts as a Potential Diagnostic Biomarker for Prostate Cancer.

    abstract::BACKGROUND The aim of this study was to detect the expression of epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) and estimate its diagnostic value in prostate cancer (PCa). MATERIAL AND METHODS EFEMP1 expression in serum and urine of patients with PCa, benign controls and health...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.898809

    authors: Shen H,Zhang L,Zhou J,Chen Z,Yang G,Liao Y,Zhu M

    更新日期:2017-01-13 00:00:00

  • Malignant lymphoma of the jaw bone.

    abstract:BACKGROUND:Malignant lymphomas are neoplasms originating from lymphocytes, whose precursor cell or cells are generated during the multipotential differentiation of a stem cell. Extranodal localization most often involves the Waldeyer's ring, nasal cavity, maxillary sinuses, eyeballs, salivary glands, oral cavity, stoma...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Kozakiewicz M,Karolewski M,Kobos JW,Stołecka Z

    更新日期:2003-12-01 00:00:00