当前位置： SCI文献检索 > PHARMACEUTICAL RESEARCH期刊下所有文献 > Designing 3D photopolymer hydrogels to regulate biomechanical cues and tissue growth for cartilage tissue engineering.

Designing 3D photopolymer hydrogels to regulate biomechanical cues and tissue growth for cartilage tissue engineering.

Abstract：

PURPOSE:Synthetic hydrogels fabricated from photopolymerization are attractive for tissue engineering for their controlled macroscopic properties, the ability to incorporate biological functionalities, and cell encapsulation. The goal of the present study was to exploit the attractive features of synthetic hydrogels to elucidate the role of gel structure and chemistry in regulating biomechanical cues. METHODS:Cartilage cells were encapsulated in poly(ethylene glycol) (PEG) hydrogels with different crosslinking densities. Cellular deformation was examined as a function of gel crosslinking. The effects of continuous versus intermittent dynamic loading regimens were examined. RGD, a cell adhesion peptide, was incorporated into PEG gels and subjected to mechanical loading. Chondrocyte morphology and activity was assessed by anabolic and catabolic ECM gene expression and matrix production by collagen and glycosaminoglycan production. RESULTS:Cell deformation was mediated by gel crosslinking. In the absence of loading, anabolic activity was moderately upregulated while catabolic activity was significantly inhibited regardless of gel crosslinking. Dynamic loading enhanced anabolic activities, but continuous loading inhibited catabolic activity, while intermittent loading stimulated catabolic activity. RGD acted as a mechanoreceptor to influence tissue deposition. CONCLUSIONS:We demonstrate the ability to regulate biomechanical cues through manipulations in the gel structure and chemistry and cartilage tissue engineering.

journal_name

Pharm Res

journal_title

Pharmaceutical research

authors

Bryant SJ,Nicodemus GD,Villanueva I

doi

10.1007/s11095-008-9619-y

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

2379-86

issue

10

eissn

0724-8741

issn

1573-904X

journal_volume

25

pub_type

杂志文章

相关文献

PHARMACEUTICAL RESEARCH文献大全
  • Effects of configuration around the chiral carbon atoms on the crystal properties of ephedrinium and pseudoephedrinium salicylates.

    abstract：:The physicochemical properties and crystal structures of the crystalline salts formed by the interaction of an achiral anion, salicylate, with homochiral and racemic ephedrinium and pseudoephedrinium cations were determined. The interaction of ephedrinium or pseudoephedrinium with salicylate in aqueous solution yielde...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1018945401484

    authors： Duddu SP,Grant DJ

    更新日期：1994-11-01 00:00:00

  • Nontoxic suramin as a chemosensitizer in patients: dosing nomogram development.

    abstract：PURPOSE:We reported that suramin produced chemosensitization at nontoxic doses. This benefit was lost at the approximately 10-fold higher, maximally tolerated doses (MTD). The aim of the current study was to identify in patients the chemosensitizing suramin dose that delivers 10-50 microM plasma concentrations over 48 ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-006-0165-1

    authors： Chen D,Song SH,Wientjes MG,Yeh TK,Zhao L,Villalona-Calero M,Otterson GA,Jensen R,Grever M,Murgo AJ,Au JL

    更新日期：2006-06-01 00:00:00

  • Sequence alignments of the H(+)-dependent oligopeptide transporter family PTR: inferences on structure and function of the intestinal PET1 transporter.

    abstract：PURPOSE:To study the structure and function of the intestinal H+/ peptide transporter PET1, we compared its amino acid sequence with those of related transporters belonging to the oligopeptide transporter family PTR, and with more distant transporter families. METHODS:We have developed a new approach to the sequence a...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1012070726480

    authors： Graul RC,Sadée W

    更新日期：1997-04-01 00:00:00

  • Structural effects on the binding of amine drugs with the diphenylmethyl functionality to cyclodextrins. II. A molecular modeling study.

