Abstract：
:GnRH has several direct actions on rat granulosa cells. Specific receptors for GnRH have been demonstrated on rat and human ovaries. Whether the porcine ovary has specific receptors for GnRH is still debated and the physiological actions of GnRH on porcine granulosa cells have not yet been clarified. Consequently, we have examined the actions of a GnRH agonist (GnRHa) on basal and LH stimulated progesterone secretion by porcine granulosa cells. GnRHa inhibited both basal and LH stimulated progesterone secretion by granulosa cells from medium (3-5 mm) and large (6-10 mm) antral follicles during 3 day incubations. LH stimulated progesterone secretion was more sensitive to inhibition than basal progesterone secretion. Studies on the time course for GnRHa inhibition of progesterone secretion indicated that the decrease in progesterone secretion occurred 48 to 72 hr after first exposure to GnRHa. Earlier inhibition occurred in only a fraction of the experiments. GnRHa did not have to be present during the time when inhibition occurred. Incubations of 2 days with GnRHa were just as effective as 3 day incubations at inhibiting progesterone secretion on day 3. Furthermore, a 30 min exposure to GnRHa on day 1 was just as inhibitory as a full 2 day incubation with GnRHa in inhibiting LH stimulated progesterone secretion on day 3. Incubation of the cells for 3 days prior to exposure of the cells to GnRHa did not alter the time course for GnRHa action. GnRHa did not alter the DNA content of the cultures in up to 6 day incubations or the number of viable cells attached to the wells in up to 3 day incubations.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Ledwitz-Rigby Fdoi
10.1016/0739-7240(90)90032-usubject
Has Abstractpub_date
1990-04-01 00:00:00pages
265-72issue
2eissn
0739-7240issn
1879-0054pii
0739-7240(90)90032-Ujournal_volume
7pub_type
杂志文章abstract：:The aim of this study was to differentiate canine adipose-derived mesenchymal stem cells (ADMSCs) into insulin-producing cells by using culture media with different compositions to determine the most efficient media. Stem cells isolated from the fat tissues close to the bitch uterus were distributed into 6 groups: (1)...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2020.106572
更新日期：2021-01-01 00:00:00
abstract：:The aim of the study and short review was to present evidence that growth hormone (GH), locally produced insulin-like growth factors (IGFs), and IGF-binding proteins (IGFBPs) may have an important role in the control of ovarian function. There is clear evidence for a distinct GH-receptor mRNA expression and protein pr...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0739-7240(99)00044-2
更新日期：1999-10-01 00:00:00
abstract：:Zymosan is a cell wall component of the yeast Saccharomyces cerevisiae and produces severe inflammatory responses in mammals. When zymosan is peripherally injected in mammals, it induces several behavioral and physiological changes including anorexia and hyperthermia. However, to our knowledge, behavioral and physiolo...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2020.106471
更新日期：2020-07-01 00:00:00
abstract：:In male goats, being in permanent visual contact with females in estrus does not prevent seasonal variation in certain endocrine hormone levels and sexual activities. In this study, we tested whether continuous and full contact with females in estrus prevented seasonal endocrinological variation in bucks. In 1 experim...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.04.006
更新日期：2019-10-01 00:00:00
abstract：:To determine if poor maternal nutrition alters growth, body composition, circulating growth factors, and expression of genes involved in the development of muscle and adipose of offspring, 24 Dorset and Shropshire ewes were fed either 100% (control fed), 60% (restricted fed), or 126% (over fed) of National Research Co...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2014.05.002
更新日期：2014-10-01 00:00:00
abstract：:The ability of gonadotropin-releasing hormone (GnRH) to synchronize ovulation and new follicular wave emergence before a "superovulatory Day 0" protocol was assessed in Santa Inês ewes. For estrus synchronization, a 60-mg medroxyprogesterone acetate sponge was inserted for 6 d. One day before sponge removal, 37.5-μg d...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2015.07.002
更新日期：2016-01-01 00:00:00
abstract：:Adrenocorticotropic hormone (ACTH) challenges are frequently performed in repetition when evaluating stress or welfare in animals. To our knowledge, the repeatability of ACTH challenges in cattle, although fundamental to further studies of this type, has not yet been the subject of research. Therefore, the objective o...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.03.001
