Abstract:
:A low methyl-esterified pectin (33.2% methyl-esterification degree) was isolated from the tuber of Dioscorea opposita Thunb., which was an edible and medicinal material in China. This pectin (Mw of 1.3 × 104 g/mol) contained the ~59.1% homogalacturonan (HG) and ~38.1% highly branched rhamnogalacturonan I (RG-I) region with possible side chains embracing arabinogalactan II, arabinan or arabinogalactan I. The fragments including HG backbone consisting of → 4)-α-GalpA-(1 → and → 4)-α-GalpA-6-O-methyl-(1 → with molar ratio of ~2:1, and repeating unit of arabinogalactan II side chain composed of α-Araf-(1 → and → 3,6)-β-Galp-(1→, were speculated through methylation analysis and NMR spectra. However, the linkage pattern for RG-I backbone and side chains were indiscernible due to limited resolution of NMR spectra. Besides, the pectin adopted a flexible chain conformation in 0.1 M NaNO3 solution. These results provided a structural basis for study on polysaccharide from D. opposite, which was benefit for development of functional food of yam.
journal_name
Food Chemjournal_title
Food chemistryauthors
Tang W,Liu D,Wang JQ,Huang XJ,Yin JY,Geng F,Nie SPdoi
10.1016/j.foodchem.2021.129899keywords:
["Conformation","Dioscorea opposita Thunb.","Homogalacturonan","Pectin","Rhamnogalacturonan I","Structure"]subject
Has Abstractpub_date
2021-10-15 00:00:00pages
129899eissn
0308-8146issn
1873-7072pii
S0308-8146(21)00905-5journal_volume
359pub_type
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