Abstract：
:Background Insulin-like growth factor 1 (IGF-1) is an autocrine regulator of carcinoid tumors. Blockade of IGF-1 signaling has been proposed as a therapeutic target in the treatment of patients with carcinoid syndrome. We hypothesized that the induction of parallel raf-1/MEK1 pathways will block IGF-1-mediated chromogranin A (CgA) maintenance. Methods Human gastrointestinal carcinoid tumor cells (BON) were treated with IGF-1 (0-500 ng/mL). Raf-1/MEK1 activation was achieved with an estrogen-inducible raf-1 vector that was transduced into BON cells. Activation of IGF-1/raf-1 pathways was determined by phosphorylation of downstream targets p70s6 and ERK1/2. The secreted and intercellular levels of CgA were measured in conditioned media and whole cell extracts by Western and enzyme-linked immunosorbent assay analysis. Results IGF-1 and raf-1 pathways were activated successfully in BON cells, as shown by high levels of phosphorylated p70s6 and phosphorylated ERK1/2, respectively. Treatment of BON cells with IGF-1 stimulated the release of CgA, while high intracellular CgA levels were maintained. The activation of raf-1/MEK1 reversed the effect of IGF-1 treatment by the depletion of intracellular CgA. Conclusions The induction of the raf-1/MEK1 pathway blocks IGF-1-mediated intracellular neuroendocrine hormone regulation. Therefore, raf-1/MEK1 activation may be a viable method to block IGF-1-mediated cellular effects and serve as a therapeutic target in gastrointestinal carcinoid tumors.
journal_name
Surgeryjournal_title
Surgeryauthors
Van Gompel JJ,Chen Hdoi
10.1016/j.surg.2004.06.061subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1297-302issue
6eissn
0039-6060issn
1532-7361pii
S0039606004005537journal_volume
136pub_type
杂志文章相关文献
SURGERY文献大全abstract：:Much can be learned about the history of vascular surgery by tracing the treatment of aneurysms. Rudolph Matas played a major role in the development of the current therapy for these lesions. In the centennial of the signal case that set him on the road to surgical immortality, the details and setting of this operatio...
journal_title：Surgery
pub_type： 传,历史文章,杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：BACKGROUND:Despite nationwide campaigns to increase the use of helmets among pediatric cyclists, many children continue to be injured while riding without a helmet. To determine where programs and policies intended to promote helmet use should be directed, we surveyed a large national dataset to identify variables asso...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2015.02.025
更新日期：2015-08-01 00:00:00
abstract：:From 1979 to 1990, 33 children required operative treatment for pancreatitis. Causes included ductal abnormalities (12 children), trauma (10 children), idiopathic (four children), gallstones (three children), drug-induced (three children), and tumor (one child). This study reviews the characteristics and outcome of th...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:From 1965 to 1985, of 1108 patients with advanced gastric cancer who underwent gastric resection in our department, 216 patients (19.5%) had synchronous peritoneal dissemination (PD) or liver metastasis (LM). The 1-year survival rate was 22.5% for PD, 14.7% for LM, and 4.8% for PD plus LM (p less than 0.01). With PD, ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:Anomalies of the appendix are extremely rare. The case presented here has not been previously described and is that of a "horseshoe" appendix in a 33-year-old man with sigmoid diverticulitis. This appendiceal variant cannot be classified into any of the existing categories of appendiceal anomalies. ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Diffuse compensatory extramedullary hematopoiesis is well known to involve the spleen and liver in certain hematologic disorders. Hematopoietic tumor masses occasionally form and usually occur paraspinally in the posterior mediastinum. A 31-year-old patient with thalassemia is described with a massive, solitary, intra...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：BACKGROUND:Differentiation between parathyroid hyperplasia and adenoma is difficult and based on the surgeon's skill. Microarrays and other sophisticated research tools generate information about differential gene expression in various tissues. Exploration of genes that express differentially in 1 tissue will enable id...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.2002.128614
更新日期：2002-12-01 00:00:00
abstract：:This study compares the National Board of Medical Examiners Part II (NBME II) subtest scores of medical students attaining honors in a clinical clerkship to those of students receiving a passing score to determine if the NBME II examinations could be considered independently as valid indicators of clinical achievement...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：BACKGROUND:Activated factor X (FXa) is involved in hemostasis, thrombogenesis, inflammation, and cellular immune response. Tissue factor (TF) is an initiator of blood coagulation. We investigated whether FXa induces TF expression in human peripheral monocytes and whether treatment with FXa inhibitor reduces TF expressi...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.2001.116452
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND:Patient-reported outcomes are measured in chronic disease states to inform intervention and management decisions while minimizing negative outcomes. We hypothesized that health-related quality of life in patients with multiple endocrine neoplasia type 2A would be worse than the general US population but simi...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2018.04.036
更新日期：2018-09-01 00:00:00
abstract：:An increase in the left ventricular end-diastolic pressure (LVEDP), resulting from the intravenous administration of blood, a colloid solution, or a crystalloid solution, was accompanied by an increase in both the inner:outer left ventricular wall flow ratio and the diastolic myocardial tissue pressure gradient. With ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:Plasma lipid and lipoprotein levels and composition of the very low-, low-, and high-density lipoproteins (VLDL, LDL, HDL) were studied in white New Zealand rabbits in the control state, 8 weeks after initiation of a 2% cholesterol-enriched diet, and sequentially for 16 weeks following a partial ileal bypass (PIB) pro...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：BACKGROUND:Anaplastic thyroid cancer is an aggressive and fatal malignancy. Many advanced cancers are characterized by glucose dependency, leading to oxidative stress and cellular proliferation. Therefore, we sought to determine if a low glucose environment (in vitro) or a ketogenic diet (in vivo) could inhibit anaplas...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2019.06.042
