Abstract:
:1. A method is presented which enables the dissociation constant (KI) of a competitive, pseudo-irreversible or non-competitive antagonist-receptor complex to be estimated without knowledge of agonist concentrations. 2. The technique has been tested using sets of concentration-response data which simulated these various types of antagonism. 3. The points for each set of simulated data could be plotted both as agonist concentration-response curves at fixed antagonist concentrations and vice versa, producing paired data sets. 4. pKI-values were estimated from such paired data sets using appropriate graphical and computer curve-fitting methods. 5. For competitive antagonism, for each paired data set the computer curve-fitting techniques gave the same value for pKI, assuming drug-receptor interaction to be 1:1 and agonist concentrations to be known. 6. When agonist concentrations were assumed unknown, pKIS could not be estimated by the conventional method (using agonist dose-ratios) but could still be obtained (for competitive, pseudo-irreversible and non-competitive antagonism) by the new method. 7. This new method should be especially useful for measuring dissociation constants of antagonists against neuronally- or ionophoretically-released agonists. It may also be useful when agonist is applied exogenously, especially if suitable drugs are not available to block agonist uptake and/or metabolism.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Mackay Ddoi
10.1111/j.1476-5381.1994.tb14047.xsubject
Has Abstractpub_date
1994-01-01 00:00:00pages
219-26issue
1eissn
0007-1188issn
1476-5381journal_volume
111pub_type
杂志文章abstract:BACKGROUND AND PURPOSE:7-Ketocholesterol, an oxysterol present in atherosclerotic lesions, induces smooth muscle cell (SMC) death, thereby destabilizing plaques. Statins protect patients from myocardial infarction, though they induce SMC apoptosis. We investigated whether statins and 7-ketocholesterol exerted additive ...
journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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更新日期:1981-11-01 00:00:00
abstract::1. The administration of an analgesic dose (10 mg/kg, s.c.) of morphine increased the concentrations of the dopamine metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the brains of normal mice, and the time course of the change in the DOPAC concentration corresponded approximately to t...
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abstract:BACKGROUND AND PURPOSE:There is growing evidence that inflammation plays a major role in the pathogenesis of neural damage caused by deposition of amyloid β (Aβ) in the brain. Nimodipine has received attention as a drug that might improve learning and reduce cognitive deficits in Alzheimer's disease, but the mechanism ...
journal_title:British journal of pharmacology
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更新日期:1989-07-01 00:00:00
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journal_title:British journal of pharmacology
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abstract::1 The effects of gamma-aminobutyric acid (GABA) and related substances were examined in guinea-pig ileum longitudinal muscle.2 GABA at doses ranging from 10(-7) M to 3 x 10(-6) M elicited a relaxation while at higher doses (3 x 10(-6) M - 10(-4) M), as previously described, it caused a contraction followed by relaxati...
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更新日期:2007-07-01 00:00:00
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更新日期:2004-12-01 00:00:00
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更新日期:2000-09-01 00:00:00