Carbohydrate mobilization and gene regulatory profile in the pseudobulb of Oncidium orchid during the flowering process.


:The pseudobulb of Oncidium orchid is a storage organ for supplying water, minerals and carbohydrates to the developing inflorescence. Different patterns of mannan, starch and pectin metabolism were observed in the pseudobulb of three developmental stages by histochemical staining and high performance anion exchange chromatographic (HPAEC) analysis. Copious pectin was strongly stained by ruthenium red in young pseudobulbs demonstrating that mannan and pectin were preferentially accumulated in the young pseudobulb sink at inflorescence pre-initiation stage. Concomitant with the emergence of the inflorescence, mannan and pectin decreased gradually and converted to starch. The starch, synthesized at the inflorescence developing stage, was eventually degraded at the floral development stage. A systematic survey on the subtractive EST (expression sequence tag) library of pseudobulb in the inflorescence pre-initiation stage revealed the presence of five groups of gene homologues related to sucrose, mannan, starch, pectin and other carbohydrate metabolism. The transcriptional level of 13 relevant genes related to carbohydrate metabolism was characterized from pseudobulbs of three different developmental stages. The specific activities of the enzymes encoded by these genes were also assayed. The expression profiles of these genes show that the transcriptional levels largely correlated with the enzyme activities, which were associated with the respective carbohydrate pools. These results demonstrated a novel functional profile of polysaccharide mobilization pathway as well as their relevant gene expression in the pseudobulb of Oncidium orchid during the flowering process.






Wang CY,Chiou CY,Wang HL,Krishnamurthy R,Venkatagiri S,Tan J,Yeh KW




Has Abstract


2008-04-01 00:00:00














  • Ultrastructure of fusion products from soybean cell culture and sweet clover leaf protoplasts.

    abstract::Protoplasts from cultured cells of soybean (Glycine max L.) and from sweet clover (Melilotus officinalis L.) mesophyll cells were fused with polyethylene glycol and subsequently cultured for six days. The resulting fusion products as well as unfused protoplasts of each parental species regenerated cell walls and divid...


    pub_type: 杂志文章


    authors: Fowke LC,Rennie PJ,Kirkpatrick JW,Constabel F

    更新日期:1976-01-01 00:00:00

  • Purification and properties of an endo-beta-1,4-glucanase from strawberry and down-regulation of the corresponding gene, cel1.

    abstract::An endo-beta-1,4-glucanase (EG) was purified from ripe strawberry (Fragaria x ananassa Duch.) fruit using cellulose affinity chromatography. The purified enzyme gave a single protein band of 54 kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of this protein showed ...


    pub_type: 杂志文章


    authors: Woolley LC,James DJ,Manning K

    更新日期:2001-11-01 00:00:00

  • Organ-specific analysis of the anaerobic primary metabolism in rice and wheat seedlings. I: Dark ethanol production is dominated by the shoots.

    abstract::During anaerobiosis in darkness the main route for ATP production in plants is through glycolysis in combination with fermentation. We compared the organ-specific anaerobic fermentation of flooding-tolerant rice (Oryza sativa) and sensitive wheat (Triticum aestivum) seedlings. A sensitive laser-based photoacoustic tra...


    pub_type: 杂志文章


    authors: Mustroph A,Boamfa EI,Laarhoven LJ,Harren FJ,Albrecht G,Grimm B

    更新日期:2006-12-01 00:00:00

  • OsBIMK1, a rice MAP kinase gene involved in disease resistance responses.

    abstract::The activation of mitogen-activated protein kinases (MAPKs) has been previously implicated in signal transduction during plant responses to pathogen attack as well as to various environmental stresses. We have isolated from rice a new MAPK cDNA, OsBIMK1 ( O ryza s ativa L. BTH-induced MAPK 1), which encodes a 369-amin...


    pub_type: 杂志文章


    authors: Song F,Goodman RM

    更新日期:2002-10-01 00:00:00

  • Osmotic relations during elongation growth in hypocotyls of Helianthus annum L.

