Abstract：
:Most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. The aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. The list of targets include, among the others, Listeria spp., Salmonella spp., Shigella spp., Escherichia coli spp., Campylobacter spp., Clostridium spp. and Staphylococcus aureus. The accuracy of detection was determined by using ATCC strains as positive and negative controls. The achieved sensitivity of each of assays was 1 pg of genomic DNA, which was equivalent to ∼1 cfu. The working ranges of the TaqMan(®) Real-time PCR assays, when used quantitatively on cheese and meat samples inoculated with serial dilution of Listeria spp., Listeria monocytogenes, S. aureus, Salmonella enterica, Shigella boydii, E. coli O157:H7, Bacillus cereus, Campylobacter coli, Yersinia enterocolitica, Enterobacter sakazakii and Pseudomonas aeruginosa was 10(8) cfu/g to 10(4) cfu/g. No matrix interferences were observed.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
Cremonesi P,Pisani LF,Lecchi C,Ceciliani F,Martino P,Bonastre AS,Karus A,Balzaretti C,Castiglioni Bdoi
10.1016/j.fm.2014.04.007subject
Has Abstractpub_date
2014-10-01 00:00:00pages
35-40eissn
0740-0020issn
1095-9998pii
S0740-0020(14)00082-3journal_volume
43pub_type
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