Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria.

Abstract:

:The rapid identification of the potentially toxigenic Corynebacterium species, C. diphtheriae, C. ulcerans and C. pseudotuberculosis is essential for diagnosis and treatment of diphtheria and diphtheria-like diseases. We used matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDIT-OF MS) in comparison with classical microbiological and molecular methods on 116 Corynebacterium strains. All 90 potentially toxigenic Corynebacterium strains collected by the German National Consiliary Laboratory on Diphtheria in a period of more than ten years were correctly identified by MALDI-TOF MS. We propose an algorithm for fast and reliable diagnosis of diphtheria incorporating MALDI-TOF MS, real-time tox PCR and Elek testing.

journal_name

Euro Surveill

authors

Konrad R,Berger A,Huber I,Boschert V,Hörmansdorfer S,Busch U,Hogardt M,Schubert S,Sing A

doi

10.2807/ese.15.43.19699-en

subject

Has Abstract

pub_date

2010-10-28 00:00:00

issue

43

eissn

1025-496X

issn

1560-7917

journal_volume

15

pub_type

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