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Intramuscular therapeutic vaccination targeting HPV16 induces T cell responses that localize in mucosal lesions.

Abstract：

:About 25% of high-grade cervical intraepithelial neoplasias (CIN2/3) caused by human papillomavirus serotype 16 (HPV16) undergo complete spontaneous regression. However, to date, therapeutic vaccination strategies for HPV disease have yielded limited success when measured by their ability to induce robust peripheral blood T cell responses to vaccine antigen. We report marked immunologic changes in the target lesion microenvironment after intramuscular therapeutic vaccination targeting HPV16 E6/E7 antigens, in subjects with CIN2/3 who had modest detectable responses in circulating T lymphocytes. Histologic and molecular changes, including markedly (average threefold) increased intensity of CD8(+) T cell infiltrates in both the stromal and epithelial compartments, suggest an effector response to vaccination. Postvaccination cervical tissue immune infiltrates included organized tertiary lymphoid-like structures in the stroma subjacent to residual intraepithelial lesions and, unlike infiltrates in unvaccinated lesions, showed evidence of proliferation induced by recognition of cognate antigen. At a molecular level, these histologic changes in the stroma were characterized by increased expression of genes associated with immune activation (CXCR3) and effector function (Tbet and IFNβ), and were also associated with an immunologic signature in the overlying dysplastic epithelium. High-throughput T cell receptor sequencing of unmanipulated specimens identified clonal expansions in the tissue that were not readily detectable in peripheral blood. Together, these findings indicate that peripheral therapeutic vaccination to HPV antigens can induce a robust tissue-localized effector immune response, and that analyses of immune responses at sites of antigen are likely to be much more informative than analyses of cells that remain in the circulation.

journal_name

Sci Transl Med

journal_title

Science translational medicine

authors

Maldonado L,Teague JE,Morrow MP,Jotova I,Wu TC,Wang C,Desmarais C,Boyer JD,Tycko B,Robins HS,Clark RA,Trimble CL

doi

10.1126/scitranslmed.3007323

subject

Has Abstract

pub_date

2014-01-29 00:00:00

pages

221ra13

issue

221

eissn

1946-6234

issn

1946-6242

journal_volume

pub_type

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Intramuscular therapeutic vaccination targeting HPV16 induces T cell responses that localize in mucosal lesions.