Abstract：
:Fifty-two tetracycline-resistant Citrobacter spp. strains were isolated from farm-raised catfish. Morphological and biochemical characteristics indicated that 38 of the 52 citrobacters were Citrobacter freundii, 7 were C. amalonaticus and 7 were C. braakii. All isolates were resistant to multiple antibiotics. Polymerase chain reaction (PCR) protocols were developed to detect the presence of 3 tetracycline-resistance genes (tetA, tetB and tetG) from Citrobacter isolates. Oligonucleotide primers specifically targeting a 967-bp region of tetB successfully amplified the PCR amplicons from 3238 (85.0%) of C. freundii strains, 57 (71.0%) of C. amalonaticus and 47 (57%) from C. braakii. Oligonucleotide primers specific for the detection of tetA gene amplified the 417-bp PCR amplicons from 738 (18.0%) of tetracycline-resistant C. freundii only. The assay failed to amplify tetA genes from C. brakii or C. amalonaticus. Plasmids (2.0-16.0kb) were isolated from 14 of the 38 strains of C. freundii. Strains of C. amalonaticus and C. brakii did not contain any plasmids. Dendrogram analysis of the SpeI pulsed field gel electrophoresis (PFGE) results identified 23 distinct macrorestriction patterns (mrps) among the 36 strains of C. freundii, 3 distinct mrps among the 7 strains of C. braakii and 4 unique mrps among the 7 strains of C. amalonaticus. Our results indicate that citrobacters from catfish could serve as reservoirs of tetracycline-resistance determinants.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
Nawaz M,Khan AA,Khan S,Sung K,Steele Rdoi
10.1016/j.fm.2007.07.008subject
Has Abstractpub_date
2008-02-01 00:00:00pages
85-91issue
1eissn
0740-0020issn
1095-9998pii
S0740-0020(07)00092-5journal_volume
25pub_type
杂志文章abstract：:The growth of spoiling yeasts in beverages results in reduced quality, economic and image losses. Therefore, biochemical and DNA-based identification methods have been developed but are mostly time-consuming and laborious. Matrix-Assisted-Laser-Desorption/Ionization-Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) coul...
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doi：10.1016/j.fm.2014.05.007
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Food microbiology
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：Food microbiology
pub_type： 杂志文章
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更新日期：2009-06-01 00:00:00
abstract：:This study determined the ultraviolet-C (UV-C) dose necessary to reduce 90% population (DUV-C) of 17 spoilage yeasts and their composited inoculum in orange juice (pH 3.71, 11.60 °Brix, 0.55% citric acid, 2.46% w/v insoluble solids). Growth parameters of all test yeasts were first established to standardize the growth...
journal_title：Food microbiology
pub_type： 杂志文章
doi：10.1016/j.fm.2018.10.003
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