Abstract:
:We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
journal_name
Euro Surveilljournal_title
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletinauthors
Corman VM,Eckerle I,Bleicker T,Zaki A,Landt O,Eschbach-Bludau M,van Boheemen S,Gopal R,Ballhause M,Bestebroer TM,Muth D,Müller MA,Drexler JF,Zambon M,Osterhaus AD,Fouchier RM,Drosten Cdoi
10.2807/ese.17.39.20285-ensubject
Has Abstractpub_date
2012-09-27 00:00:00issue
39eissn
1025-496Xissn
1560-7917journal_volume
17pub_type
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