Phagocytosis of nanoparticles by human immunodeficiency virus (HIV)-infected macrophages: a possibility for antiviral drug targeting.

abstract：PURPOSE:The purpose of this study was to determine the existence of insulin-degrading enzyme (EC 3.4.22.11) (IDE) in rat intestinal enterocytes. METHODS:Subcellular fractionation, biochemical characterization, immunoprecipitation, and western blots were employed. RESULTS:Insulin-degrading activity was localized in th...

journal_title：Pharmaceutical research

authors： Bai JP,Chang LL

更新日期：1995-08-01 00:00:00

abstract：PURPOSE:This study aimed to investigate the impact of the size of X-ray iodinated contrast agent in nano-emulsions, on their toxicity and fate in vivo. METHODS:A new compound, triiodobenzoate cholecalciferol, was synthetized, formulated as nano-emulsions, and followed after i.v. administration in mice by X-ray imaging...

journal_title：Pharmaceutical research

authors： Attia MF,Anton N,Akasov R,Chiper M,Markvicheva E,Vandamme TF

更新日期：2016-03-01 00:00:00

abstract：PURPOSE:N-terminal site-specific mono-PEGylation of recombinant human epidermal growth factor (EGF) was accomplished using polyethyleneglycol (PEG) derivatives (Mw = 2000 and 5000) through a reactive terminal aldehyde group. METHODS:The site-specific PEG conjugation was conducted ata slightly acidic pH condition (pH 5...

journal_title：Pharmaceutical research

authors： Lee H,Jang IH,Ryu SH,Park TG

更新日期：2003-05-01 00:00:00

abstract：PURPOSE:The aim of the study is to investigate the effect of polymer blending on entrapment and release of ganciclovir (GCV) from poly(D,L-lactide-co-glycolide) (PLGA) microspheres using a set of empirical equations. METHODS:Two grades of PLGA, PLGA 7525 [D,L-lactide:glycolide(75:25), MW 90,000-126,000 Da] and Resomer...

journal_title：Pharmaceutical research

authors： Duvvuri S,Gaurav Janoria K,Mitra AK

更新日期：2006-01-01 00:00:00

abstract：PURPOSE:To develop a semi-mechanistic population pharmacokinetic model based on gastric emptying function for acetaminophen plasma concentration in critically ill patients tolerant and intolerant to enteral nutrition before and after prokinetic therapy. METHODS:Acetaminophen plasma concentrations were available from a...

journal_title：Pharmaceutical research

authors： Ogungbenro K,Vasist L,Maclaren R,Dukes G,Young M,Aarons L

更新日期：2011-02-01 00:00:00

abstract：PURPOSE:This study was carried out to examine the blood-brain barrier (BBB) transport of human basic fibroblast growth factor (bFGF) and investigate its mechanism. METHODS:The BBB transport of 125I-bFGF was measured by several in vivo methods including intravenous administration, in situ internal carotid artery perfus...

journal_title：Pharmaceutical research

authors： Deguchi Y,Naito T,Yuge T,Furukawa A,Yamada S,Pardridge WM,Kimura R

更新日期：2000-01-01 00:00:00

abstract：PURPOSE:Cannabinoids have been associated with tumor regression and apoptosis of cancer cells. Recently, we have shown that R(+)-methanandamide (R(+)-MA) induces apoptosis of H4 human neuroglioma cells via a mechanism involving de novo expression of the cyclooxygenase-2 (COX-2) enzyme. The present study investigated a ...

journal_title：Pharmaceutical research

authors： Eichele K,Weinzierl U,Ramer R,Brune K,Hinz B

更新日期：2006-01-01 00:00:00

abstract：PURPOSE:Deposition characteristics of MDI and DPI aerosols were compared throughout the conducting airways for the first time using a combination of in vitro experiments and a newly developed stochastic individual path (SIP) model for different inhalation profiles. METHODS:In vitro experiments were used to determine i...

journal_title：Pharmaceutical research

authors： Longest PW,Tian G,Walenga RL,Hindle M

更新日期：2012-06-01 00:00:00

abstract：PURPOSE:To investigate, for the first time, the performance of a dry powder inhaler (DPI, Aerolizer(®)) in the case of a model drug (i.e. albuterol sulphate) formulated with spray dried mannitol carrier particles with homogeneous shape and solid-state form but different sizes. METHODS:Spray dried mannitol (SDM) partic...

journal_title：Pharmaceutical research

authors： Kaialy W,Hussain T,Alhalaweh A,Nokhodchi A

更新日期：2014-01-01 00:00:00

abstract：:Unsymmetrical aryl(heteroaryl)methyl isopropyl ester analogues of nifedipine, in which the 2-nitrophenyl group at C-4 is replaced by a 2- or 3-pyridyl substituent, were synthesized and evaluated as calcium-channel antagonists using guinea pig ileal longitudinal smooth muscle. The point of attachment of the C-4 pyridyl...

