Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.

Abstract:

:We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

journal_name

Euro Surveill

authors

Corman VM,Eckerle I,Bleicker T,Zaki A,Landt O,Eschbach-Bludau M,van Boheemen S,Gopal R,Ballhause M,Bestebroer TM,Muth D,Müller MA,Drexler JF,Zambon M,Osterhaus AD,Fouchier RM,Drosten C

doi

10.2807/ese.17.39.20285-en

subject

Has Abstract

pub_date

2012-09-27 00:00:00

issue

39

eissn

1025-496X

issn

1560-7917

journal_volume

17

pub_type

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