Abstract：
:The M07e megakaryoblastic leukemia cell line is strictly dependent on either interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for continuous growth. This study shows that recombinant human stem-cell factor (rhSCF) can completely replace these lymphokines in supporting the continued propagation of M07e cells mostly by eliciting GM-CSF secretion in this target. In fact, in short-term proliferation assays the stimulatory activity of SCF is blocked about 75% by a GM-CSF-specific serum. In addition, we could detect GM-CSF expression by SCF-stimulated M07e cells, both at the protein and mRNA levels. In contrast, SCF does not induce transcripts for any other cytokine to which M07e cells are responsive, including IL-2, IL-3, IL-4, and IL-6. Overall, these data show that the ability of SCF to support the growth of this megakaryocytic cell line is mediated mostly by the induction of an autocrine loop of activation involving GM-CSF production. The finding that SCF can stimulate GM-CSF secretion also in an IL-2-dependent T-lymphoblastic leukemia cell line indicates that SCF can act on cells of both myeloid and lymphoid lineages, and that the ability to induce cytokines in target cells represents an important aspect of its mechanism of action.
journal_name
Leukemiajournal_title
Leukemiaauthors
Kiss C,Cesano A,Zsebö KM,Clark SC,Santoli Dsubject
Has Abstractpub_date
1993-02-01 00:00:00pages
235-40issue
2eissn
0887-6924issn
1476-5551journal_volume
7pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract：:Dysplastic hematopoiesis is the morphological hallmark of myelodysplastic syndromes. Dysplastic features in one or more lineages are also found frequently in bone marrow aspirates from patients with de novo AML and have been associated with an unfavorable prognosis. We asked whether dyshematopoiesis is an independent ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) circulates in vivo as heterogeneous mixed populations (quasispecies). We analyzed the quasispecies nature of the first, second, and third hypervariable loops (V1, V2, and V3) of the gp 120 in plasma and viral isolates obtained from 19 infected individuals. Sequence analysis ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:Modern treatment strategies, consisting of intensive chemotherapy and cranial irradiation, have remarkably improved the prognosis for children with acute lymphoblastic leukemia. However, patients with a potential for cure are at risk of severe acute and late adverse effects of treatment. Furthermore, in 25-30% of pati...
journal_title：Leukemia
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1038/sj.leu.2402489
更新日期：2002-06-01 00:00:00
abstract：:The t(9;11)(p21;q23) has been associated with characteristic clinical features and a superior treatment outcome in previously untreated pediatric acute myeloblastic leukemia (AML), but has not been well studied in children with secondary AML. This translocation was detected in 6.7% of de novo and 46% of secondary AML ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:Mesenchymal stromal cells (MSCs) possess broad immunomodulatory capacities that are currently investigated for potential clinical application in treating autoimmune disorders. Third-party MSCs suppress alloantigen-induced proliferation of peripheral blood mononuclear cells providing the rationale for clinical use in g...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2014.273
更新日期：2014-12-01 00:00:00
abstract：:Successful treatment results for MLL-rearranged Acute Lymphoblastic Leukemia (ALL) in infants remain difficult to achieve. Significantly contributing to therapy failure is poor response to glucocorticoids (GCs), like prednisone. Thus, overcoming resistance to these drugs may be a crucial step towards improving prognos...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2013.245
更新日期：2014-04-01 00:00:00
abstract：:We have cloned two genes for cell surface molecules, capable of delivering the intracellular signals, which are modulated for their expression by Tax. One is the gamma chain of the interleukin-2 (IL-2) receptor which is suggested to be critical for IL-2-dependent growth of human T-cell leukemia virus type I (HTLV-I) i...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:The clinical and cell growth characteristics of 11 children with monosomy 7 presenting as preleukemia (eight cases) or acute nonlymphoblastic leukemia (three cases) were studied. Anemia was common to all patients, with nine showing leukocytosis, seven thrombocytopenia, and one thrombocytosis. There was a striking pred...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:By combining the polymerase chain reaction and differential library screening, a cDNA for an mRNA expressed in chicken avian myeloblastosis virus (AMV)-transformed monoblasts was isolated. This mRNA is not expressed in erythroblast or T-lymphoblast cell lines. Induced differentiation of the cells of the AMV-transforme...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Despite considerable advances in the treatment of multiple myeloma (MM) in the last decade, a substantial proportion of patients do not respond to current therapies or have a short duration of response. Furthermore, these treatments can have notable morbidity and are not uniformly tolerated in all patients. As there i...