    abstract：:Molecular modeling has been used to study the complexation between alpha, beta, or gamma-cyclodextrin (CD) and a group of amine compounds having the diphenylmethyl functionality. The computer program SYBYL 5.3 and the Tripos force field (version 5.2) were used for all the calculations. Three-dimensional structures of ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015816215751

    authors： Tong WQ,Lach JL,Chin TF,Guillory JK

    更新日期：1991-10-01 00:00:00

  • Antigene, ribozyme and aptamer nucleic acid drugs: progress and prospects.

    abstract：:Nucleic acids are increasingly being considered for therapeutic uses, either to interfere with the function of specific nucleic acids or to bind specific proteins. Three types of nucleic acid drugs are discussed in this review: aptamers, compounds which bind specific proteins; triplex forming (antigene) compounds; whi...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章,评审

    doi：10.1023/a:1016281324761

    authors： Stull RA,Szoka FC Jr

    更新日期：1995-04-01 00:00:00

  • Preparation and characterization of nanocapsules from preformed polymers by a new process based on emulsification-diffusion technique.

    abstract：PURPOSE:The aim of this study was to investigate whether biodegradable nanocapsules could be obtained by the emulsification-diffusion technique. METHODS:This technique consists of emulsifying an organic solution containing an oil, a polymer, and a drug in an aqueous solution of a stabilizing agent. The subsequent addi...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1011934328471

    authors： Quintanar-Guerrero D,Allémann E,Doelker E,Fessi H

    更新日期：1998-07-01 00:00:00

  • Salutary effects of two verapamil analogs in traumatic shock.

    abstract：:We studied the effects of two verapamil analogs, anipamil and ronipamil, in traumatic shock. Noble-Collip drum trauma produced a shock state characterized by an eight-fold increase in plasma cathepsin D activity, a 13-fold increase in the rate of plasma myocardial depressant factor (MDF) accumulation, and a survival t...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/A:1016363316777

    authors： Hock CE,Daitch JS,Lefer AM

    更新日期：1985-05-01 00:00:00

  • A physiologic model for simulating gastrointestinal flow and drug absorption in rats.

    abstract：PURPOSE:The development of a physiologically based absorption model for orally administered drugs in rats is described. METHODS:Unlike other models that use a multicompartmental approach, the GI tract is modeled as a continuous tube with spatially varying properties. The mass transport through the intestinal lumen is ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/b:pham.0000003373.72652.c0

    authors： Willmann S,Schmitt W,Keldenich J,Dressman JB

    更新日期：2003-11-01 00:00:00

  • An in vitro/in vivo correlation for the disintegration and onset of drug release from enteric-coated pellets.

    abstract：:An empirical mass-transfer model for enteric-coating dissolution that uses in vivo dissolution data to characterize the pH-dependent solubility properties of the polymer film and a mass-transfer coefficient determined from in vivo dissolution or disintegration studies is developed. Once the in vivo mass-transfer coeff...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1018986827350

    authors： Ebel JP,Jay M,Beihn RM

    更新日期：1993-02-01 00:00:00

  • Targeted delivery of complexes of biotin-PEG-polyethylenimine and NF-kappaB decoys to brain-derived endothelial cells in vitro.

    abstract：PURPOSE:To evaluate the effect of re-directing the uptake mechanism of polyplexes containing oligodeoxynucleotide (ODN) decoys to nuclear factor kappa B (NF-kappaB) from absorptive-mediated to receptor-mediated endocytosis. MATERIALS AND METHODS:Complexes of ODNs and a co-polymer of biotin-polyethylenglycol and polyet...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-007-9389-y

    authors： Bhattacharya R,Osburg B,Fischer D,Bickel U

    更新日期：2008-03-01 00:00:00

  • Bilateral Effects of Excipients on Protein Stability: Preferential Interaction Type of Excipient and Surface Aromatic Hydrophobicity of Protein.

