Different length (DL) qPCR for quantification of cell killing by UV-induced DNA damage.

Abstract:

:We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.

authors

Rudi K,Hagen I,Johnsrud BC,Skjefstad G,Tryland I

doi

10.3390/ijerph7093376

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

3376-81

issue

9

eissn

1661-7827

issn

1660-4601

pii

ijerph7093376

journal_volume

7

pub_type

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