Abstract:
PURPOSE:Supramolecular Biovectors (SMBV) consist of cross-linked cationic nanoparticles surrounded by a lipid membrane. The purpose was to study the structure of the lipid membrane and to characterise its interaction with the nanoparticles in order to differentiate SMBV from other polymer/lipid associations. METHODS:The interaction of lipids with the nanoparticle surface was studied using zeta potential. Fluorescence Energy Transfer (FET) and Fluorescence Microscopy. SMBV were compared to liposomes and mixtures nanoparticles/liposomes. Finally the structure of SMBV was visualised by Electron Microscopy. RESULTS:Zeta potential measurements showed that lipids on SMBV had a pronounced shielding effect on the surface charge. This was not the case for mixtures of nanoparticles and liposomes. FET experiments confirmed these results indicating that, for SMBV, the lipids are much closer to the nanoparticle surface. SMBV Fluorescence microscopy on model microparticles showed a lipid crown on SMBV that was confirmed by electron microscopy on SMBV nanoparticles. CONCLUSIONS:Results show that in case of SMBV lipids are strongly adsorbed on the polysaccharide core surface probably due to ionic/hydrophobic interactions. The resulting supramolecular structure is a spherical cationic polysaccharide particle surrounded by a phospholipid/cholesterol layer.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
De Miguel I,Imbertie L,Rieumajou V,Major M,Kravtzoff R,Betbeder Ddoi
10.1023/a:1007504124603subject
Has Abstractpub_date
2000-07-01 00:00:00pages
817-24issue
7eissn
0724-8741issn
1573-904Xjournal_volume
17pub_type
杂志文章abstract:PURPOSE:Scutellarin, a flavonoid derived from the plant Erigeron breviscapus, is currently widely used to treat cerebrovascular diseases, liver-related diseases, and hyperlipidemia in china and other East Asian countries. This study was to investigate the effect of scutellarin on the uptake of rosuvastatin in HEK293T c...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-020-02950-5
更新日期:2020-10-29 00:00:00
abstract::The influence of the jejunal bloodflow on the absorption of theophylline was investigated. The bloodflow through a segment under investigation was varied by changing the systemic blood pressure by means of a donor blood infusion into the jugular vein or by an infusion of isoprenaline or levarterenol into a femoral vei...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/A:1016374508542
更新日期:1984-01-01 00:00:00
abstract:PURPOSE:To show that atomic force microscopy (AFM) can be used to directly study the electrostatic charging and dissipation of single pharmaceutical particles. MATERIALS AND METHODS:Particles of lactose attached to AFM cantilevers were charged on a glass surface at a relative humidity (RH) of 0.1%. By recording force-...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-006-9230-z
更新日期:2007-06-01 00:00:00
abstract::The relationship between pKa and skin irritation in man is studied for a homologous series of benzoic acid derivatives, which permeate through human skin at comparable rates (15-88 micrograms/cm2/hr). Skin irritation and pKa are correlated for pKa less than or equal to 4. Laser Doppler velocimetric assessment of skin ...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015931105660
更新日期:1988-10-01 00:00:00
abstract::The hair follicle, hair shaft, and sebaceous gland collectively form what is recognized as the pilosebaceous unit. This complex, three-dimensional structure within the skin possesses a unique biochemistry, metabolism and immunology. Recent studies have focused on the hair follicle as a potential pathway for both local...
journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
doi:10.1023/a:1016250422596
更新日期:1995-02-01 00:00:00
abstract:PURPOSE:The purpose of this work was to study the influence of cell differentiation on the mRNA expression of transporters and channels in Caco-2 cells and to assess Caco-2 cells as a model for carrier-mediated drug transport in the intestines. METHOD:Gene mRNA expression was measured using a custom-designed microarra...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1022282221530
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:Induction of cell-mediated immune (CMI) responses is crucial for vaccine-mediated protection against difficult vaccine targets, e.g., Chlamydia trachomatis (Ct). Adjuvants are included in subunit vaccines to potentiate immune responses, but many marketed adjuvants stimulate predominantly humoral immune response...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-017-2180-9
更新日期:2017-08-01 00:00:00
abstract:PURPOSE:To measure aggregate and particle formation in tumor necrosis factor-alpha (TNF-α) inhibitors etanercept, adalimumab and certolizumab pegol product samples after exposure to freezing temperature conditions similar to storage conditions previously observed in patients' homes. METHODS:TNF-α inhibitors in their o...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-017-2341-x
更新日期:2018-02-05 00:00:00
abstract::Micelle-associated epoetin is still a possible explanation for the upsurge of pure red blood cell aplasia associated with subcutaneous use of epoetin alpha in patients with chronic renal failure. ...