    abstract：PURPOSE:Understanding the mechanism of protein-excipient interaction and illuminating the influencing factors on protein stability are key steps in the rational design of protein formulations. The objective of this study was to assess effects of preferential interaction type of excipient and surface aromatic hydrophobi...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-017-2152-0

    authors： Wen L,Zheng X,Wang X,Lan H,Yin Z

    更新日期：2017-07-01 00:00:00

  • Mechanistic investigation of drug release from asymmetric membrane tablets: effect of media gradients (osmotic pressure and concentration), and potential coating failures on in vitro release.

    abstract：PURPOSE:An asymmetric membrane (AM) tablet was developed for a soluble model compound to study the in vitro drug release mechanisms in challenge conditions, including osmotic gradients, concentration gradients, and under potential coating failure modes. Porous, semipermable membrane integrity may be compromised by a hi...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-006-9142-y

    authors： Am Ende MT,Miller LA

    更新日期：2007-02-01 00:00:00

  • The use of disaccharides in inhibiting enzymatic activity loss and secondary structure changes in freeze-dried β-galactosidase during storage.

    abstract：PURPOSE:The purpose of this study is to show how disaccharides differ in their ability to protect lyophilized β-galactosidase from enzymatic activity loss and secondary structure changes during storage. METHODS:β-galactosidase was lyophilized with trehalose, sucrose, cellobiose or melibiose at 2:1, 20:1 and 40:1 excip...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-010-0300-x

    authors： Heljo VP,Jouppila K,Hatanpää T,Juppo AM

    更新日期：2011-03-01 00:00:00

  • Controlled Endolysosomal Release of Agents by pH-responsive Polymer Blend Particles.

    abstract：PURPOSE:A key step of delivering extracellular agents to its intracellular target is to escape from endosomal/lysosomal compartments, while minimizing the release of digestive enzymes that may compromise cellular functions. In this study, we examined the intracellular distribution of both fluorecent cargoes and enzymes...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-015-1619-0

    authors： Zhan X,Tran KK,Wang L,Shen H

    更新日期：2015-07-01 00:00:00

  • Arachidonic acid-modified lovastatin discoidal reconstituted high density lipoprotein markedly decreases the drug leakage during the remodeling behaviors induced by lecithin cholesterol acyltransferase.

    abstract：PURPOSE:Our previous studies indicated that drug leaked from discoidal reconstituted high density lipoprotein (d-rHDL) during the remodeling behaviors induced by lecithin cholesterol acyl transferase (LCAT) abundant in circulation, thus decreasing the drug amount delivered into the target. In this study, arachidonic ac...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-013-1273-3

    authors： He H,Liu L,Bai H,Wang J,Zhang Y,Zhang W,Zhang M,Wu Z,Liu J

    更新日期：2014-07-01 00:00:00

  • The preparation and evaluation of a tablet dosage form of cyclosporine in dogs.

    abstract：:Cyclosporine (CsA) is commercially available for oral administration as a solution in olive oil with alcohol and an emulsifier. To improve its variable absorption and low patient acceptability, several oral formulations were prepared and tested in vitro and in vivo in dogs. A tablet formulation prepared by direct comp...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015815614959

    authors： Abdallah HY,Mayersohn M

    更新日期：1991-04-01 00:00:00

  • Investigation of the gastrointestinal transit and in vivo drug release of isosorbide-5-nitrate pellets.

    abstract：:An oral formulation of controlled-release isosorbide-5-nitrate pellets has been used to investigate the location of pellets in the gastrointestinal (GI) tract and, in parallel, to measure the drug absorption from these locations. Using the method of gamma scintigraphy the transit times and spreading of pellets in the ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016423404636

    authors： Fischer W,Boertz A,Davis SS,Khosla R,Cawello W,Sandrock K,Cordes G

    更新日期：1987-12-01 00:00:00

  • Colonic absorption of insulin-like growth factor I in vitro.

