Abstract:
OBJECTIVE:To investigate whether fluorochrome-conjugated phalloidin can delineate cavernous smooth muscle (CSM) cells and whether it can be combined with immunofluorescence (IF) staining to quantify erectile dysfunction (ED)-associated changes. METHODS:ED was induced by cavernous nerve crush in rats. Penile tissues of control and ED rats were stained with Alexa-488-conjugated phalloidin and/or with antibodies against rat endothelial cell antigen (RECA), CD31, neuronal nitric oxide synthase (nNOS), and collagen-IV (Col-IV). RESULTS:Phalloidin was able to delineate CSM as composed of a circular and a longitudinal compartment. When combined with IF stain for CD31 or RECA, it helped the identification of the helicine arteries as covered by endothelial cells on both sides of the smooth muscle layer. When combined with IF stain for nNOS, it helped the identification that nNOS-positive nerves were primarily localized within the dorsal nerves and in the adventitia of dorsal arteries. When combined with IF stain for Col-IV, it helped identify that Col-IV was localized around smooth muscles and beneath the endothelium. Phalloidin also facilitated the quantitative analysis of ED-related changes in the penis. In rats with cavernous nerve injury, RECA or Col-IV expression did not change significantly, but CSM and nNOS nerve contents decreased significantly. CONCLUSION:Phalloidin stain improved penile histology, enabling the visualization of the circular and longitudinal compartments in the CSM. It also worked synergistically with IF stain, permitting the visualization of the dual endothelial covering in helicine arteries, and facilitating the quantification of ED-related histologic changes.
journal_name
Urologyjournal_title
Urologyauthors
Lin G,Qiu X,Fandel TM,Albersen M,Wang Z,Lue TF,Lin CSdoi
10.1016/j.urology.2011.06.021subject
Has Abstractpub_date
2011-10-01 00:00:00pages
970.e1-8issue
4eissn
0090-4295issn
1527-9995pii
S0090-4295(11)00635-2journal_volume
78pub_type
杂志文章相关文献
UROLOGY文献大全abstract::Urologic evaluation of a ninety-two-year-old man with hematuria suggested lower segmental renal infarction on selective renal angiogram. Nonvisualization of the lower segmental venous tributaries and capillaries on renal venogram was consistent with the operative findings of microscopic infiltrating transitional cell ...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(81)90429-5
更新日期:1981-08-01 00:00:00
abstract:OBJECTIVES:To investigate the long-term outcome of an organ-saving approach for renal cell carcinoma (RCC) with a normal opposite kidney (elective indication). METHODS:Since 1979, 180 patients have undergone nephron-sparing surgery at our institution for RCC in the presence of a normal contralateral kidney. The mean a...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/s0090-4295(00)00656-7
更新日期:2000-09-01 00:00:00
abstract::The management of 10 cases of penile gunshot wounds treated at Henry Ford Hospital from 1982 to 1986 is reviewed. All patients were assaulted by low velocity weapons (handguns). Eight patients had associated injuries, predominantly to the genital region (thigh, pubis, and scrotum). There were 5 urethral injuries; 4 we...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(90)80237-h
更新日期:1990-10-01 00:00:00
abstract::The Cavitron Ultrasonic Surgical Aspirator is a unique modality for precise tissue removal which results in increased visibility, reduced bleeding, and shorter operating time when dealing with vascular lesions or organs. Herein, we present our experience in a dog laboratory as well as in a clinical setting with this n...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/s0090-4295(84)80004-7
更新日期:1984-05-01 00:00:00
abstract:OBJECTIVES:To examine the predictive value of ultrasonic measurements obtained by transrectal ultrasonography for infravesical obstruction as evaluated by pressure flow studies. METHODS:In 85 men with moderate to severe lower urinary tract symptoms, ultrasonic measurements including prostatic volume, transition zone v...