    abstract::The relationship between growth, change in cell osmotic pressure and accumulation of osmotic solutes was investigated in hypocotyls of sunflower (Helianthus annum L.) seedlings. During growth in darkness the osmotic pressure decreased by 50% between days 2 and 6 after sowing. After irradiation of dark-grown seedlings ...


    pub_type: 杂志文章


    authors: Kutschera U

    更新日期:1991-04-01 00:00:00

  • Phytochrome-mediated bud development in Pisum sativum.

    abstract::Light-grown dwarf peas were disbudded except for a single lateral bud, then transferred to darkness at 24° C. During the dark period the seedlings were irradiated daily for 5 or 7 min with R or FR. The buds exposed to R developed into shoots faster than those irradiated with FR. The R effect was FR reversible, and the...


    pub_type: 杂志文章


    authors: Khudairi AK,Johnnykutty AT,Agarwal S

    更新日期:1971-06-01 00:00:00

  • An unusual effect of the far-red absorbing form of phytochrome: Photoinhibition of seed germination inBromus sterilis L.

    abstract::Seeds ofBromus sterilis L. germinated between 80-100% in darkness at 15° C but were inhibited by exposure to white or red light for 8 h per day. Exposure to far-red light resulted in germination similar to, or less than, that of seeds maintained in darkness. Germination is not permanently inhibited by light as seeds a...


    pub_type: 杂志文章


    authors: Hilton JR

    更新日期:1982-11-01 00:00:00

  • Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast.

    abstract::Nitric oxide (NO), an endogenous signaling molecule in animals and plants, mediates responses to abiotic and biotic stresses. Our previous work demonstrated that 100 microM sodium nitroprusside (SNP, an NO donor) treatment of maize seedlings increased K(+) accumulation in roots, leaves and sheathes, while decreasing N...


    pub_type: 杂志文章


    authors: Zhang Y,Wang L,Liu Y,Zhang Q,Wei Q,Zhang W

    更新日期:2006-08-01 00:00:00

  • Role of phloem in sucrose transport by Ricinus cotyledons.

    abstract::Sucrose is taken up and accumulated by cotyledons of Ricinus communis L. Autoradiographic studies reveal a predominant accumulation of sucrose in the phloem of the cotyledons. The export of sucrose from the cotyledons to hypocotyl and roots proceeds in the phloem by mass flow. These results, taken together with previo...


    pub_type: 杂志文章


    authors: Martin E,Komor E

    更新日期:1980-04-01 00:00:00

  • Glutamine synthetase in Scots pine seedlings and its control by blue light and light absorbed by phytochrome.

    abstract::The appearance of glutamine synthetase (GS. EC in response to light and nitrogen (NO 3 (-) , NH 4 (+) ) was studied in the organs (roots, hypocotyl, cotyledonary whorl) of the Scots pine (Pinus sylvestris L.) seedling. Although GS activity was found to be mainly (> 80%) located in the whorl where it increased...


    pub_type: 杂志文章


    authors: Elmlinger MW,Mohr H

    更新日期:1992-10-01 00:00:00

  • Hydraulic resistance to radial water flow in growing hypocotyl of soybean measured by a new pressure-perfusion technique.

    abstract::Hydraulic resistances to water flow have been determined in the cortex of hypocotyls of growing seedlings of soybean (Glycine max L. Merr. cv. Wayne). Data at the cell level (hydraulic conductivity, Lp; half-time of water exchange, T 1/2; elastic modulus, ɛ; diffusivity for the cell-to-cell pathway, D c) were obtained...


    pub_type: 杂志文章


    authors: Steudle E,Boyer JS

    更新日期:1985-05-01 00:00:00

  • The fine structure and development of the trabeculae and the trabecular ring in Selaginella kraussiana.

    abstract::In the shoot apex of Selaginella kraussiana A. Br., air spaces develop between the endodermal cells, isolating the two steles from the cortex. The endodermal cells elongate to form trabeculae connecting the two tissues. As each trabecular cell develops, cuticularization occurs on the external surface at a point mid-wa...