    abstract：:Colonic absorption of recombinant human insulin-like growth factor I (rhIGF-I) was measured in vitro using both rat and minipig colon. The permeability coefficients were 8.03 +/- 1.03 and 4.75 +/- 0.43 x 10(-8) cm sec-1 in the rat and minipig, respectively. The steady-state flux in rat colon was linearly related to th...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1018999106684

    authors： Quadros E,Landzert NM,LeRoy S,Gasparini F,Worosila G

    更新日期：1994-02-01 00:00:00

  • Formulation and performance of danazol nano-crystalline suspensions and spray dried powders.

    abstract：PURPOSE:This study focuses on the formulation optimization, in vitro and in vivo performance of differently sized nano-crystalline liquid suspensions and spray-dried powders of a poorly soluble BCS class II compound i.e. Danazol. METHODS:A DoE approach was utilized to optimize stabilizer concentration and formulate da...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-014-1567-0

    authors： Kumar S,Jog R,Shen J,Zolnik B,Sadrieh N,Burgess DJ

    更新日期：2015-05-01 00:00:00

  • The role of polysorbate 80 and HPβCD at the air-water interface of IgG solutions.

    abstract：PURPOSE:To test the hypothesis of surface displacement as the underlying mechanism for IgG stabilization by polysorbates and HPβCD against surface-induced aggregation. METHODS:Adsorption/desorption-kinetics of IgG-polysorbate 80/-HPβCD were monitored. Maximum bubble pressure method was used for processes within second...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-012-0854-x

    authors： Serno T,Härtl E,Besheer A,Miller R,Winter G

    更新日期：2013-01-01 00:00:00

  • Sensitivity of empirical metrics of rate of absorption in bioequivalence studies.

    abstract：PURPOSE:The sensitivity and effectiveness of indirect metrics proposed for the assessment of comparative absorption rates in bioequivalence studies [Cmax, Tmax, partial AUC (AUCp), feathered slope (SLf), intercept metric (I)] were originally tested by assuming first-order absorption. The present study re-evaluates thei...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1007521016985

    authors： Ring A,Tothfalusi L,Endrenyi L,Weiss M

    更新日期：2000-05-01 00:00:00

  • Comparative study of the skin penetration of protein transduction domains and a conjugated peptide.

    abstract：PURPOSE:We examined the ability of a protein transduction domain (PTD), YARA, to penetrate in the skin and carry a conjugated peptide, P20. The results with YARA were compared to those of a well-known PTD (TAT) and a control, nontransducing peptide (YKAc). The combined action of PTDs and lipid penetration enhancers was...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-005-2591-x

    authors： Lopes LB,Brophy CM,Furnish E,Flynn CR,Sparks O,Komalavilas P,Joshi L,Panitch A,Bentley MV

    更新日期：2005-05-01 00:00:00

  • pGlu-L-Dopa-Pro: a tripeptide prodrug targeting the intestinal peptide transporter for absorption and tissue enzymes for conversion.

    abstract：PURPOSE:The purpose of this study is to investigate the characteristics of pGlu-L-Dopa-Pro as a prodrug of L-Dopa. METHODS:pGlu-L-Dopa-Pro and L-Dopa-Pro were synthesized using the standard procedures of peptide synthesis. The conversion of pGlu-L-Dopa-Pro to L-Dopa was studied using pyroglutamyl aminopeptidase I and ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016239321494

    authors： Bai JP

    更新日期：1995-07-01 00:00:00

  • Interactions of phosphodiester and phosphorothioate oligonucleotides with intestinal epithelial Caco-2 cells.

    abstract：PURPOSE:Oral bioavailability for antisense oligonucleotides has recently been reported but the mechanistic details are not known. The proposed oral delivery of nucleic acids will, therefore, require an understanding of the membrane binding interactions, cell uptake and transport of oligonucleotides across the human gas...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016002606705

    authors： Beck GF,Irwin WJ,Nicklin PL,Akhtar S

    更新日期：1996-07-01 00:00:00

  • Determination of acrolein in urine by liquid chromatography and fluorescence detection of its quinoline derivative.

