Abstract：
BACKGROUND:Osteosarcoma (OS) is one of the most common malignant tumors developed in the bone. EZH2 has been found to play pivotal roles in the development of various cancers. LncRNA-ANCR (anti-differentiation ncRNA) has been reported to interact with EZH2 and regulated osteoblast differentiation. Our study aimed to investigate the effect of lncRNA-ANCR on the tumorigenesis of osteosarcoma and explore the underlying molecular mechanism. METHODS:RT-PCR was performed to detect the messenger RNA (mRNA) levels of lncRNA-ANCR, EZH2, p21, and p27 in OS tissues and cell lines. The cell proliferation, transwell invasion, and migration assays were conducted to evaluate the influence of lncRNA-ANCR depletion on the growth of OS cells. RNA pull-down assay was carried out to detect the interaction between lncRNA-ANCR and EZH2. Correlation between the expression of lncRNA-ANCR and the expression of EZH2 were analyzed by cross-tabulation. RESULTS:LncRNA-ANCR is highly expressed in both OS tissues and cell lines. Reduced expression of lncRNA-ANCR inhibited the cell proliferation, invasion, and migration of OS cells. The cell apoptosis rate was also increased with the overexpression of lncRNA-ANCR. Mechanistically, downregulation of lncRNA-ANCR reduced the mRNA level of EZH2 and increased the expression of p21 and p27 at both mRNA and protein levels. LncRNA-ANCR interacted with EZH2 and their expression abundance was positively correlated in OS patients. CONCLUSION:LncRNA-ANCR inhibited the cell proliferation, migration, and invasion of OS cells possibly through interacting with EZH2 and regulating the expression of p21 and p27.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Zhang F,Peng Hdoi
10.1186/s13018-017-0599-7subject
Has Abstractpub_date
2017-07-05 00:00:00pages
103issue
1issn
1749-799Xpii
10.1186/s13018-017-0599-7journal_volume
12pub_type
杂志文章abstract：BACKGROUND:With new minimally-invasive approaches for angular stable plate fixation of proximal humeral fractures, the need for the placement of oblique inferomedial screws ('calcar screw') has increasingly been discussed. The purpose of this study was to investigate the influence of calcar screws on secondary loss of ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-6-50
更新日期：2011-09-24 00:00:00
abstract：BACKGROUND:Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. METHODS:Forty tissue samples from 29 patients undergoing joint replacement for OA were ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-017-0542-y
更新日期：2017-03-28 00:00:00
abstract：BACKGROUND:Since the early 1990s, opioids have been used as a mainstay for pain management surrounding fracture injuries. As opioid dependence has become a major public health issue, it is important to understand what factors can leave patients vulnerable. The purpose of this study was to examine what risk factors, pat...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1233-7
更新日期：2019-09-26 00:00:00
abstract：BACKGROUND:The onset of deep vein thrombosis (DVT) in patients after total hip arthroplasty (THA) may expand or enlarge and subsequently lead to significant mortality. The objective of this study was to investigate potential risk factors for DVT in patients after THA. METHODS:Eligible patients with hip joint diseases ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0716-2
更新日期：2018-01-10 00:00:00
abstract：OBJECTIVES:To investigate clinical effects and manual operational point of Bryan cervical disc prosthesis in Chinese, to observe the stability and range of movement (ROM) post-operatively. METHODS AND MATERIALS:From 2003,12 to 2005,12, Bryan disc prosthesis replacement applied in 83 cases (102 levels) of cervical spon...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-1-13
更新日期：2006-11-04 00:00:00
abstract：:Over the years, management of complex distal radius fractures by closed means has often failed leading to late collapse. We have chosen the principle of ligamentotaxis using external fixation and bone grafting in this study to prevent late complications. Eighty one patients with complex distal radius fractures belongi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-6-23
更新日期：2011-05-21 00:00:00
abstract：BACKGROUND:This study aimed to investigate the incidence and risk factors of postoperative urinary retention (POUR) among elderly patients who underwent hip fracture surgery and to evaluate the effect of indwelling catheterization on the occurrence of POUR. MATERIALS AND METHODS:From January 2012 to January 2015, cons...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1360-1
更新日期：2019-09-18 00:00:00