    pub_type: 杂志文章


    authors: McLean B,Juniper BE

    更新日期:1979-01-01 00:00:00

  • Towards the development of an oral vaccine against porcine cysticercosis: expression of the protective HP6/TSOL18 antigen in transgenic carrots cells.

    abstract:MAIN CONCLUSION:The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-...


    pub_type: 杂志文章


    authors: Monreal-Escalante E,Govea-Alonso DO,Hernández M,Cervantes J,Salazar-González JA,Romero-Maldonado A,Rosas G,Garate T,Fragoso G,Sciutto E,Rosales-Mendoza S

    更新日期:2016-03-01 00:00:00

  • Phytochrome-mediated growth responses in green and etiolated Lemna minor.

    abstract::The growth of Lemna minor in darkness is log-linear, at a much reduced rate compared to growth in white or red light. This rate of frond production in darkness is stimulated by kinetin, yeast extract, and thiamine either in green plants transferred directly from the light or in plants which had been grown in the dark ...


    pub_type: 杂志文章


    authors: Frick H,Mohr H

    更新日期:1972-09-01 00:00:00

  • The occurrence of caulonema-specific proteins in different moss species.

    abstract::Soluble proteins extracted with Tris-buffer pH 8.8 from different mosses are analysed by microgelelectrophoretic method for comparison to the specific proteins present only in the caulonema of Funaria hygrometrica. The protein patterns are also compared with those of liverwort, fern gametophytes and sporophytes and to...


    pub_type: 杂志文章


    authors: Sood S,Brenner K,Bopp M

    更新日期:1978-01-01 00:00:00

  • Changes in the phenolic metabolism of suspension cultures of Acer pseudoplatanus L. Caused by the addition of 2-(chloroethyl)phosphonic acid (CEPA).

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    pub_type: 杂志文章


    authors: Westcott RJ

    更新日期:1976-01-01 00:00:00

  • Conversion of gibberellin A20 to gibberellins A 1 and A 5 in a cell-free system from Phaseolus vulgaris.

    abstract::The soluble fraction of a cell-free system from immature seeds of Phaseolus vulgaris L. converts gibberellin A20 (GA20) to GA1 and GA5. It does however not metabolize GA1 and GA29 to GA5, showing that in this system GA20 is converted directly to GA5. The steps from GA20 to GA1 (3-hydroxylation) and from GA20 to GA5 (Δ...


    pub_type: 杂志文章


    authors: Kamiya Y,Takahashi M,Takahashi N,Graebe JE

    更新日期:1984-09-01 00:00:00

  • Some observations on the role of ATP in sieve tube translocation.

    abstract::Using the aphid stylet technique (14)C ATP was shown to be readily taken up into the sieve elements of willow. At the same time this compound was found to be metabolised during uptake resulting in labelled ADP and AMP appearing in the stylet exudate. Longitudinal movement of labelled ATP was also found to occur.Measur...


    pub_type: 杂志文章


    authors: Gardner DC,Peel AJ

    更新日期:1972-09-01 00:00:00

  • The influence of light quality on the phytochrome content of light grown Sinapis alba L. and Phaseolus aureus Roxb.

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    pub_type: 杂志文章


    authors: Kilsby CA,Johnson CB

    更新日期:1981-10-01 00:00:00

  • Subcellular localization of luteolin glucuronides and related enzymes in rye mesophyll.

    abstract::Vacuoles were isolated by osmotic rupture of mesophyll protoplasts from the primary leaves of 4-d- and 7-d-old plants of rye (Secale cereale L.). Their content of two flavones, luteolin 7-O-[β-D-glucuronosyl-(1→2)β-D-glucuronide] (R2) and luteolin 7-O-[β-D-glucuronosy 1 (1→2) β-D-glucuronide]-4'-O-β-D-glucuronide (R1)...


    pub_type: 杂志文章


    authors: Anhalt S,Weissenböck G

    更新日期:1992-04-01 00:00:00

  • Climate change increases the risk of herbicide-resistant weeds due to enhanced detoxification.