journal_title：Pharmaceutical research

authors： Akula MR,Matowe WC,Wolowyk MW,Knaus EE

更新日期：1990-09-01 00:00:00

abstract：PURPOSE:Calculation of the ideal solubility of a crystalline solute in a liquid solvent requires knowledge of the difference in the molar heat capacity at constant pressure of the solid and the supercooled liquid forms of the solute, delta Cp. Since this parameter is not usually known, two assumptions have been used to...

journal_title：Pharmaceutical research

authors： Neau SH,Bhandarkar SV,Hellmuth EW

更新日期：1997-05-01 00:00:00

abstract：PURPOSE:The objective of this study was to determine the pharmacokinetics and safety for NX1838 following injection into the vitreous humor of rhesus monkeys. METHODS:Plasma and vitreous humor pharmacokinetics were determined following a single bilateral 0.25, 0.50, 1.0, 1.5, or 2.0 mg/eye dose. In addition, the pharm...

journal_title：Pharmaceutical research

authors： Drolet DW,Nelson J,Tucker CE,Zack PM,Nixon K,Bolin R,Judkins MB,Farmer JA,Wolf JL,Gill SC,Bendele RA

更新日期：2000-12-01 00:00:00

abstract：PURPOSE:The development of a new two-dimensional (2D) model to predict follicular permeation, with integration into a recently reported multi-scale model of transdermal permeation is presented. METHODS:The follicular pathway is modelled by diffusion in sebum. The mass transfer and partition properties of solutes in li...

journal_title：Pharmaceutical research

authors： Kattou P,Lian G,Glavin S,Sorrell I,Chen T

更新日期：2017-10-01 00:00:00

abstract：PURPOSE:This work investigated the effect of relative humidity (RH) on bipolar electrostatic charge profiles of dry powder inhaler aerosols using the Bipolar Charge Analyzer (BOLAR). METHODS:Two commercial products, Pulmicort® (400 μg, budesonide) and Bricanyl® (500 μg, terbutaline sulfate) Turbuhaler®, were used as m...

journal_title：Pharmaceutical research

authors： Yu J,Wong J,Ukkonen A,Kannosto J,Chan HK

更新日期：2017-08-01 00:00:00

abstract：:A sensitive activity assay for high volume evaluation of human renin inhibitory peptides (RIPs) in rat sera (range 2-80 ng/ml) was developed based on the low affinity of RIPs to rat renin and their high affinity to human renin. The utility of this activity assay was tested by measuring concentrations of a human RIP, U...

journal_title：Pharmaceutical research

authors： Ruwart MJ,Sharma SK,Harris DW,Lakings DB,Rush BD,Wilkinson KF,Cornette JC,Evans DB,Friis JM,Cook KJ

更新日期：1990-04-01 00:00:00

abstract：PURPOSE:To measure the diffusion of nanometer polymersomes through tissue engineered human oral mucosa. METHODS:In vitro models of full thickness tissue engineered oral mucosa (TEOM) were used to assess the penetration properties of two chemically different polymersomes comprising two of block copolymers, PMPC-PDPA an...

journal_title：Pharmaceutical research

authors： Hearnden V,Lomas H,Macneil S,Thornhill M,Murdoch C,Lewis A,Madsen J,Blanazs A,Armes S,Battaglia G

更新日期：2009-07-01 00:00:00

abstract：PURPOSE:The objective of this study was to develop and evaluate a pulsatile drug delivery system based on an impermeable capsule body filled with drug and an erodible plug placed in the opening of the capsule body. METHODS:The erodible plugs were either prepared by direct compression followed by placing the tablets in...

journal_title：Pharmaceutical research

authors： Krögel I,Bodmeier R

更新日期：1998-03-01 00:00:00

abstract：PURPOSE:Using human lung cancer cells, we evaluated the involvement of plasminogen activator inhibitor-1 (PAI-1) in the anti-invasive action of cannabidiol, a non-psychoactive cannabinoid. METHODS:Invasion was quantified by a modified Boyden chamber assay. PAI-1 protein in cell culture media and PAI-1 mRNA were determ...

journal_title：Pharmaceutical research

authors： Ramer R,Rohde A,Merkord J,Rohde H,Hinz B

更新日期：2010-10-01 00:00:00

abstract：PURPOSE:To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells. METHODS:Copolymers of phenylboronic acid-functional poly(amid...

journal_title：Pharmaceutical research

authors： Hujaya SD,Engbersen JF,Paulusse JM

更新日期：2015-09-01 00:00:00

abstract：PURPOSE:This prospective study aimed to evaluate the effects of genetic polymorphisms in sulindac-related metabolizing enzyme genes including FMO3 and AOX1 on the population pharmacokinetics of sulindac in 58 pregnant women with preterm labor. METHODS:Plasma samples were collected at 1.5, 4, and 10 h after first oral ...

