Abstract:
:Ultrasound at 5.0 MHz was noted to be chondro-inductive, with improved SOX-9 gene and COL2A1 protein expression in constructs that allowed for cell-to-cell contact. To achieve tissue-engineered cartilage using macroporous scaffolds, it is hypothesized that a combination of ultrasound at 5.0 MHz and transforming growth factor-β3 induces human mesenchymal stem cell differentiation to chondrocytes. Expression of miR-145 was used as a metric to qualitatively assess the efficacy of human mesenchymal stem cell conversion. Our results suggest that in group 1 (no transforming growth factor-β3, no ultrasound), as anticipated, human mesenchymal stem cells were not efficiently differentiated into chondrocytes, judging by the lack of decrease in the level of miR-145 expression. Human mesenchymal stem cells differentiated into chondrocytes in group 2 (transforming growth factor-β3, no ultrasound) and group 3 (transforming growth factor-β3, ultrasound) with group 3 having a 2-fold lower miR-145 when compared to group 2 at day 7, indicating a higher conversion to chondrocytes. Transforming growth factor-β3-induced chondrogenesis with and without ultrasound stimulation for 14 days in the ultrasound-assisted bioreactor was compared and followed by additional culture in the absence of growth factors. The combination of growth factor and ultrasound stimulation (group 3) resulted in enhanced COL2A1, SOX-9, and ACAN protein expression when compared to growth factor alone (group 2). No COL10A1 protein expression was noted. Enhanced cell proliferation and glycosaminoglycan deposition was noted with the combination of growth factor and ultrasound stimulation. These results suggest that ultrasound at 5.0 MHz could be used to induce chondrogenic differentiation of mesenchymal stem cells for cartilage tissue engineering.
journal_name
J Tissue Engjournal_title
Journal of tissue engineeringauthors
Guha Thakurta S,Budhiraja G,Subramanian Adoi
10.1177/2041731414566529subject
Has Abstractpub_date
2015-01-16 00:00:00pages
2041731414566529issn
2041-7314pii
10.1177_2041731414566529journal_volume
6pub_type
杂志文章abstract::The use of a monoclonal antibody to block the neurite outgrowth inhibitor Nogo-A has been of great interest for promoting axonal recovery as a treatment for spinal cord injury. While several cellular and non-cellular assays have been developed to quantify the bioactive effects of Nogo-A signaling, demand still exists ...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731416629767
更新日期:2016-01-28 00:00:00
abstract::Current materials used for adipose tissue reconstruction have critical shortcomings such as suboptimal volume retention, donor-site morbidity, and poor biocompatibility. The aim of this study was to examine a controlled delivery system of dexamethasone to generate stable adipose tissue when mixed with disaggregated hu...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731417735402
更新日期:2017-10-09 00:00:00
abstract::In vitro-generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose-derived stem cells (hASCs). ASCs were incorporated into methacrylated gelati...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731417744157
更新日期:2017-12-21 00:00:00
abstract::The ability to influence stem cell differentiation is highly desirable as it would help us improve clinical outcomes for patients in various aspects. Many different techniques to achieve this have previously been investigated. This concise study, however, has focused on the topography on which cells grow. Current unce...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731418784098
更新日期:2018-07-11 00:00:00
abstract::The tissue-engineered heart valve may be the ideal valve replacement option but still must overcome challenges in leaflet recellularization. This study sought to investigate the potential for leaflet matrix restoration and repopulation following mononuclear cell seeding and extended periods of bioreactor conditioning....
