Abstract：
:The majority of common bean plants are cultivated under drought conditions. Maintaining crop yields under drought stress is thus one of the biggest challenges facing bean production. In order to improve our understanding of the complex mechanisms involved in the response of common bean (Phaseolus vulgaris) to drought stress, a proteomic approach was used to identify drought-responsive proteins in leaves of two cultivars differing in their response to drought, Tiber and more sensitive Starozagorski čern. 2D-DIGE was used to compare differences in protein abundance between control and stressed plants. Fifty-eight proteins whose abundance changed significantly were identified by LC-MS/MS in Tiber and 64 in Starozagorski čern. The majority of identified proteins were classified into functional categories that include energy metabolism, photosynthesis, ATP interconversion, protein synthesis and proteolysis, stress and defence related proteins. Details of the function of the identified proteins and their abundance profiles in Tiber and Starozagorski are discussed. Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways and molecular functions affected by drought stress. The results form the basis for a further understanding of the biochemical mechanisms of drought response in common bean.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Zadražnik T,Hollung K,Egge-Jacobsen W,Meglič V,Šuštar-Vozlič Jdoi
10.1016/j.jprot.2012.09.021subject
Has Abstractpub_date
2013-01-14 00:00:00pages
254-72eissn
1874-3919issn
1876-7737pii
S1874-3919(12)00665-3journal_volume
78pub_type
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