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Application of reverse transcriptase-nested-PCR for detection of poliovirus in mussels.

Abstract：

:In order to identify polioviruses in molluscs, we hereby propose a method based on precipitation with PEG 6000 followed by the use of a commercial kit (RNAfast II-Molecular System-San Diego) for the extraction and purification of viral RNA. The RT-PCR phase is followed by a second amplification using nested primers to increase the sensitivity and specificity of the method. Tests were carried out on mollusc samples spiked with Poliovirus 1. Results showed that in samples subjected only to one round of PCR it was possible to detect Poliovirus concentrations as small as 10(3)TCID50/ml. The use of nested-PCR makes the system more sensitive and specific enabling the identification of Poliovirus concentrations as small as 1 TCID50/ml.

journal_name

Int J Food Microbiol

journal_title

International journal of food microbiology

authors

De Medici D,Beneduce F,Fiore A,Scalfaro C,Croci L

doi

10.1016/s0168-1605(98)00015-4

subject

Has Abstract

pub_date

1998-03-03 00:00:00

pages

51-6

issue

1-2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(98)00015-4

journal_volume

40

pub_type

杂志文章

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