Poloxamer thermogel systems as medium for crystallization.


PURPOSE:To prepare a thermoreversible gel system able to work as a medium for crystallization at around 20°C, allowing easy retrieval of crystals by simply decreasing the gel temperature. Lactose was selected has model substance for crystallization. METHODS:Water solutions with different% of poloxamer 407, α-Lactose monohydrate, and ethanol were prepared and analysed by rheology to understand how the different components alter the gelling temperature. The systems with the required characteristics for lactose crystallization were prepared and the crystals recovered by cooling and then filtering the dispersion. RESULTS:Rheological analysis showed interaction between the poloxamer and lactose. Increasing the quantity of poloxamer or lactose lowered the gelation temperature while the addition of small amounts of ethanol had a modest effect on the same property. These data were used to identify the ideal concentration of the components in order to prepare a system matching the features of our purpose. Such system yielded high quality crystals, with well-defined geometry and narrow particle size distribution. CONCLUSION:Poloxamer is a very interesting polymer in that it is able to generate a reversible gelling medium from which crystals can be harvested by filtering, without the addition of any chemicals to promote the sol-gel transition.


Pharm Res


Pharmaceutical research


Cespi M,Bonacucina G,Casettari L,Mencarelli G,Palmieri GF




Has Abstract


2012-03-01 00:00:00












  • Physicochemical characterization of parenteral lipid emulsion: influence of cosurfactants on flocculation and coalescence.

    abstract:PURPOSE:The stability of lipid emulsions (LE) containing various cosurfactants (oleic acid, cholesterol, Tween 80, or HCO-60) was evaluated using the maximum total interaction energy, Vtmax and the energy barrier for coalescence, W. METHODS:The Vtmax and W were calculated from the zeta potential and the rate of increa...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Yamaguchi T,Nishizaki K,Itai S,Hayashi H,Ohshima H

    更新日期:1995-09-01 00:00:00

  • Nanomechanical properties of selected single pharmaceutical crystals as a predictor of their bulk behaviour.

    abstract:PURPOSE:The main goal of this research was to assess the mechanical properties of APIs' polymorphic forms at the single-crystal level (piroxicam, famotidine, nifedipine, olanzapine) in order to predict their bulk deformational attributes, which are critical for some pharmaceutical technology processes. METHODS:The mec...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Egart M,Janković B,Lah N,Ilić I,Srčič S

    更新日期:2015-02-01 00:00:00

  • Prevention of orthopedic device-associated osteomyelitis using oxacillin-containing biomineral-binding liposomes.

    abstract:PURPOSE:To develop novel biomineral-binding liposomes (BBL) for the prevention of orthopedic implant associated osteomyelitis. METHODS:A biomineral-binding lipid, alendronate-tri(ethyleneglycol)-cholesterol conjugate (ALN-TEG-Chol), was synthesized through Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition (a versatile...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Liu XM,Zhang Y,Chen F,Khutsishvili I,Fehringer EV,Marky LA,Bayles KW,Wang D

    更新日期:2012-11-01 00:00:00

  • Column-switching high-performance liquid chromatography for on-line simultaneous determination and resolution of enantiomers of verapamil and its metabolites.

    abstract::An on-line simultaneous assay for the enantiomers of verapamil (VA) and its three metabolites in plasma was developed with column-switching HPLC. This system consists of an ovomucoid protein chiral stationary phase coupled to an achiral reversed-phase column via a dilution tube and a trapping column. The reversed-phas...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Oda Y,Asakawa N,Kajima T,Yoshida Y,Sato T

    更新日期:1991-08-01 00:00:00

  • Computational Fluid Dynamics (CFD) Simulations of Spray Drying: Linking Drying Parameters with Experimental Aerosolization Performance.

    abstract:PURPOSE:The purpose of this study was to develop a new computational fluid dynamics (CFD)-based model of the complex transport and droplet drying kinetics within a laboratory-scale spray dryer, and relate CFD-predicted drying parameters to powder aerosolization metrics from a reference dry powder inhaler (DPI). METHOD...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Longest PW,Farkas D,Hassan A,Hindle M

    更新日期:2020-05-21 00:00:00

  • Impact of novel MDR modulators on human cancer cells: reversal activities and induction studies.

    abstract:PURPOSE:Novel multidrug resistance (mdr) modulators have been proved as inhibitors of P-glycoprotein (P-gp). We first investigated the in vitro effects of selected compounds in human cancer cells on multidrug resistance reversal effects compared to drug standards and on P-gp induction to characterize the potential of t...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Coburger C,Lage H,Molnár J,Hilgeroth A