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731418767216
更新日期:2018-04-10 00:00:00
abstract::Herein, the cytotoxicity of a novel zwitterionic sulfobetaine hydrogel system with a nano-clay crosslinker has been investigated. We demonstrate that careful selection of the composition of the system (monomer to Laponite content) allows the material to be formed into controlled shapes using an extrusion based additiv...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731420967294
更新日期:2020-10-29 00:00:00
abstract::Cartilage defects are a source of pain, immobility, and reduced quality of life for patients who have acquired these defects through injury, wear, or disease. The avascular nature of cartilage tissue adds to the complexity of cartilage tissue repair or regeneration efforts. The known limitations of using autografts, a...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731417697500
更新日期:2017-04-13 00:00:00
abstract::Mineral trioxide aggregate, which comprises three major inorganic components, namely, tricalcium silicate (C3S), dicalcium silicate (C2S), and tricalcium aluminate (C3A), is promising regenerative cement for dentistry. While mineral trioxide aggregate has been successfully applied in retrograde filling, the exact role...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731418807396
更新日期:2018-10-30 00:00:00
abstract::Tendon disease and injuries carry significant morbidity worldwide in both athletic and non-athletic populations. It is estimated that tendon injuries account for 30%-50% of all musculoskeletal injuries globally. Current treatments have been inadequate in providing an accelerated process of repair resulting in high rel...
journal_title:Journal of tissue engineering
pub_type: 杂志文章,评审
doi:10.1177/2041731414549678
更新日期:2014-09-18 00:00:00
abstract::Biomaterial with the dual-functions of bone regeneration and antibacterial is a novel therapy for infective bone defects. Three-dimensional (3D)-printed porous titanium (pTi) benefits bone ingrowth, but its microporous structure conducive to bacteria reproduction. Herein, a multifunctional hydrogel was prepared from d...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731420965797
更新日期:2020-10-23 00:00:00
abstract::Three-dimensional printing technology is getting more attention recently, especially in the craniofacial region. This is a review of literature enlightening the materials that have been used to date and the application of such technology within the scope of maxillofacial surgery. ...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731418770909
更新日期:2018-04-20 00:00:00
abstract::The development of biomaterial surfaces possessing the topographical cues that can promote mesenchymal stem cell recruitment and, in particular, those capable of subsequently directing osteogenic differentiation is of increasing importance for the advancement of tissue engineering. While it is accepted that it is the ...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731414537513
更新日期:2014-05-27 00:00:00
abstract::Stem cells have the capacity to differentiate into various lineages, and the ability to reliably direct stem cell fate determination would have tremendous potential for basic research and clinical therapy. Nanotopography provides a useful tool for guiding differentiation, as the features are more durable than surface ...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.4061/2010/120623
更新日期:2010-08-18 00:00:00
abstract::Donor organ shortage remains a clear problem for many end-stage organ patients around the world. The number of available donor organs pales in comparison with the number of patients in need of these organs. The field of tissue engineering proposes a plausible solution. Using stem cells, a patient's autologous cells, o...
journal_title:Journal of tissue engineering
pub_type: 杂志文章,评审
doi:10.1177/2041731418772949
更新日期:2018-05-10 00:00:00
abstract::Breast carcinomas are complex, three-dimensional tissues composed of cancer epithelial cells and stromal components, including fibroblasts and extracellular matrix. In vitro models that more faithfully recapitulate this dimensionality and stromal microenvironment should more accurately elucidate the processes driving ...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731416660739
更新日期:2016-07-29 00:00:00
abstract::Angiogenesis in adipose tissue is promoted by insulin-like growth factor 1 signaling. We analyzed whether this regulatory mechanism also improves the angiogenic activity of adipose tissue-derived microvascular fragments. Murine adipose tissue-derived microvascular fragments were cultivated for 24 h in the University o...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731419879837
更新日期:2019-09-27 00:00:00
abstract::To reduce excessive scarring in wound healing, electrospun nanofibrous meshes, composed of haloarchaea-produced biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), are fabricated for use as a wound dressing. Three PHBV polymers with different 3HV content are used to prepare either solution-cas...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731420949332
