当前位置： SCI文献检索 > BRITISH JOURNAL OF PHARMACOLOGY期刊下所有文献 > A neuromodulatory role for neuronal nitric oxide in the rabbit renal artery.

A neuromodulatory role for neuronal nitric oxide in the rabbit renal artery.

Abstract：

:1. The effects of the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on vasoconstrictor responses to transmural electrical nerve stimulation and noradrenaline were examined in the rabbit isolated renal artery with and without an intact endothelium. In addition, the effect of removing the endothelium from the renal artery on vasoconstrictor responses to transmural electrical nerve stimulation and noradrenaline was also investigated. Immunohistofluorescence techniques were carried out to determine if there were any nitrergic nerves supplying the renal artery. 2. The vasoconstriction produced in response to transmural electrical nerve stimulation (2-64 Hz) was significantly enhanced in the presence of L-NAME (3 x 10(-6), 10(-5), 3 x 10(-5) and 10(-4) M). 3. L-NAME (3 x 10(-6), 10(-5), 3 x 10(-5) and 10(-4) M) did not significantly affect the maximum vasoconstriction produced in response to noradrenaline. However, the noradrenaline dose-response curve was significantly shifted to the left by the addition of L-NAME (3 x 10(-6), 10(-5), 3 x 10(-5) and 10(-4) M). 4. The increase in the amplitude of the vasoconstriction, produced in response to transmural electrical nerve stimulation (16 Hz) and noradrenaline (10(-5) M) in the presence of L-NAME (10(-5) M) was not observed when L-arginine (10(-3) M) was added in addition to L-NAME (10(-5) M). 5. Removing the endothelium did not significantly affect the response to transmural electrical nerve stimulation (1-64 Hz). The maximum vasoconstriction in response to noradrenaline was also unaffected by the removal of the endothelium. The pD2 value for noradrenaline obtained from vessels with no endothelium was significantly greater than the pD2 value obtained from vessels with an intact endothelium (5.90 +/- 0.11 and 5.16 +/- 0.03, respectively). 6. On renal artery segments with no endothelium L-NAME (3 x 10(-5) M) significantly enhanced the response to transmural electrical nerve stimulation (2-64 Hz). L-NAME did not affect the maximum response to noradrenaline. However, there was a significant shift to the right of the noradrenaline doseresponse curve in the presence of L-NAME (3 x 10(-5) M). 7. Both nitric oxide synthase-containing and NADPH-diaphorase stained nerves were located on the adventitial-medial border of the rabbit renal artery. 8. The present study has suggested a presynaptic inhibitory action for nitric oxide (probably derived from identified perivascular nitrergic nerves), on perivascular sympathetic vasoconstrictor nerve mediated responses of the rabbit renal artery. In contrast, the enhancement of the response to noradrenaline by L-NAME can be attributed to inhibition of the synthesis of endothelium-derived nitric oxide.

journal_name

Br J Pharmacol

journal_title

British journal of pharmacology

authors

Vials AJ,Crowe R,Burnstock G

doi

10.1038/sj.bjp.0701141

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

213-20

issue

eissn

0007-1188

issn

1476-5381

journal_volume

121

pub_type

杂志文章

在线工具

A neuromodulatory role for neuronal nitric oxide in the rabbit renal artery.