Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following subretinal injection in the rat.

Abstract:

:We recently reported that different purification methods of recombinant adeno-associated virus type 2 (rAAV2) affect the transduction characteristics following subretinal injection. In this study, we examined the roles of contaminant proteins from the HEK-293 cells and helper adenovirus, inactivation of helper adenovirus and cell stress induced by DNA-damaging agents in rAAV-mediated retinal transduction. Our results showed that contaminating factors/proteins resulting from the helper E1 deleted adenovirus are possibly responsible for efficient RPE transduction. Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases.

journal_name

Vision Res

journal_title

Vision research

authors

Shen WY,Lai YK,Lai CM,Rakoczy PE

doi

10.1016/j.visres.2003.09.032

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

339-48

issue

4

eissn

0042-6989

issn

1878-5646

pii

S0042698903006254

journal_volume

44

pub_type

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