Abstract:
:An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.). The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution. Both enzymes are localized exclusively in the cell wall. Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls. The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth.
journal_name
Plantajournal_title
Plantaauthors
Slocum RD,Furey MJ 3rddoi
10.1007/BF00197744subject
Has Abstractpub_date
1991-02-01 00:00:00pages
443-50issue
3eissn
0032-0935issn
1432-2048journal_volume
183pub_type
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