journal_title:Urology
pub_type: 临床试验,杂志文章
doi:10.1016/S0090-4295(97)00310-5
更新日期:1997-10-01 00:00:00
abstract::Studies to detect ultrastructural changes in testicular tissues after a fifteen-minute exposure to ultrasonic waves were done in 10 men undergoing bilateral orchiectomies for prostatic carcinoma. Examination of Sertoli cells and germ cells in different stages of differentiation failed to reveal significant differences...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(87)90232-9
更新日期:1987-09-01 00:00:00
abstract:OBJECTIVES:To investigate the role of phospholipase Cε (PLCε) by silencing PLCε with short hairpin RNA (shRNA) in human bladder cancer cells BIU-87 in vitro and in vivo. METHODS:A PLCε shRNA expression vector was transfected into BIU-87 cells, and the expression of PLCε protein was detected by Western blotting. Cell p...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2011.03.014
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVES:To investigate the weak correlation between bladder outlet obstruction (BOO), as diagnosed using the provisional International Continence Society nomogram for the definition of BOO in men, and postvoid residual urine volume. METHODS:The relationship between voiding to completion and several indexes for blad...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/s0090-4295(03)00575-2
更新日期:2003-10-01 00:00:00
abstract:OBJECTIVES:To evaluate the effectiveness and morbidity of salvage brachytherapy for locally recurrent or persistent prostate cancer after radiotherapy failure. METHODS:In this retrospective study, 49 patients of median age 73.3 years (range 52.9 to 86.9) with biopsy-proven localized prostate cancer underwent interacti...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/s0090-4295(98)00492-0
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVES:In many institutions, cystography is obtained routinely after radical retropubic prostatectomy to assess the integrity of the vesicourethral anastomosis before the removal of the catheter. This study presents the Indiana experience with cystograms performed following radical retropubic prostatectomy. METHOD...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/S0090-4295(99)80163-0
更新日期:1995-07-01 00:00:00
abstract:OBJECTIVE:To describe the different ultrasound (US) findings of mature testicular hamartomas in children. MATERIALS AND METHODS:This is a retrospective study from January 2000 to July 2012 that reviewed the clinical, laboratory, and US findings of 7 children with a pathologic diagnosis of mature teratoma of the testis...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2013.07.046
更新日期:2014-01-01 00:00:00
abstract::An eight-year-old girl has had urge incontinence for the last three years and muscle weakness and pain in the lower extremities for one month. Urodynamic study revealed a bladder instability of severe degree. Tethered cord syndrome caused by a tight filum terminale was identified by metrizamide myelography. An untethe...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(92)90513-v
更新日期:1992-08-01 00:00:00
abstract:OBJECTIVE:To determine the relationship between obesity and febrile urinary tract infection (UTI) in young children. METHODS:We reviewed all medical records of children aged <3 years who visited our institution for febrile UTIs and infant national health checkups (controls) between January 2008 and February 2012. All ...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2014.03.032
更新日期:2014-08-01 00:00:00
abstract::A new operation, very simple to perform under local anesthesia, is described for urinary incontinence caused by operations on the prostate gland or the bladder neck; satisfactory results have been obtained, with no complications to date. The surgical procedure consists essentially of plication of the external urethral...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(75)90026-6
更新日期:1975-02-01 00:00:00
abstract::Renal cell carcinoma is a common entity often managed surgically with excellent survival benefits. We report a rare case of sarcomatoid renal cell carcinoma with aggressive growth kinetics after palliative resection captured radiographically. ...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2016.03.027
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVES:To review our experience with enterovesical fistulas in order to determine the most accurate diagnostic studies and most effective method of treatment. METHODS:A retrospective record review of 76 patients who were diagnosed and treated for enterovesical fistulas over a 12-year period was performed. Data col...