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:Despite the recommendations of the Advanced Trauma Life Support course of the American College of Surgeons, patients undergo computed tomography (CT) in local hospitals before transfer to a trauma center. The problem of repeat CTs caused by technical and protocol issues is ongoing. The objective is to measur...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2018.07.007
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:Early and accurate assessment of the response to neoadjuvant chemotherapy offers the potential to optimize treatment to obtain improved responses. We aimed to predict the response to neoadjuvant chemotherapy using a second breast core needle biopsy after a median of 2 cycles of neoadjuvant chemotherapy. MET...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2020.06.045
更新日期：2020-12-01 00:00:00
abstract：:We report four cases of abdominal aortic aneurysm associated with horseshoe kidney. These cases illustrate the factors that determine the ease of resection in this situation: whether the isthmus of the kidney is parenchymatous or thin and fibrous; the presence or absence of anomalous renal arteries; and the origin of ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1981-03-01 00:00:00
abstract：BACKGROUND:Parathyroid autotransplantation (PTHAT) during thyroidectomy has been shown to reduce the incidence of permanent hypoparathyroidism. Although selective PTHAT is most commonly adopted, the value of routine PTHAT has not been well documented. METHODS:From January, 1998 to March, 1999, an operative strategy in...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.2001.111125
更新日期：2001-03-01 00:00:00
abstract：:Multivisceral isografts and allografts were transplanted to Lewis rats, and the histopathologic changes were studied in the liver, intestine, and other constituent organs. Rats receiving isografts had indefinite survival with maintenance of weight. With multivisceral allografts (from Brown-Norway donors), the intestin...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:A series of 76 patients treated for carcinoma of the extrahepatic bile ducts at three Swedish hospitals during the years 1952 to 1973 was studied. The mean age of the patients was 68 years. Gallstones were found in 22 patients, whereas 11 patients had undergone cholecystectomy earlier for gallstone disease. At operati...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：BACKGROUND:Operative interventions have traditionally been seen as expensive; therefore, surgery has been given low priority in global health care planning in low-income countries. A growing body of evidence indicates that surgery can also be highly cost effective in low-income settings, but our current knowledge of th...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2015.01.026
更新日期：2015-06-01 00:00:00
abstract：:The ability of the lung to decrease blood flow to an atelectatic lobe and to increase flow to normal after reinflation was investigated with a model using left lower lobe atelectasis (LLLA) in the dog. The change in the shunt fraction QS/Qt with continuing LLLA was assumed to represent a change in blood flow to the LL...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：BACKGROUND:The risk of dying from primary hyperparathyroidism (pHPT) is controversial and has been explored mainly in single parathyroid gland disease. The present study investigates mortality in pHPT due to multiple parathyroid gland disease. METHODS:We used the nationwide Swedish In-patient Register and Cause-of-Dea...
journal_title：Surgery
pub_type： 杂志文章,多中心研究
doi：10.1016/j.surg.2004.05.044
更新日期：2004-11-01 00:00:00
abstract：BACKGROUND:To avoid the complications associated with overfeeding or underfeeding, the energy requirements of patients receiving total parenteral nutrition (TPN) must be accurately prescribed. However, until recently it has not been possible to directly measure the rates of total energy expenditure (TEE) in surgical pa...
journal_title：Surgery
pub_type： 临床试验,杂志文章
doi：10.1016/s0039-6060(05)80010-8
更新日期：1995-07-01 00:00:00
abstract：BACKGROUND:Coronary artery bypass grafting surgery has an increased risk of adverse events in obese patients. This increased risk might be explained in part by an increased intra-abdominal pressure and the development of intra-abdominal hypertension. Therefore, the objective of this study was to investigate the correla...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2019.05.038
更新日期：2019-12-01 00:00:00
abstract：BACKGROUND:Laparoscopic liver resection for liver metastases from colorectal cancer (CRLM) is performed in a relatively small number of institutions. Its operative results have been reported to be comparable with that of open laparotomy; however, information on its oncologic outcomes is scarce. This study aimed to comp...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2015.01.017
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND:Determination of the genetic composition of papillary thyroid cancers may help explain differences in observed clinical behavior. Comparative genomic hybridization (CGH) is a novel molecular cytogenetic assay that allows simultaneous detection of gains, losses, and amplification of genetic information, makin...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.2000.110847
更新日期：2000-12-01 00:00:00
abstract：:The recent surge in enthusiasm for laparoscopic surgery has created concern that abdominal insufflation with carbon dioxide produces a respiratory acidosis. This may be because of both transperitoneal gas absorption and impaired ventilation with increased dead space from elevated intraabdominal pressure. To examine th...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：INTRODUCTION:There is a paucity of data on outcomes for lung transplant (LT) recipients requiring general surgery procedures. This study examined outcomes after cholecystectomy in LT recipients using a large database. METHODS:The National Inpatient Sample Database (2005-2010) was queried for all LT patients requiring ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2015.02.021
更新日期：2015-08-01 00:00:00
abstract：BACKGROUND:Surgical enteral access prior to multimodality treatment for esophageal cancer is controversial as dysphagia is often used for feeding tube referral. We hypothesized that enteral access before neoadjuvant chemoradiation for esophageal cancer provides no benefit compared to that placed during definitive esoph...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2017.09.046
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND:Surgical attempts to treat obesity began because of the discouraging results of conservative medical treatment, which successfully achieved initial weight loss but failed to maintain it. Gastric restrictive procedures, currently the most popular surgical methods for obesity therapy, have proved to be effecti...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/s0039-6060(96)80111-5
更新日期：1996-03-01 00:00:00
