当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY期刊下所有文献 > Genetic and serological identification of three Vibrio parahaemolyticus strains as candidates for novel provisional O serotypes.

Genetic and serological identification of three Vibrio parahaemolyticus strains as candidates for novel provisional O serotypes.

Abstract：

:Vibrio parahaemolyticus is a Gram-negative, halophilic Vibrio that naturally inhabits marine and estuarine environments worldwide and has recently been recognized as one of the most important foodborne pathogens. To date, 13 O serotypes and 71 K serotypes of V. parahaemolyticus have been identified. However, untypeable V. parahaemolyticus strains are frequently found during routine detection, indicating that other forms of serotypes exist and suggesting the necessity for extension of the antigenic scheme. In this work, through the genetic analysis of the O serotype genetic determinants (OGDs) and the production of antisera and serological tests, we identified three novel O serotypes of V. parahaemolyticus. Further analyses showed that recombination and gene-set deletions/insertions within OGDs may play key roles in the generation of V. parahaemolyticus O serotype diversity. A PCR method was developed for the identification of these novel O serotypes, and specificity and sensitivity were evaluated. A double-blind test including 283 clinical isolates was performed, giving perfect correlation with the agglutination test results. Generally, our study expanded the O-antigenic scheme of V. parahaemolyticus from 13 to 16 and provided a tool with the potential for the detection and identification of V. parahaemolyticus strains (especially untypeable strains) isolated from both the clinic and the environment.

journal_name

Int J Food Microbiol

journal_title

International journal of food microbiology

authors

Guo X,Liu B,Chen M,Wang Y,Wang L,Chen H,Wang Y,Tu L,Zhang X,Feng L

doi

10.1016/j.ijfoodmicro.2017.01.010

subject

Has Abstract

pub_date

2017-03-20 00:00:00

pages

53-58

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(17)30034-X

journal_volume

245

pub_type

杂志文章

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