journal_title：Leukemia
pub_type： 杂志文章,评审
doi：10.1038/s41375-020-0734-z
更新日期：2020-04-01 00:00:00
abstract：:The major cause of early death in acute promyelocytic leukemia (APL), the high risk of a bleeding diathesis is now successfully counteracted within a few days by differentiation therapy using ATRA. Moreover, no resistance to this drug has been recorded during induction when the usual presence of PML/RAR alpha was conf...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Bone marrow cells from most patients with acute promyelocytic leukemia contain a highly specific cytogenetic rearrangement, a reciprocal translocation between the long arms of chromosomes 15 and 17. Several cases of variant translocations involving 17q but not 15q have been reported, leading to the suggestion that the...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:This review will summarize the rationale for pursuing investigations into the use of retinoids for pediatric patients with cancer, describe the phase I results of all-trans-retinoic acid (ATRA) in children, and discuss the results of a series of preclinical and clinical pharmacokinetic studies of ATRA. The prognosis f...
journal_title：Leukemia
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The concept of arming antibodies with bioactive payloads for a site-specific therapy of cancer has gained considerable interest in recent years. However, a successful antibody-based targeting approach critically relies on the availability of a tumor-associated target that is not only preferentially expressed in the tu...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2017.208
更新日期：2018-02-01 00:00:00
abstract：:Exponentially growing human lymphoblasts (culture LS-2) were separated by cell sorting (FACS II, Becton Dickinson) according to their deoxyribonucleic acid (DNA) content, designating them at particular phases of the cell cycle. Prior to cell sorting the DNA has been fluorochrome-labeled with the Hoechst stain H 33342....
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:The long-term results in 130 patients with newly diagnosed acute myelogenous leukemia treated with continuous infusion high-dose ara-C (1.5 gm/m2/day x 4 days, CIHDAC) were compared with those in 264 patients treated in previous studies with standard dose ara-C (70-90 mg/m2/d x 7 days) plus either adriamycin (Ad-OAP),...
journal_title：Leukemia
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:Transcriptional regulation of the HOXA genes is thought to involve CTCF-mediated chromatin loops and the opposing actions of the COMPASS and Polycomb epigenetic complexes. We investigated the role of these mechanisms at the HOXA cluster in AML cells with the common NPM1c mutation, which express both HOXA and HOXB gene...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/s41375-020-0856-3
更新日期：2020-05-12 00:00:00
abstract：:CD13/aminopeptidase N (APN) is a cell surface metallopeptidase expressed by normal and leukemic myeloid cells, and by lymphoblasts in 5-10% of acute lymphoid leukemia (ALL) cases, previously classified as 'biphenotypic' or 'mixed-lineage' leukemias. In fresh cells from two early B-lineage, t(9;22)-positive, ALL cases ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Therapy for multiple myeloma (MM) has markedly changed in the past decade with the introduction of new drugs, but it is not clear whether the improvements have been sustained. We studied 1038 patients diagnosed between 2001 and 2010, grouping patients into two 5-year periods by diagnosis, 2001-2005 and 2006-2010. The ...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2013.313
更新日期：2014-05-01 00:00:00
abstract：:Recent molecular-genetic studies have revealed that in the majority of patients with secondary leukemia induced by topoisomerase II (topo II) inhibitors and also with infantile acute leukemia (IAL), the breakpoints are clustered within scaffold attachment regions (SARs) of 3'-MLL-bcr near exon 9. Genistein, abundant i...