abstract：BACKGROUND:The use of cephalo-medullary nails (CMN) is a widely accepted management option for the treatment of unstable per-trochanteric hip fractures. A growing body of literature has reported good functional and radiological outcomes in patients managed with a dynamic hip screw supplemented with a trochanteric stabi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,评审
doi：10.1186/s13018-020-02193-5
更新日期：2021-01-11 00:00:00
abstract：BACKGROUND:Exact knowledge of the sacral anatomy is crucial for the percutaneous insertion of iliosacral screws. However, dysplastic anatomical patterns are common. In addition to a preoperative computed tomography (CT) analysis, conventional radiographic measures may help to identify upper sacral dysplasia and to avoi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1472-7
更新日期：2019-12-09 00:00:00
abstract：BACKGROUND:Core decompression (CD) is an important method for the treatment of osteonecrosis of the femoral head (ONFH). Few articles investigate the influence of core decompression on outcomes of ONFH. This study was carried out to observe the safety and effectiveness of core decompression in the treatment of ONFH. M...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,meta分析
doi：10.1186/s13018-019-1359-7
更新日期：2019-09-11 00:00:00
abstract：BACKGROUND:Lumbar floating fusion occasionally causes postoperative adjacent segment disorder (ASD) at lumbosacral level, causing L5 spinal nerve disorder by L5-S1 foraminal stenosis. The disorder is considered to be one of the major outcomes of L5-S1 ASD, which has not been evaluated yet. The present study aimed to ev...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0307-4
更新日期：2015-10-17 00:00:00
abstract：OBJECTIVE:After reverse shoulder arthroplasty (RSA) external and internal rotation will often remain restricted. A postoperative alteration of the biomechanics in the remaining cuff is discussed as a contributing factor to these functional deficits. METHODS:In this study, muscle moment arms as well as origin-to-insert...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-6-42
更新日期：2011-08-16 00:00:00
abstract：BACKGROUND:Ganglion cyst of cruciate ligaments is a rare lesion; the prevalence is 0.3-0.8%. The purpose of this study was to present clinical features of symptomatic posterior cruciate ligament (PCL) cyst, introduce the arthroscopic excision technique, and evaluate the clinical outcome. METHODS:A series of 11 patient...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0798-x
更新日期：2018-04-13 00:00:00
abstract：BACKGROUND:This study was aimed at investigating the causes of lower extremity weaknesses after posterior lumbar spine fusion surgery and looking at subsequent treatment strategies. METHODS:Patients who underwent posterior lumbar spine fusion surgery in the Peking University First Hospital between January 2009 and Dec...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01948-4
更新日期：2020-09-22 00:00:00
abstract：BACKGROUND:The pathogenesis of Monteggia injuries remains controversial. The current study biomechanically explored the pathological changes during Monteggia fractures using finite element analysis. METHODS:Two cadaveric forearm specimens underwent computed tomography in both the prone and supine positions. The images...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0170-3
更新日期：2015-03-04 00:00:00
abstract：:This review analyzes the literature of bone grafts and introduces tissue engineering as a strategy in this field of orthopedic surgery. We evaluated articles concerning bone grafts; analyzed characteristics, advantages, and limitations of the grafts; and provided explanations about bone-tissue engineering technologies...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,评审
doi：10.1186/1749-799X-9-18
更新日期：2014-03-17 00:00:00
abstract：OBJECTIVE:To discuss the sensitivity and specificity of the combinations of multiple factors that work on bone infection after artificial joint, and provide evidence-based medical basis for the early diagnosis of infection after artificial joint. METHODS:A retrospective review was conducted on 35 patients diagnosed wi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01669-8
更新日期：2020-04-25 00:00:00
abstract：BACKGROUND:Entrapment of the superior cluneal nerve (SCN) in an osteofibrous tunnel has been reported as a cause of low back pain (LBP). However, there are few reports on the prevalence of SCN disorder and there are several reports only on favorable outcomes of treatment of SCN disorder on LBP. The purposes of this pro...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-014-0139-7
更新日期：2014-12-31 00:00:00
abstract：:We write in response to Lawton et al.'s (J Orthop Surg Res 12:76, 2017) important systematic review comparing the outcomes of total ankle replacement (TAR) and ankle arthrodesis (AA) after reviewing the existing literature. Traditionally, AA was the gold standard treatment for ankle osteoarthritis but there is renewed...