更新日期:2020-08-23 00:00:00
abstract::As part of our clinical tests on bone regeneration using cultured periosteal sheets, here, we prepared cultured periosteal sheets in two types of stem-cell culture media, STK1 and STK3. Human periosteum was expanded either in 1% human serum-supplemented STK1 for 28 days, in 1% human serum-supplemented STK1 for 14 days...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731413509646
更新日期:2013-10-23 00:00:00
abstract::Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to no...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731419881528
更新日期:2019-10-15 00:00:00
abstract::Research into cellular engineered bone grafts offers a promising solution to problems associated with the currently used auto- and allografts. Bioreactor systems can facilitate the development of functional cellular bone grafts by augmenting mass transport through media convection and shear flow-induced mechanical sti...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731419827922
更新日期:2019-02-22 00:00:00
abstract::Tissue engineering skeletal muscle in vitro is of great importance for the production of tissue-like constructs for treating tissue loss due to traumatic injury or surgery. However, it is essential to find new sources of cells for muscle engineering as efficient in vitro expansion and culture of primary myoblasts are ...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731414530138
更新日期:2014-03-27 00:00:00
abstract::The clinical management of large-size cartilage lesions is difficult due to the limited regenerative ability of the cartilage. Different biomaterials have been used to develop tissue engineering substitutes for cartilage repair, including chitosan alone or in combination with growth factors to improve its chondrogenic...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731417697545
更新日期:2017-03-23 00:00:00
abstract::Apoptosis and inflammatory processes may be at the basis of reducing graft survival. Erythropoietin is a tissue-protective hormone with pleiotropic potential, and it interferes with the activities of pro-inflammatory cytokines and stimulates healing following injury, preventing destruction of tissue surrounding the in...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731416671278
更新日期:2016-09-27 00:00:00
abstract::The purpose of this study was to assess the biological revitalization and mechanical integrity of Strattice(™) Reconstructive Tissue Matrix, a porcine-derived acellular dermal matrix, in vivo over time. We expanded the traditional subcutaneous model to incorporate biologic matrix scaffolds large enough to allow evalua...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731413490182
更新日期:2013-05-17 00:00:00
abstract::The tissue-engineered heart valve portends a new era in the field of valve replacement. Decellularized heart valves are of great interest as a scaffold for the tissue-engineered heart valve due to their naturally bioactive composition, clinical relevance as a stand-alone implant, and partial recellularization in vivo....
journal_title:Journal of tissue engineering
pub_type: 杂志文章,评审
doi:10.1177/2041731417726327
更新日期:2017-08-25 00:00:00
abstract::This study evaluated the effect of pulsed low-intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by human dermal fibroblasts encapsulated in alginate. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content, dimethylmethylene blue assay for glycosami...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731415615777
更新日期:2015-11-19 00:00:00
abstract::A range of bioreactors use linear actuators to apply tensile forces in vitro, but differences in their culture environments can limit a direct comparison between studies. The widespread availability of 3D printing now provides an opportunity to develop a 'universal' bioreactor chamber that, with minimal exterior editi...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731420942462
更新日期:2020-09-01 00:00:00
abstract::Spinal fusion has become a common surgical technique to join two or more vertebrae to stabilize a damaged spine; however, the rate of pseudarthrosis (failure of fusion) is still high. To minimize pseudarthrosis, bone morphogenetic protein-2 (BMP2) has been approved for use in humans. In this study, we developed a poly...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731420967591
更新日期:2020-10-24 00:00:00
abstract::It was demonstrated that octacalcium phosphate collagen composite achieved notable bone regeneration in bone defects in preclinical studies. On the basis of the research results, an investigator-initiated exploratory clinical trial was conducted after approval from a local Institutional Review Board. This clinical stu...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731416670770
更新日期:2016-10-03 00:00:00
abstract::The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin. Porcine skin was subject to: sodium chloride (1 M) to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.1% (w/v) so...
journal_title:Journal of tissue engineering
pub_type: 杂志文章
doi:10.1177/2041731417724011
更新日期:2017-08-03 00:00:00