journal_title：Leukemia
pub_type： 社论
doi：10.1038/sj.leu.2401344
更新日期：1999-03-01 00:00:00
abstract：:Gain of chromosome 18q and translocation t(14;18) are] frequently found in B-cell non-Hodgkin's lymphomas (B-NHL). Increased BCL2 transcription and BCL2 protein expression have been suggested to be the result of the gain. We utilized FISH, PCR and array CGH to study BCL2 and chromosome 18 copy number changes and rearr...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2403954
更新日期：2005-12-01 00:00:00
abstract：:The occurrence of leukemia in a gene therapy trial for SCID-X1 has highlighted insertional mutagenesis as an adverse effect. Although retroviral integration near the T-cell acute lymphoblastic leukemia (T-ALL) oncogene LIM-only protein 2 (LMO2) appears to be a common event, it is unclear why LMO2 was preferentially ta...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2404563
更新日期：2007-04-01 00:00:00
abstract：:The tie receptor tyrosine kinase mRNA was originally identified as an amplified product in reverse transcription-polymerase chain reaction analysis of human K562 leukemia cell RNA. In situ hybridization analysis revealed that the corresponding mouse gene is expressed predominantly in endothelial cells. We have explore...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:The molecular basis for autonomous growth of malignant forms of human T lymphocytes is not known. It can be investigated by functional responses of malignant cells in comparison with untransformed counterparts. At least two pathways (the CD2 and CD3 pathways) of human T-cell activation have been recently defined on th...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:Immunotherapy with T cell modified with gamma-retroviral or lentiviral (LV) vectors to express a chimeric antigen receptor (CAR) has shown remarkable efficacy in clinical trials. However, the potential for insertional mutagenesis and genotoxicity of viral vectors is a safety concern, and their cost and regulatory dema...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2016.180
更新日期：2017-01-01 00:00:00
abstract：:Imatinib (STI571, Gleevec/Glivec) is a potent selective tyrosine kinase inhibitor and is used successfully in the treatment of chronic myeloid leukemia (CML). While karyotype alterations, in addition to the Philadelphia chromosome, are a common phenomenon of progressing CML, the observation of BCR-ABL-negative leukemi...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2403861
更新日期：2005-09-01 00:00:00
abstract：:p53 is frequently wild type (wt) in diffuse large B-cell lymphoma (DLBCL) associated with t(14;18)(q32;q21) that overexpresses BCL2. Nutlin-3a is a small molecule that activates the p53 pathway by disrupting p53-MDM2 interaction. We show that nutlin-3a activates p53 in DLBCL cells associated with t(14;18)(q32;q21), BC...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2011.28
更新日期：2011-05-01 00:00:00
abstract：:Promising new drugs are being evaluated for treatment of multiple myeloma (MM), but their impact should be measured against the expected outcome in patients failing current therapies. However, the natural history of relapsed disease in the current era remains unclear. We studied 286 patients with relapsed MM, who were...
journal_title：Leukemia
pub_type： 杂志文章,多中心研究
doi：10.1038/leu.2011.196
更新日期：2012-01-01 00:00:00
abstract：:Acute myeloid leukemia (AML) involves a block in terminal differentiation of the myeloid lineage and uncontrolled proliferation of a progenitor state. Using phorbol myristate acetate (PMA), it is possible to overcome this block in THP-1 cells (an M5-AML containing the MLL-MLLT3 fusion), resulting in differentiation to...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2009.246
更新日期：2010-02-01 00:00:00
abstract：:Long-term disease-free survival following conventional cytotoxic therapy is extremely rare in patients with advanced-stage mantle cell lymphoma (MCL). High-dose conditioning therapy consisting of hyperfractionated total body irradiation (TBI, 14.4 Gy) and cyclophosphamide (200 mg/kg) was therefore offered to 13 patien...
journal_title：Leukemia
pub_type： 临床试验,杂志文章
doi：
更新日期：1996-12-01 00:00:00