    abstract：:We describe an assay for acrolein in urine, employing derivatization with m-aminophenol in the presence of ferrous sulfate solution in sulfuric acid. The derivative (7-OH quinoline; DER) and the internal standard (quinine-bisulfate; IS) were separated on a 10-micron particle, 8 mm x 10-cm C18 cartridge in conjunction ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1018964401677

    authors： al-Rawithi S,el-Yazigi A,Nicholls PJ

    更新日期：1993-11-01 00:00:00

  • Solution behavior of PVP-VA and HPMC-AS-based amorphous solid dispersions and their bioavailability implications.

    abstract：PURPOSE:To identify the mechanism behind the unexpected bio-performance of two amorphous solid dispersions: BMS-A/PVP-VA and BMS-A/HPMC-AS. METHODS:Solubility of crystalline BMS-A in PVP-VA and HPMC-AS was measured by DSC. Drug-polymer interaction parameters were obtained by Flory-Huggins model fitting. Drug dissoluti...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-012-0695-7

    authors： Qian F,Wang J,Hartley R,Tao J,Haddadin R,Mathias N,Hussain M

    更新日期：2012-10-01 00:00:00

  • Pharmacokinetics of sulfasalazine metabolites in rats following concomitant oral administration of riboflavin.

    abstract：:Sulfasalazine, 60 mg/kg, was administered orally to groups of rats (n = 4) along with 1, 5, or 10 mg/kg of riboflavin. Plasma and urine were assayed for 5-aminosalicylic acid, acetyl-5-aminosalicylic acid, sulfapyridine, and acetyl-sulfapyridine using an HPLC method. The mean percent of dose recovered as total metabol...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015938706685

    authors： Chungi VS,Dittert LW,Shargel L

    更新日期：1989-12-01 00:00:00

  • Involvement of indoxyl sulfate in renal and central nervous system toxicities during cisplatin-induced acute renal failure.

    abstract：PURPOSE:The purpose of the present study was to explore the involvement of indoxyl sulfate (IS) in nephrotoxicity and central nervous system (CNS) toxicity in cisplatin (CDDP)-treated rats. MATERIALS AND METHODS:Renal function was evaluated by serum creatinine and BUN levels. The IS levels in the serum, brain and kidn...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-006-9183-2

    authors： Iwata K,Watanabe H,Morisaki T,Matsuzaki T,Ohmura T,Hamada A,Saito H

    更新日期：2007-04-01 00:00:00

  • Elucidation of Molecular Mechanisms Behind the Self-Assembly Behavior of Chitosan Amphiphilic Derivatives Through Experiment and Molecular Modeling.

    abstract：PURPOSE:Chitosan-based polymeric micelles (CBPMs) are considered as promising carriers for delivery of anticancer drugs, imaging agents and genes. To optimize the physicochemical, pharmaceutical and biological properties of CBPMs, the molecular mechanisms behind the self-assembly behavior of chitosan (CHI) amphiphilic ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-015-1750-y

    authors： Mahmoudzadeh M,Fassihi A,Dorkoosh F,Heshmatnejad R,Mahnam K,Sabzyan H,Sadeghi A

    更新日期：2015-12-01 00:00:00

  • The determinants of physician attitudes and subjective norms toward drug information sources: modification and test of the theory of reasoned action.

    abstract：PURPOSE:To improve upon the theory of reasoned action and apply it to pharmaceutical research, we investigated the effects of relevant appraisals attributes, and past behavior of physicians on the use of drug information sources. We also examined the moderating effects of practice characteristics. METHODS:A mail quest...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1012143915886

    authors： Gaither CA,Bagozzi RP,Ascione FJ,Kirking DM

    更新日期：1997-10-01 00:00:00