journal_title:Pharmaceutical research
pub_type: 评论,信件
doi:10.1007/s11095-006-9573-5
更新日期:2006-03-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1011935222652
更新日期:1998-02-01 00:00:00
abstract:PURPOSE:Oral bioavailability for antisense oligonucleotides has recently been reported but the mechanistic details are not known. The proposed oral delivery of nucleic acids will, therefore, require an understanding of the membrane binding interactions, cell uptake and transport of oligonucleotides across the human gas...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016002606705
更新日期:1996-07-01 00:00:00
abstract:PURPOSE:To assess the effects of co-delivering osteoinductive DNA and/or small interfering RNA in directing the osteogenic differentiation of human adipose-derived stem cells (hADSCs) using a combinatorial, non-viral gene delivery approach. METHODS:hADSCs were transfected using combinations of the following genes: BMP...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-011-0406-9
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:Evidence suggests that uremic toxins such as hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS), and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) promote the progression of renal failure by damaging tubular cells via rat organic anion transporter 1 (rOat1) and rOat3 on the basolateral membrane of ...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-005-2486-x
更新日期:2005-04-01 00:00:00
abstract:PURPOSE:Study the radiolysis of solid-state metoclopramide hydrochloride at various absorbed doses. Elucidate the structure of the degradation products to gain information on the radiolysis mechanisms. METHODS:Solid-state metoclopramide samples were irradiated at several doses with gamma rays and high-energy electrons...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-006-0019-x
更新日期:2006-06-01 00:00:00
abstract::The pharmacokinetics of Sb was examined in 29 patients with cutaneous leishmaniasis following the intramuscular administration of a dose of sodium stibogluconate equivalent to 600 mg of Sb. Blood was sampled at different time intervals from each patient and Sb was measured in whole blood by electrothermal atomic absor...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016251023427
更新日期:1995-01-01 00:00:00
abstract:PURPOSE:A novel photo-activated targeted chemotherapy was developed by photochemical internalization (PCI) of glutathione-sensitive polymeric micelles incorporating camptothecin (CPT) prepared from thiolated CPT (CPT-DP) and thiolated poly(ethylene glycol)-b-poly(glutamic acid) (PEG-b-P(Glu-DP)) METHODS: PEG-b-P(Glu-DP...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-008-9712-2
更新日期:2009-01-01 00:00:00
abstract::The Office of Generic Drugs has ensured the high quality of generic products based upon two requirements: pharmaceutical equivalence and bioequivalence to the reference listed drug (RLD). This paradigm has been used with success toward ensuring quality generic drug products that provide the same therapeutic benefit as...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-011-0397-6
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:Basal insulins with improved kinetic properties can potentially be produced using acylation by fatty acids that enable soluble, high-molecular weight complexes to form post-injection. A series of insulins, acylated at B29 with fatty acids via glutamic acid spacers, were examined to deduce the structural require...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-012-0739-z
更新日期:2012-08-01 00:00:00
abstract:PURPOSE:This paper attempts to correlate the hydrophobic contribution constants (faa) of 21 amino acids in small peptides with commonly used physiochemical parameters. These faa constants can then be used to predict hydrophobicity change in peptides when any one of the amino acid residue is substituted with another. M...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016008102587
更新日期:1996-08-01 00:00:00
abstract:PURPOSE:To prepare acylated exenatide analogues and investigate their biological properties for guiding the development of PLGA formulations of exenatide. METHODS:The acylated exenatide analogues were prepared by reaction with glycolic acid (GA), one constitutional unit of PLGA, and characterized by HPLC-MS/MS and Cir...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-014-1298-2
更新日期:2014-08-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1007512617139
更新日期:2000-04-01 00:00:00
abstract::The synthesis of an homologous series of new water-soluble derivatives of pilocarpine is described. The new compounds, referred to as soft quaternary salts, are water soluble by virtue of a cationic ammonium head and their lipophilicity can be modulated by manipulating the size and the nature of the substituent in the...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015847103862
更新日期:1992-03-01 00:00:00
abstract:PURPOSE:The use of dendrimers for biomedical applications has emerged with promising results. 2G-NN16 is a carbosilane dendrimer with sixteen positive charges per molecule tested to be capable to bind and release antisense oligonucleotides (ODNs) and small interference RNA (siRNA) in vitro. In spite of low cytotoxicity...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-008-9776-z
更新日期:2009-03-01 00:00:00
abstract:PURPOSE:Under different charging conditions, particles can be either attracted or repulsed by each other, causing powder agglomeration or segregation. Such behavior can be detrimental in many processes aimed at achieving particulate mixture homogeneity. Consequently, the effects of electrostatic charges on mixing kinet...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-006-9934-0
更新日期:2006-05-01 00:00:00
abstract:PURPOSE:Exenatide, a potent insulinotropic agent, can be used for the treatment of non-insulin-dependent diabetes mellitus. However, the need for frequent injections seriously limits its therapeutic utility. The aim of present report was to develop an orally available exenatide formulation using goblet cell-targeting n...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-014-1513-1
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:Platinum-based therapies are the first line treatments for most types of cancer including ovarian cancer. However, their use is associated with dose-limiting toxicities and resistance. We report initial translational studies of a theranostic nanoemulsion loaded with a cisplatin derivative, myrisplatin and pro-a...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-015-1660-z
更新日期:2015-08-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016033124819
更新日期:1996-12-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016121319668
更新日期:2002-06-01 00:00:00
abstract::The effects of process and storage conditions of solid-state emulsions were studied. Oil-in-water emulsions may be prepared from solid state emulsions by adding an aqueous phase to the solid. Solid-state emulsions are prepared by processing an oil phase and an aqueous solution of matrix material via a solvent removal ...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1018983227234
更新日期:1993-07-01 00:00:00
abstract::The influence of varying protein and fat content in milk of New Zealand White rabbits on the milk-to-plasma drug concentration (M/P) ratio of diazepam was studied. At various time points after littering, a bolus dose (1.5 mg/kg) followed by a 26-hr infusion (1.8 mg/h) of diazepam was administered to freely moving rabb...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015809418284
更新日期:1992-10-01 00:00:00