更新日期：2020-07-01 00:00:00
abstract：:Mature Hereford cows (n = 28) were used to determine the effect of percentage body fat on secretion of LH and content of GnRH in the infundibular stalk-median eminence (ISME). Cows were fed to maintain, lose, or gain weight to achieve body condition scores (BCS; 1 = emaciated; 9 = obese) of 3 to 7. Then cows were fed ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(92)90010-u
更新日期：1992-01-01 00:00:00
abstract：:An increasing demand for calcium during pregnancy and lactation can result in both clinical and subclinical hypocalcemia during the early lactation period in several mammalian species, in particular the dairy cow. Serotonin (5-HT) was recently identified as a regulator of lactation and bone turnover. The purpose of th...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.01.005
更新日期：2013-05-01 00:00:00
abstract：:The objective of the present study was to evaluate changes in concentrations of free insulin-like growth factor (IGF)-I in follicular fluid (FFL) during follicle development in the mare. Mares (n = 14) were classified as either in the follicular phase (n = 8) or luteal phase (n = 6). Follicles (n = 92) were categorize...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2005.03.003
更新日期：2005-11-01 00:00:00
abstract：:This is a test-report of ghrelin levels in plasma and proventriculus, the glandular portion of the avian stomach, by using a specific radioimmunoassay for acylated ghrelin, as well as the expression of the ghrelin gene in the proventriculus after a 12-h fasting period followed by a 6-h feeding period with 6-day-old la...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2006.03.006
更新日期：2007-05-01 00:00:00
abstract：:Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0% (w/w). c...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(91)90020-k
更新日期：1991-10-01 00:00:00
abstract：:Ghrelin, the endogenous ligand for growth hormone secretagogue receptor 1a (GHS-R1a), stimulates food intake in mammals centrally and peripherally. In contrast, central injection of ghrelin inhibits feeding in neonatal chicks (Gallus gallus), which is thought to be mediated by the corticotrophin-releasing hormone (CRH...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.12.002
更新日期：2014-04-01 00:00:00
abstract：:IL-2 has analgesic effects in both central and peripheral nervous systems. There are two distinct domains in IL-2 molecule mediating immunologic and analgesic activity, respectively. The analgesic domain of IL-2 may be composed of the 44th Phe, 45th Tyr, 107th Tyr, and 117th Phe residues that are located closely in th...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0739-7240(98)00026-5
更新日期：1998-09-01 00:00:00
abstract：:The pituitary gland, occupying a central position in the hypothalamo-pituitary thyroidal axis, produces thyrotropin (TSH), which is known to stimulate the thyroid gland to synthetize and release its products, thyroid hormones. TSH is produced by a specific cell population in the pituitary, the so-called thyrotropes. T...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2005.02.008
更新日期：2005-07-01 00:00:00
abstract：:In pigs, luteolytic sensitivity to PGF-2α (=LS) is delayed until d 13 of the estrous cycle. While the control of LS is unknown, it is temporally associated with macrophage (MAC; which secretes tumor necrosis factor [TNF]-α) infiltration into the corpora lutea (CL), and previous studies have shown that TNF-α induces LS...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.106426
更新日期：2020-07-01 00:00:00
abstract：:The experiments were conducted to determine the effects of the administration of growth hormone-releasing peptide-2 (GHRP-2, also named KP102), both orally by gavage and in feed, on the release of growth hormone (GH) in swine and to investigate whether attenuation of the GH response occurs after short-term treatment w...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(00)00085-0
更新日期：2001-01-01 00:00:00
abstract：:Twelve pregnant Brahman cows were randomly assigned to one of two treatment groups: 1) transported in a stock trailer for 24.2 km, unloaded at a second farm and penned for 1 hr, and then returned to the original farm (TRANS, n = 6); or 2) walked through the handling facilities (SHAM, n = 6). Treatments were repeated a...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(96)00115-4
更新日期：1997-03-01 00:00:00
