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Development of 23 individual TaqMan® real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions.

Abstract：

:Most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. The aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. The list of targets include, among the others, Listeria spp., Salmonella spp., Shigella spp., Escherichia coli spp., Campylobacter spp., Clostridium spp. and Staphylococcus aureus. The accuracy of detection was determined by using ATCC strains as positive and negative controls. The achieved sensitivity of each of assays was 1 pg of genomic DNA, which was equivalent to ∼1 cfu. The working ranges of the TaqMan(®) Real-time PCR assays, when used quantitatively on cheese and meat samples inoculated with serial dilution of Listeria spp., Listeria monocytogenes, S. aureus, Salmonella enterica, Shigella boydii, E. coli O157:H7, Bacillus cereus, Campylobacter coli, Yersinia enterocolitica, Enterobacter sakazakii and Pseudomonas aeruginosa was 10(8) cfu/g to 10(4) cfu/g. No matrix interferences were observed.

journal_name

Food Microbiol

journal_title

Food microbiology

authors

Cremonesi P,Pisani LF,Lecchi C,Ceciliani F,Martino P,Bonastre AS,Karus A,Balzaretti C,Castiglioni B

doi

10.1016/j.fm.2014.04.007

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

35-40

eissn

0740-0020

issn

1095-9998

pii

S0740-0020(14)00082-3

journal_volume

43

pub_type

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