Abstract:
:Genome-editing tools such as Cre recombinase (Cre), zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and most recently the clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein system have revolutionized biomedical research, agriculture, microbial engineering, and therapeutic development. Direct delivery of genome editing enzymes, as opposed to their corresponding DNA and mRNA precursors, is advantageous since they do not require transcription and/or translation. In addition, prolonged overexpression is a problem when delivering viral vector or plasmid DNA which is bypassed when delivering whole proteins. This lowers the risk of insertional mutagenesis and makes for relatively easier manufacturing. However, a major limitation of utilizing genome editing proteins in vivo is their low delivery efficiency, and currently the most successful strategy involves using potentially immunogenic viral vectors. This lack of safe and effective non-viral delivery systems is still a big hurdle for the clinical translation of such enzymes. This review discusses the challenges of non-viral delivery strategies of widely used genome editing enzymes, including Cre recombinase, ZFNs and TALENs, CRISPR/Cas9, and Cas12a (Cpf1) in their protein format and highlights recent innovations of non-viral delivery strategies which have the potential to overcome current delivery limitations and advance the clinical translation of genome editing.
journal_name
Adv Drug Deliv Revjournal_title
Advanced drug delivery reviewsauthors
Li J,Røise JJ,He M,Das R,Murthy Ndoi
10.1016/j.addr.2020.09.004subject
Has Abstractpub_date
2021-01-01 00:00:00pages
99-117eissn
0169-409Xissn
1872-8294pii
S0169-409X(20)30128-9journal_volume
168pub_type
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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