abstract：:Crossbred ovariectomized ewes were treated with steroid therapies determined previously to be adequate (progesterone-primed) or inadequate (unprimed) for embryonic development in order to determine actual serum concentrations of replaced steroid hormones achieved by such treatments and to identify secreted endometrial...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(93)90017-6
更新日期：1993-04-01 00:00:00
abstract：:The flash glucose monitoring system (FGMS) Freestyle Libre provides estimates of blood glucose by constantly measuring the glucose concentration of the interstitial fluid through a sensor inserted in the subcutaneous space. The aim of this study was to evaluate the applicability and accuracy of the FGMS in dogs with d...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2020.106525
更新日期：2021-01-01 00:00:00
abstract：:The three biologic activities most commonly associated with tumors that produce Humoral Hypercalcemia of Malignancy (HHM) include; 1) adenylate cyclase stimulating activity (PTH-like activity), 2) in vitro bone resorbing activity, and 3) transforming growth factor activity. The canine adenocarcinoma (CAC-8) model of H...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0739-7240(88)90022-7
更新日期：1988-01-01 00:00:00
abstract：:The peroxisome proliferator-activated receptors (PPARs) are the members of superfamily of nuclear hormone receptors. A great number of studies in rodent and human have shown that PPARs were involved in the lipids metabolism. The goal of the current study was to investigate the expression pattern of PPAR genes in vario...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2004.05.003
更新日期：2005-01-01 00:00:00
abstract：:In vitro release of progesterone (P4) and estrone (E1) by the porcine placenta was characterized at 12 days of gestation between d 20 and 110. Placental P4 rose linearly from d 25 to 40, plateaued between d 40 and 50, continued to increase to a peak concentration at d 100, then decreased sharply to d 110. Placental E1...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(90)90007-m
更新日期：1990-10-01 00:00:00
abstract：:Glucocorticoid (GC) action depends on GC plasma concentration, cellular GC receptor expression, and the pre-receptor hormone metabolism catalyzed by 11β-hydroxysteroid dehydrogenase (11β-HSD). 11β-Hydroxysteroid dehydrogenase exists in 2 isoforms; 11β-HSD1 converts inactive cortisone to cortisol, and 11β-HSD2 converts...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2010.06.003
更新日期：2010-11-01 00:00:00
abstract：:Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the eff...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.07.002
更新日期：2013-10-01 00:00:00
abstract：:In ruminants, both milking frequency and exogenous GH treatment affect milk production. In a previous report, we showed that the modulation of milk yield due to variations in milking frequency and GH treatment was associated with variations in mammary cell numbers. The aim of this study was to clarify the different me...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2004.04.002
更新日期：2004-11-01 00:00:00
abstract：:We recently discovered that the orphan G-protein-coupled receptor (GPR) 61 colocalized with GnRH receptors (GnRHRs) on the surface of most of bovine gonadotrophs. A recent study suggested that ethanolamine plasmalogen (PI) is a ligand for GPR61 in mouse neuroblastoma. Therefore, this study evaluated the hypothesis tha...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2018.04.002
更新日期：2018-07-01 00:00:00
abstract：:In this study, the expression patterns of inhibins, activins, insulin-like growth factor-I (IGF-I) and steroidogenic enzymes in equine placentae recovered during the latter two-thirds of gestation were examined. Concentrations of inhibin A and inhibin pro-alphaC in endometrial and fetal placental tissue homogenates we...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2005.09.005
更新日期：2006-07-01 00:00:00
abstract：:Considering that recumbency can lead to a change in localization, detectability of distinct margins and thus measurement of the organs and reconstruction quality, it is of value to evaluate the effect of positioning on the objective evaluation of abdominal organs in computed tomography (CT). The objective of the prese...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.106406
更新日期：2020-07-01 00:00:00
abstract：:Feline proinsulin was cloned and expressed using a bacterial expression system. It was then purified from inclusion bodies using size exclusion chromatography and further processed including reduction of the protein. Following refolding, proinsulin was purified by reversed-phase high-performance liquid chromatography ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2005.05.005
更新日期：2006-01-01 00:00:00