journal_title：Pharmaceutical research

authors： Sung JW,Yun HY,Park S,Kim YJ,Yee J,Lee KE,Song B,Chung JE,Gwak HS

更新日期：2020-01-28 00:00:00

abstract：PURPOSE:The aim of this study was to investigate the effect of cyclodextrins (beta-CD, HP-beta-CD and (SBE)7m-beta-CD), and co-administration of a water-soluble polymer (HPMC) and cyclodextrins, on the oral bioavailability of glibenclamide in dogs. METHODS:Effects of cyclodextrins on the aqueous solubility of glibencl...

journal_title：Pharmaceutical research

authors： Savolainen J,Järvinen K,Taipale H,Jarho P,Loftsson T,Järvinen T

更新日期：1998-11-01 00:00:00

abstract：PURPOSE:The aim was to investigate the influence of polymer content and molecular weight on the morphology and heat- and moisture-induced transformations, as indicators of stability, of spray-dried composite particles of amorphous lactose and poly(vinylpyrrolidone) (PVP). METHODS:Amorphous lactose and composite partic...

journal_title：Pharmaceutical research

authors： Berggren J,Alderborn G

更新日期：2003-07-01 00:00:00

abstract：PURPOSE:Preparation of Nanographene oxide (NGO) - Gelatin hybrids for efficient treatment of Neuroblastoma. METHODS:Nanohybrids were prepared via non-covalent interactions. Spectroscopic tools have been used to discriminate the chemical states of NGO prior and after gelatin coating, with UV visible spectroscopy reveal...

journal_title：Pharmaceutical research

authors： Makharza S,Vittorio O,Cirillo G,Oswald S,Hinde E,Kavallaris M,Büchner B,Mertig M,Hampel S

更新日期：2015-06-01 00:00:00

abstract：:Magnetic nanoparticles are useful as contrast agents for magnetic resonance imaging (MRI). Paramagnetic contrast agents have been used for a long time, but more recently superparamagnetic iron oxide nanoparticles (SPIOs) have been discovered to influence MRI contrast as well. In contrast to paramagnetic contrast agent...

journal_title：Pharmaceutical research

authors： Rümenapp C,Gleich B,Haase A

更新日期：2012-05-01 00:00:00

abstract：PURPOSE:Previous studies by other investigators have shown an enhancement of mitomycin C (MMC) activity at acidic extracellular pH (pHe) in monolayer cultures of human cells. The goal of the present study was to determine if the efficacy of intravesical MMC therapy in patients treated for superficial bladder cancer can...

journal_title：Pharmaceutical research

authors： Yen WC,Schmittgen T,Au JL

更新日期：1996-12-01 00:00:00

abstract：PURPOSE:This study aimed to characterize the transepithelial transport of miltefosine (HePC), the first orally effective drug against visceral leishmaniasis, across the intestinal barrier to further understand its oral absorption mechanism. MATERIALS AND METHODS:Caco-2 cell monolayers were used as an in vitro model of...

journal_title：Pharmaceutical research

authors： Ménez C,Buyse M,Dugave C,Farinotti R,Barratt G

更新日期：2007-03-01 00:00:00

abstract：PURPOSE:To investigate cellular uptake pathways of novel anionic siRNA-lipoplexes as a function of formulation composition. METHODS:Anionic formulations with anionic lipid/Ca(2+)/siRNA ratio of 1.3/2.5/1 (AF1) and 1.3/0.3/1 (AF2) were utilized. Uptake mechanisms were investigated using uptake inhibition and co-localiz...

journal_title：Pharmaceutical research

authors： Kapoor M,Burgess DJ

更新日期：2013-04-01 00:00:00

abstract：PURPOSE:The combination of morphine and gabapentin seems promising for the treatment of postoperative and neuropathic pain. Despite the well characterised pharmacodynamic interaction, little is known about possible pharmacokinetic interactions. The aim of this study was to evaluate whether co-administration of the two ...

journal_title：Pharmaceutical research

authors： Papathanasiou T,Juul RV,Gabel-Jensen C,Kreilgaard M,Lund TM

更新日期：2016-11-01 00:00:00

abstract：PURPOSE:A series of studies was conducted to evaluate the preclinical pharmacokinetics of SB-239063 (trans-1-(4-hydroxycyclohexyl)-4-(4-fluorophenyl)-5-[(2-methoxy)pyrimidin-4-yl] imidazole), a potent and selective p38 MAP kinase inhibitor. METHODS:SB-239063 was administered both i.v. and p.o. in the rat, dog, cynomol...

journal_title：Pharmaceutical research

authors： Ward KW,Proksch JW,Azzarano LM,Salyers KL,McSurdy-Freed JE,Molnar TM,Levy MA,Smith BR

更新日期：2001-09-01 00:00:00

abstract：PURPOSE:For the assessment of bioequivalence it is assumed that drug clearance in each subject on each of the study days is the same and any observed differences in AUC and/or Cmax between a brand and generic formulation are due to differences in bioavailability. We hypothesized that this assumption was invalid for hig...

journal_title：Pharmaceutical research

authors： Tsang YC,Pop R,Gordon P,Hems J,Spino M

更新日期：1996-06-01 00:00:00