Abstract:
:The stability of the Yersinia enterocolitica virulence plasmid (pYV) under different NaCl concentrations and under acidic pH conditions was investigated. Exposure of five strains representing five serotypes of pYV-bearing virulent Y. enterocolitica to 0.5, 2 and 5% NaCl and under conditions of pH 4, 5, and 6 for 24 h at 28 °C in the stationary phase did not lead to the loss of the virulent pYV from the surviving cells. At pH 3.0, the cells did not survive. Virulence assays using crystal violet binding, low-calcium response, Congo red-uptake, hydrophobicity by latex particle agglutination, and autoagglutination, as well as PCR assay to determine the presence of pYV indicated that the surviving cells were still virulent.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
Bhaduri Sdoi
10.1016/j.fm.2010.10.001subject
Has Abstractpub_date
2011-02-01 00:00:00pages
171-3issue
1eissn
0740-0020issn
1095-9998pii
S0740-0020(10)00250-9journal_volume
28pub_type
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