Abstract:
:The microsomal fraction of bone marrow contains cytochrome P-450 (39 +/- 11 pmoles/mg microsomal protein) and monooxygenase activity could be demonstrated by the O-dealkylation of 7-ethoxycoumarin (114 +/- 65 pmoles/(min X mg microsomal protein] and the hydroxylation of benzene to phenol (51 +/- 8.6 pmol/45 min X mg microsomal protein). This monooxygenase system differs from that in liver in various aspects. The conversion of benzene to phenol calculated as molecular activity was about 4 times higher than in liver and no induction by phenobarbital could be observed. Aroclor 1254 induced the cytochrome P-450 content about twofold but lowered the O-dealkylation activity of 7-ethoxycoumarin in contrast to liver. Pretreatment with benzene did not change the O-dealkylation in bone marrow, but had a stimulating effect on benzene monooxygenation and covalent binding of 14C-benzene metabolites. From these results we conclude that the bone marrow monooxygenase system develops its own pattern of cytochrome P-450 isoenzymes. Especially after chronic exposure to benzene this system can convert this chemical to phenol and secondary metabolites. The similar behaviour of phenol formation and covalent binding strengthens the hypothesis of a common pathway for metabolism and toxicity but the active intermediate still remains unknown.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Gollmer L,Graf H,Ullrich Vdoi
10.1016/0006-2952(84)90143-6subject
Has Abstractpub_date
1984-11-15 00:00:00pages
3597-602issue
22eissn
0006-2952issn
1873-2968pii
0006-2952(84)90143-6journal_volume
33pub_type
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pub_type: 杂志文章
doi:10.1016/j.bcp.2006.09.028
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pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1984-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2006-08-28 00:00:00
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pub_type: 传,历史文章,杂志文章,评审
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更新日期:2015-11-15 00:00:00
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pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00