journal_title：Journal of orthopaedic surgery and research
pub_type： 信件
doi：10.1186/s13018-019-1190-1
更新日期：2019-05-20 00:00:00
abstract：BACKGROUND:Adult Hip Dysplasia (AHD) has been strongly linked with the development of hip osteoarthritis. The complexity and therefore resultant steep learning curve of the Bernese osteotomy for AHD has been well described. The purpose of this study was to analyse the efficacy of a technically less demanding interlocki...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-8-17
更新日期：2013-06-07 00:00:00
abstract：BACKGROUND:Ossification of the posterior longitudinal ligament (OPLL) of the spine is a complex, multifactorial disease. Although several genes that are linked to cervical OPLL susceptibility have been reported, specific genetic studies regarding thoracic OPLL are lacking. Whole-genome sequencing has been considered as...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-1022-8
更新日期：2018-12-22 00:00:00
abstract：BACKGROUND:Posterior transfacet approach has been proved to be a safe and effective access to treat thoracic disc herniation. However, the therapeutic effect and safety of modified transfacet approach for treating thoracic spinal tuberculosis (TST) has not been reported in the clinical literature. In this study, the cl...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0994-8
更新日期：2018-11-20 00:00:00
abstract：BACKGROUND:Joints withstand huge forces, but little is known about subchondral pressures and perfusion during loading. We developed an in vitro calf foot model to explore intraosseous pressure (IOP) and subchondral perfusion during weight bearing. METHODS:Freshly culled calf forefeet were perfused with serum. IOP was ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01754-y
更新日期：2020-06-29 00:00:00
abstract：: Olecranon fractures are a common injury in fractures. The tension band technique for olecranon fractures yields good clinical outcomes; however, it is associated with significant complications. In many patients, implants irritate overlying soft tissues and cause pain. This is mostly due to protrusion of the proximal ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-5-73
更新日期：2010-10-12 00:00:00
abstract：:Acute traumatic patellar dislocation is a common injury in the active and young adult populations. MRI of the knee is recommended in all patients who present with acute patellar dislocation. Numerous operative and non-operative methods have been described to treat the injuries; however, the ideal management of the acu...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,评审
doi：10.1186/1749-799X-7-21
更新日期：2012-06-06 00:00:00
abstract：BACKGROUND:Matrix metalloproteinase (MMPs) synthesized and secreted from connective tissue cells have been thought to participate in degradation of the extracellular matrix. Increased MMPs activities that degrade proteoglycans have been measured in osteoarthritis cartilage. This study aims to suppress the expression of...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-4-45
更新日期：2009-12-24 00:00:00
abstract：BACKGROUND:This study aimed to explore the relationship among different cervical sagittal parameters in asymptomatic volunteers and the correlation between surgical efficacy and difference of presumed and actual postoperative C2-7 Cobbs's angle (C2-7COBB), which was calculated based on preoperative T1 slope (T1S) in pa...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-02016-7
更新日期：2020-11-05 00:00:00
abstract：BACKGROUND:Arthroscopic rotator cuff repairs are mostly secured with suture anchors and often supplemented by footprint decortication. The objectives of this study were to characterize the strength of bone-tendon healing following anchor repair and assess the effect of channeling the supraspinatus (SSP) humeral footpri...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0773-6
更新日期：2018-03-27 00:00:00
abstract：BACKGROUND:The transoral atlantoaxial reduction plate (TARP) is an effective advance in the treatment of atlantoaxial dislocation (AAD) and can enable the performance of anterior atlantoaxial release, reduction, decompression, and internal fixation in a one-stage operation. However, accurate transoral C2 pedicle insert...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,随机对照试验
doi：10.1186/s13018-019-1189-7
更新日期：2019-05-16 00:00:00
abstract：BACKGROUND:A large number of cases of pneumonia caused by novel β-coronavirus emerged in Hubei Province, China, at the end of 2019 and demonstrated great potential for transmission. At present, known independent risk factors include age, diabetes, and other chronic diseases, which may be similar to the patients with ch...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01976-0
更新日期：2020-10-02 00:00:00