    更新日期:2009-01-01 00:00:00

  • Nasal Administration of Cationic Nanoemulsions as Nucleic Acids Delivery Systems Aiming at Mucopolysaccharidosis Type I Gene Therapy.

    abstract:PURPOSE:This study demonstrates the nasal administration (NA) of nanoemulsions complexed with the plasmid encoding for IDUA protein (pIDUA) as an attempt to reach the brain aiming at MPS I gene therapy. METHODS:Formulations composed of DOPE, DOTAP, MCT (NE), and DSPE-PEG (NE-PEG) were prepared by high-pressure homogen...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Schuh RS,Bidone J,Poletto E,Pinheiro CV,Pasqualim G,de Carvalho TG,Farinon M,da Silva Diel D,Xavier RM,Baldo G,Matte U,Teixeira HF

    更新日期:2018-09-26 00:00:00

  • Celiac disease: a challenging disease for pharmaceutical scientists.

    abstract::Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of gluten-containing grains that affects ~1% of the white ethnic population. In the last decades, a rise in prevalence of CD has been observed that cannot be fully explained by improved diagnostics. Genetic predisposition greatly influenc...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Matoori S,Fuhrmann G,Leroux JC

    更新日期:2013-03-01 00:00:00

  • Effects of excipients on protein conformation in lyophilized solids by hydrogen/deuterium exchange mass spectrometry.

    abstract:PURPOSE:Excipients are added to lyophilized protein drug formulations to protect the protein during processing and storage, but the mechanisms are poorly understood. Here, hydrogen/deuterium (H/D) exchange with mass spectrometry was used to assess protein conformation and excipient interactions in lyophilized solids. ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Li Y,Williams TD,Topp EM

    更新日期:2008-02-01 00:00:00

  • Characterizing the expression of CYP3A4 and efflux transporters (P-gp, MRP1, and MRP2) in CYP3A4-transfected Caco-2 cells after induction with sodium butyrate and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate.

    abstract:PURPOSE:To examine the changes in expression levels of CYP3A4 and efflux transporters in CYP3A4-transfected Caco-2 (colon carcinoma) cells in the presence of the inducers sodium butyrate (NaB) and 12-O-tetradecanoylphorbol-13-acetate (TPA). To characterize the transport of [3H]-digoxin and the metabolism of midazolam i...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Cummins CL,Mangravite LM,Benet LZ

    更新日期:2001-08-01 00:00:00

  • Sialic acid 9-O-acetylesterase catalyzes the hydrolyzing reaction from alacepril to deacetylalacepril.

    abstract:PURPOSE:In this work, the alacepril thiolesterase, which catalyzes the hydrolyzing reaction of the thiolester linkage in alacepril and the conversion from alacepril to deacetylalacepril, was purified from rat liver cytosol and characterized. METHODS:A purification procedure for the thiolesterase consisted of ammonium ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Usui S,Kubota M,Iguchi K,Kiho T,Sugiyama T,Katagiri Y,Hirano K

    更新日期:2003-08-01 00:00:00

  • Nanocarriers.

    abstract::The use of nanoparticulate pharmaceutical carriers to enhance the in vivo efficiency of many drugs well established itself over the past decade both in pharmaceutical research and clinical setting. The current level of engineering pharmaceutical nanocarriers in some cases allows for drug delivery systems (DDS) to demo...

    journal_title:Pharmaceutical research

    pub_type: 社论


    authors: Torchilin VP

    更新日期:2007-12-01 00:00:00

  • Vaccine delivery to the oral cavity using coated microneedles induces systemic and mucosal immunity.

    abstract:PURPOSE:The objective of this study is to evaluate the feasibility of using coated microneedles to deliver vaccines into the oral cavity to induce systemic and mucosal immune responses. METHOD:Microneedles were coated with sulforhodamine, ovalbumin and two HIV antigens. Coated microneedles were inserted into the inner...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Ma Y,Tao W,Krebs SJ,Sutton WF,Haigwood NL,Gill HS

    更新日期:2014-09-01 00:00:00

  • Characterization of a nanoparticulate drug delivery system using scanning ion occlusion sensing.

    abstract:PURPOSE:To explore the application of scanning ion occlusion sensing (SIOS) as a novel technology for characterization of nanoparticles. METHODS:Liposomes were employed as model nanoparticles. The size distribution of the liposomes was measured by both SIOS and dynamic light scattering (DLS). Particle number concentra...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Yang L,Broom MF,Tucker IG

    更新日期:2012-09-01 00:00:00

  • In vivo evaluation of enteric-coated naproxen tablets using gamma scintigraphy.