    abstract:MAIN CONCLUSION:Global warming will increase the incidence of metabolism-based reduced herbicide efficacy on weeds and, therefore, the risk for evolution of non-target site herbicide resistance. Climate changes affect food security both directly and indirectly. Weeds are the major biotic factor limiting crop production...


    pub_type: 杂志文章


    authors: Matzrafi M,Seiwert B,Reemtsma T,Rubin B,Peleg Z

    更新日期:2016-12-01 00:00:00

  • Three endo-β-mannanase genes expressed in the micropylar endosperm and in the radicle influence germination of Arabidopsis thaliana seeds.

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    pub_type: 杂志文章


    authors: Iglesias-Fernández R,Rodríguez-Gacio MC,Barrero-Sicilia C,Carbonero P,Matilla A

    更新日期:2011-01-01 00:00:00

  • The distribution of microtubules in differentiating cells of Micrasterias denticulata bréb.

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    pub_type: 杂志文章


    authors: Kiermayer O

    更新日期:1968-09-01 00:00:00

  • Assessment of nutrient remobilization through structural changes of palisade and spongy parenchyma in oilseed rape leaves during senescence.

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    pub_type: 杂志文章


    authors: Sorin C,Musse M,Mariette F,Bouchereau A,Leport L

    更新日期:2015-02-01 00:00:00

  • Intertribal hybrid plants produced from crossing Arabidopsis thaliana with apomictic Boechera.

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    pub_type: 杂志文章


    authors: Lohe AR,Perotti E

    更新日期:2012-08-01 00:00:00

  • Quantitative correlation of peripheral and intrinsic core polypeptides of photosystem II with photosynthetic electron-transport activity ofAcetabularia mediterranea in red and blue light.

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    pub_type: 杂志文章


    authors: Schmid R,Wennicke R,Fleischhauer S

    更新日期:1990-10-01 00:00:00

  • Aspartate kinase and the synthesis of aspartate-derived amino acids in wheat.

    abstract::Aspartate kinase (EC has been purified from 7 day etiolated wheat (Triticum aestivum L. var. Maris Freeman) seedlings and from embryos imbibed for 8 h. The enzyme was 50% inhibited by 0.25 mM lysine. In this study wheat aspartate kinase was not inhibited by threonine alone or cooperatively with lysine; these...


    pub_type: 杂志文章


    authors: Bright SW,Shewry PR,Miflin BJ

    更新日期:1978-01-01 00:00:00

  • Effect of chilling and gibberellic acid on growth potential of excised embryos of Ruellia humilis.

    abstract::Dormancy in intact seeds of Ruellia humilis is broken by chilling or by treatment with gibberellic acid (GA3). Embryos are nondormant and will grow when the seed coat is removed completely. Embryos from chilled or GA3-treated seeds have more growth potential than embryos from nontreated seeds. Dormancy is ascribed to ...


    pub_type: 杂志文章


    authors: Baskin JM,Baskin CC

    更新日期:1971-12-01 00:00:00

  • Sucrose storage in cell suspension cultures of Saccharum sp. (sugarcane) is regulated by a cycle of synthesis and degradation.

    abstract::We have investigated the regulation of sucrose storage in cell-suspension cultures of sugarcane. When grown in batch culture, sucrose accumulation commences after about 5 d, when the nitrogen supply is exhausted. Sucrose storage is also induced by decreasing the nitrogen supply to cells growing in a chemostat. The mea...


    pub_type: 杂志文章


    authors: Wendler R,Veith R,Dancer J,Stitt M,Komor E

    更新日期:1991-12-01 00:00:00

  • Photosensitized inhibitor formation in isolated, aging chloroplasts.

    abstract::The possible mechanism which leads to inactivation phenomena at high intensities of blue light during the color-sensitive phase in the time course of the Hill reaction was investigated in more detail. The following results were obtained: a) The inhibition of the Hill reaction is also observed during but not prior to t...


    pub_type: 杂志文章


    authors: Harnischfeger G

    更新日期:1972-12-01 00:00:00