journal_title:Urology
pub_type: 临床试验,杂志文章
doi:10.1016/s0090-4295(94)80200-9
更新日期:1994-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the time to stone-free status after shock wave lithotripsy (SWL) for ureteral stones. METHODS:Medical records of 387 patients with ureteral stones who have been treated were retrospectively reviewed. Exclusion criteria for this analysis included nonopaque stones, prior ureteric surgery, multiple ...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2010.10.060
更新日期:2011-07-01 00:00:00
abstract::The experience gained from the implantation of 763 Flexirod Prosthesis is described. Special features of this design include a tapered distal tip which provides for better glans stability, a soft hinge which allows for improved penile concealment, and a trimmable tail which, together with preoperative measurement of t...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(84)90247-4
更新日期:1984-05-01 00:00:00
abstract::In 1981, the National Prostatic Cancer Treatment Group began an open survey of available A1, B1 prostatic adenocarcinomas to determine outcome and clinical characteristics. Their report as of September, 1985, summarizes the outcome and important features of the survey. The majority of A1 cases had a low tumor grade I ...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(87)90065-3
更新日期:1987-03-01 00:00:00
abstract:OBJECTIVES:To compare surgical and functional results of both surgical approaches to endoscopic partial nephrectomy. It is currently performed either by the transperitoneal (t) or the retroperitoneal (r) approach. METHODS:This was a retrospective, matched-pair comparison of 105 patients who underwent either transperit...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2010.02.057
更新日期:2011-01-01 00:00:00
abstract::Xanthine calculi are uncommonly encountered stones. When they occur, they typically do so in association with inborn metabolic disorders such as hereditary xanthinuria or Lesch-Nyhan syndrome. They may also occur in association with states of profound hyperuricemia such as myeloproliferative disease after treatment wi...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2005.10.057
更新日期:2006-05-01 00:00:00
abstract:OBJECTIVES:To evaluate our experience with primary carcinomas of the male urethra and to analyze the impact of tumor variables and treatment on overall, disease-specific, local recurrence-free, and metastasis-free survival. METHODS:Between 1958 and 1996, we identified 46 men with primary carcinoma of the bulbar and an...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/s0090-4295(98)00659-1
更新日期:1999-06-01 00:00:00
abstract:OBJECTIVES:To determine the effect of the combination of intranasal desmopressin spray and diclofenac sodium suppository on acute renal colic and compare it with diclofenac sodium suppository alone. METHODS:A total of 150 patients aged 15-65 years referred to our hospital with acute renal colic were included in a doub...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2008.05.053
更新日期:2010-03-01 00:00:00
abstract::Circumcision is generally undertaken for 3 reasons; first, as an item of religious practice, typically neonatally, although occasionally transpubertally, as a rite of passage; second, as a prophylactic measure against future ailments (for the reduction in the risk of penile cancer, urinary tract infection, and sexuall...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2009.10.005
更新日期:2010-07-01 00:00:00
abstract::Two patients presented with synchronous Hodgkin's disease and testicular germ cell neoplasms. Both patients are in complete remission following treatment with multidrug chemotherapy and radiation therapy. Despite epidemiologic similarities between Hodgkin's disease and testicular cancer, only 13 cases of their associa...
journal_title:Urology
pub_type: 杂志文章,评审
doi:10.1016/0090-4295(91)80208-o
更新日期:1991-02-01 00:00:00
abstract::The anticholinergic and antispasmodic activity of atropine, propantheline, imipramine, and flavoxate were judged by each drug's ability to inhibit bethanechol chloride and barium chloride-induced canine detrusor contractions. In this in vitro model, atropine and propantheline are pure anticholinergic agents. Imipramin...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(77)90279-5
更新日期:1977-01-01 00:00:00
abstract:OBJECTIVE:To introduce our hybrid technique using a Satinsky clamp for right-sided hand-assisted laparoscopic donor nephrectomy (HALDN) and to compare its effectiveness and safety with those of standard left-sided HALDN. METHODS:We conducted a retrospective analysis of 253 patients, who underwent HALDN (51 right and 2...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2014.09.006
更新日期:2014-12-01 00:00:00
abstract::Mesoblastic nephroma is the most common solid renal neoplasm in the first few months of life. It has been recognized only recently as a distinct entity, having been classified previously as Wilms tumor. There are relatively few ultrasonic descriptions of this entity in the literature. We present an additional case fea...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/0090-4295(82)90590-8
更新日期:1982-02-01 00:00:00
abstract:OBJECTIVES:To elucidate the clinical significance of the HER-2 gene alterations in urine-exfoliated cells detected by fluorescence in situ hybridization (FISH) in patients with urothelial transitional cell carcinoma. METHODS:The relative increase of HER-2 (RI-HER2) and gain of chromosome 17 (G-17) in urine-exfoliated ...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2004.05.004
更新日期:2004-09-01 00:00:00
abstract:OBJECTIVES:Recent evidence indicates that ischemia and reperfusion (free radical generation) are major etiologic factors in the rabbit bladder subjected to partial outlet obstruction. The current investigation was designed to determine the time course of damage caused by reactive oxygen species (ROS) and reactive nitro...
journal_title:Urology
pub_type: 杂志文章
doi:10.1016/j.urology.2007.09.047
更新日期:2007-12-01 00:00:00