Abstract:
PURPOSE:We aimed to assess the fate of β-arteether lipid-based drug delivery systems (AE-LBDDS) in terms of resistance to lipolysis and permeation across intestinal cells. METHODS:AE-LBDDS contained Tween 80 or Cremophor EL as surfactants, ethanol, Maisine 35-1 and vegetable oil. The solubilization behavior of AE was investigated during dynamic in vitro lipolysis. The permeation of AE-LBDDS was evaluated using Caco-2, HT29-MTX and M cell monolayers. RESULTS:A higher level of AE precipitation was observed for formulations containing Cremophor EL (~30%) compared to formulations containing Tween 80 (~10%) after lipolysis. However, rapid re-dissolution of the precipitated AE from LBDDS containing Cremophor EL in the intestinal biorelevant media was observed. The transport of AE loaded in LBDDS was enhanced in comparison to that of free drug due to the increased AE solubility. The apparent permeability of all AE-LBDDS across Caco-2 cell monolayers was approximately 3.10(-6) cm/s. A decrease in the permeability was observed at 4°C. M cells did not influence the transport of AE-LBDDS, and mucus decreased AE permeability when formulated with Tween 80. Furthermore, AE is not a P-glycoprotein substrate. CONCLUSION:LBDDS that are partly resistant to in vitro lipolysis significantly increased the transport of AE across intestinal cell monolayers.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Memvanga PB,Eloy P,Gaigneaux EM,Préat Vdoi
10.1007/s11095-013-1094-4subject
Has Abstractpub_date
2013-10-01 00:00:00pages
2694-705issue
10eissn
0724-8741issn
1573-904Xjournal_volume
30pub_type
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