Role of the endocannabinoid system on the antihyperalgesic action of gabapentin in animal model of neuropathic pain induced by partial sciatic nerve ligation.

Abstract:

:Gabapentin has antihyperalgesic action, decreasing central sensitization in neuropathic pain models; this effect depends on the mobilization of endogenous pain control pathways. This study aims to investigate the contribution of the endocannabinoid system to the antihyperalgesic action of gabapentin. Mus musculus Swiss, male, were submitted to PSL. On the 7th and 14th days post PSL, different groups were treated with CB1 receptor antagonist, AM281 via i.t. (2 μg/5 μl) or i.pl. (10 μg/20 μl) or CB2, AM630 via i.t. (5 μL i.t.) or (20 μL i.p.) and 15 min after gabapentin (30 mg / kg orally). Mechanical hyperalgesia was measured by the frequency of paw removal by the von Frey monofilament. Gabapentin demonstrated antihypernociceptive action, which was attenuated in animals pretreated with AM281 in both the i.t. and i.pl routes on the 7th and 14th days, differently from animals pretreated with AM630 that did not achieve a significant reduction with administration i.t. only on the 14th day with administration i.pl. The results show that endocannabinoid system contributes to the antihyperalgesic action of gabapetin in neuropathic pain by PSL, suggesting participation in the medullary and peripheral levels of CB1 receptors, and the peripheral performance of CB2 receptors.

journal_name

An Acad Bras Cienc

authors

Buffon AC,Javornik MA,Heymanns AC,Salm DC,Horewicz VV,Martins DF,Piovezan AP

doi

10.1590/0001-3765202020191155

subject

Has Abstract

pub_date

2020-12-14 00:00:00

pages

e20191155

issue

4

eissn

0001-3765

issn

1678-2690

pii

S0001-37652020000700939

journal_volume

92

pub_type

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