    abstract::Seven healthy, male volunteers were entered into a randomized, open crossover study of the gastrointestinal transit of two enteric-coated 500-mg naproxen tablets. Two radiolabeled tablets were given to each volunteer on two occasions separated by 7 days, once in the fasted state and once after breakfast. Radiolabeling...

    journal_title:Pharmaceutical research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Wilding IR,Hardy JG,Sparrow RA,Davis SS,Daly PB,English JR

    更新日期:1992-11-01 00:00:00

  • Antibacterial Silver-Conjugated Magnetic Nanoparticles: Design, Synthesis and Bactericidal Effect.

    abstract:PURPOSE:The aim was to design and thoroughly characterize monodisperse Fe3O4@SiO2-Ag nanoparticles with strong antibacterial properties, which makes them a candidate for targeting bacterial infections. METHODS:The monodisperse Fe3O4 nanoparticles were prepared by oleic acid-stabilized thermal decomposition of Fe(III) ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Shatan AB,Venclíková K,Zasońska BA,Patsula V,Pop-Georgievski O,Petrovský E,Horák D

    更新日期:2019-08-14 00:00:00

  • A study on structural and diffusion properties of porcine stratum corneum based on very small angle neutron scattering data.

    abstract:PURPOSE:Generation of valuable information about the biphasic geometrical configuration of porcine stratum corneum from Very Small Angle Neutron Scattering (VSANS) data and investigation of its effect on the corresponding effective diffusivity. METHODS:Spectra of porcine stratum corneum are mathematically transformed ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Charalambopoulou GC,Karamertzanis P,Kikkinides ES,Stubos AK,Kanellopoulos NK,Papaioannou AT

    更新日期:2000-09-01 00:00:00

  • Fuzzy modeling of skin permeability coefficients.

    abstract:PURPOSE:The purpose of this work was to determine whether a new modeling methodology using fuzzy logic c an predict skin permeability coefficients that are given compound descriptors that have been proven to affect percutaneous penetration. METHOD:Three fuzzy inference models were developed using subtractive clusterin...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Pannier AK,Brand RM,Jones DD

    更新日期:2003-02-01 00:00:00

  • A smart interface for reliable intradermal injection and infusion of high and low viscosity solutions.

    abstract:PURPOSE:We present a smart intradermal interface suitable for skin-attached drug delivery devices. Our solution enables injections or infusions that are less invasive compared to subcutaneous injections and are leakage-free at the location of penetration. METHODS:The intradermal interface is based on a 31-gauge cannul...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Vosseler M,Jugl M,Zengerle R

    更新日期:2011-03-01 00:00:00

  • Molecular mobility as a predictor of the water sorption by annealed amorphous trehalose.

    abstract:PURPOSE:The work aims at investigating the correlation of water sorption potential with different measures of molecular mobility in an annealed amorphous model compound (trehalose). METHODS:Amorphous trehalose, prepared by freeze-drying, was annealed at 100°C (17°C < T (g)) for up to 120 h. Global mo...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Bhardwaj SP,Suryanarayanan R

    更新日期:2013-03-01 00:00:00

  • Development and in vivo evaluation of an oral delivery system for low molecular weight heparin based on thiolated polycarbophil.

    abstract:PURPOSE:It was the purpose of this study to develop a new oral drug delivery system for low molecular weight heparin (LMWH) providing an improved bioavailability and a prolonged therapeutic effect. METHODS:The permeation enhancing polycarbophil-cysteine conjugate (PCP-Cys) used in this study displayed 111.4 +/- 6.4 mi...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Kast CE,Guggi D,Langoth N,Bernkop-Schnürch A

    更新日期:2003-06-01 00:00:00

  • A Dose Ranging Pharmacokinetic Evaluation of IQP-0528 Released from Intravaginal Rings in Non-Human Primates.

    abstract:PURPOSE:Design of intravaginal rings (IVRs) for delivery of antiretrovirals is often guided by in vitro release under sink conditions, based on the assumption that in vivo release will follow a similar release profile. METHODS:We conducted a dose-ranging study in the female reproductive tract of pigtail macaques using...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Su JT,Teller RS,Srinivasan P,Zhang J,Martin A,Sung S,Smith JM,Kiser PF

    更新日期:2017-10-01 00:00:00

  • Measuring The Bipolar Charge Distributions of Fine Particle Aerosol Clouds of Commercial PMDI Suspensions Using a Bipolar Next Generation Impactor (bp-NGI).

    abstract:PURPOSE:To measure the charge to mass (Q/M) ratios of the impactor stage masses (ISM) from commercial Flixotide™ 250 μg Evohaler, containing fluticasone propionate (FP), Serevent™ 25 μg Evohaler, containing salmeterol xinafoate (SX), and a combination Seretide™ 250/25 μg (FP/SX) Evohaler metered dose inhalers (MDIs). M...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Rowland M,Cavecchi A,Thielmann F,Kulon J,Shur J,Price R

    更新日期:2018-11-26 00:00:00

  • Intra- and intersubject variability: mixed-effects statistical analysis of repeated doses of an angiotensin converting enzyme inhibitor, CGS 16617.

    abstract::The intrasubject and intersubject variabilities for CGS 16617, an angiotensin converting enzyme inhibitor, were evaluated in an open-label, repeat single-dose bioavailability trial. Eight healthy male volunteers each received a 20-mg oral dose of CGS 16617 as an aqueous solution on four separate occasions. Components ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Kochak GM,Smith RA,Choi RL,John V,Tipnis V,Honc F,deSilva JK,Weidler DJ

    更新日期:1989-04-01 00:00:00

  • Identification of human UDP-glucuronosyltransferase responsible for the glucuronidation of niflumic acid in human liver.

    abstract:PURPOSE:To assess the uridine diphosphate (UDP)-glucuronosyltransferase (UGT) isozymes involved in the glucuronidation of niflumic acid in human liver. METHODS:The glucuronidation activity of niflumic acid was determined in liver microsomes and recombinant UGT isozymes by incubation of niflumic acid with UDP-glucuroni...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Mano Y,Usui T,Kamimura H

    更新日期:2006-07-01 00:00:00

  • Pharmacokinetics of antimony in patients treated with sodium stibogluconate for cutaneous leishmaniasis.

    abstract::The pharmacokinetics of Sb was examined in 29 patients with cutaneous leishmaniasis following the intramuscular administration of a dose of sodium stibogluconate equivalent to 600 mg of Sb. Blood was sampled at different time intervals from each patient and Sb was measured in whole blood by electrothermal atomic absor...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Jaser MA,el-Yazigi A,Croft SL

    更新日期:1995-01-01 00:00:00

  • Enhancement of the physical stability of amorphous indomethacin by mixing it with octaacetylmaltose. inter and intra molecular studies.

    abstract:PURPOSE:To demonstrate a very effective and easy way of stabilization of amorphous indomethacin (IMC) by preparing binary mixtures with octaacetylmaltose (acMAL). In order to understand the origin of increased stability of amorphous system inter- and intramolecular interactions between IMC and acMAL were studied. METH...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Kaminska E,Adrjanowicz K,Zakowiecki D,Milanowski B,Tarnacka M,Hawelek L,Dulski M,Pilch J,Smolka W,Kaczmarczyk-Sedlak I,Kaminski K

    更新日期:2014-10-01 00:00:00

  • Determination of acrolein in urine by liquid chromatography and fluorescence detection of its quinoline derivative.

    abstract::We describe an assay for acrolein in urine, employing derivatization with m-aminophenol in the presence of ferrous sulfate solution in sulfuric acid. The derivative (7-OH quinoline; DER) and the internal standard (quinine-bisulfate; IS) were separated on a 10-micron particle, 8 mm x 10-cm C18 cartridge in conjunction ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: al-Rawithi S,el-Yazigi A,Nicholls PJ

    更新日期:1993-11-01 00:00:00

  • A photo-activated targeting chemotherapy using glutathione sensitive camptothecin-loaded polymeric micelles.

    abstract:PURPOSE:A novel photo-activated targeted chemotherapy was developed by photochemical internalization (PCI) of glutathione-sensitive polymeric micelles incorporating camptothecin (CPT) prepared from thiolated CPT (CPT-DP) and thiolated poly(ethylene glycol)-b-poly(glutamic acid) (PEG-b-P(Glu-DP)) METHODS: PEG-b-P(Glu-DP...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Cabral H,Nakanishi M,Kumagai M,Jang WD,Nishiyama N,Kataoka K

    更新日期:2009-01-01 00:00:00

  • Irinotecan Alters the Disposition of Morphine Via Inhibition of Organic Cation Transporter 1 (OCT1) and 2 (OCT2).

    abstract:PURPOSE:The organic cation transporters (OCTs) and multidrug and toxin extrusions (MATEs) together are regarded as an organic cation transport system critical to the disposition and response of many organic cationic drugs. Patient response to the analgesic morphine, a characterized substrate for human OCT1, is highly v...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Zhu P,Ye Z,Guo D,Xiong Z,Huang S,Guo J,Zhang W,Polli JE,Zhou H,Li Q,Shu Y

    更新日期:2018-10-25 00:00:00