Responses of Listeria monocytogenes to acid stress and glucose availability monitored by measurements of intracellular pH and viable counts.

Abstract:

:Physiological aspects of the response of Listeria monocytogenes to acidic conditions and effect of glucose availability were studied by fluorescence ratio-imaging microscopy (FRIM) as compared with traditional viable counts. Three types of experiments were conducted: (i) static with measurements of intracellular pH (pHi) at extracellular pH (pHo) values ranging from pH 3.0 to 6.0 at 0.5 pH unit intervals; (ii) kinetic with monitoring of bacterial responses to changes in the pHo from the value of 6.0 to 4.0 or 3.0; (iii) survival experiments studying bacterial recovery in response to a shift to favourable conditions after a treatment at low pH. All the experiments were performed at three levels of glucose in the medium (0, 1, and 10 mM). Both survival and pHi were greatly affected by pHo and glucose availability with the highest values for CFU and pHi at highest glucose concentration and pHo values in the medium in all trials. A high correlation (R2 = 0.995) between pHi and CFU counts was observed. The pH gradient started to collapse at pHo 4 and below for trials with glucose in the medium and at pHo 5.5 and below without glucose. A recovery step was proposed after the apparently lethal treatment to assess cell viability by FRIM.

journal_name

Int J Food Microbiol

authors

Shabala L,Budde B,Ross T,Siegumfeldt H,McMeekin T

doi

10.1016/s0168-1605(01)00740-1

subject

Has Abstract

pub_date

2002-05-05 00:00:00

pages

89-97

issue

1-2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(01)00740-1

journal_volume

75

pub_type

杂志文章
  • Migration of Caenorhabditis elegans to manure and manure compost and potential for transport of Salmonella newport to fruits and vegetables.

    abstract::A study was done to determine if a free-living, bacterivorous nematode, Caenorhabditis elegans, migrates to bovine manure, turkey manure, composted bovine manure, composted turkey manure, and manure-amended soil inoculated with Salmonella Newport. Movement of the worm to lettuce, strawberries, and carrots was also stu...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2005.05.011

    authors: Kenney SJ,Anderson GL,Williams PL,Millner PD,Beuchat LR

    更新日期:2006-01-15 00:00:00

  • Molecular response of Saccharomyces cerevisiae wine and laboratory strains to high sugar stress conditions.

    abstract::One of the stress conditions that can affect Saccharomyces cerevisiae cells during their growth is osmotic stress. Under particular environments (for instance, during the production of alcoholic beverages) yeasts have to cope with osmotic stress caused by high sugar concentrations. Although the molecular changes and p...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2010.12.023

    authors: Jiménez-Martí E,Zuzuarregui A,Gomar-Alba M,Gutiérrez D,Gil C,del Olmo M

    更新日期:2011-01-31 00:00:00

  • A chemically defined minimal medium for the optimal culture of Listeria.

    abstract::Unlike most other bacteria, many Listeria strains do not grow well in the minimal media described so far in the literature. Among the minimal media tested, a chemically defined medium modified from that of Premaratne and co-workers was found to support the best growth of Listeria spp. The promoting effect was due to t...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(96)01205-6

    authors: Phan-Thanh L,Gormon T

    更新日期:1997-03-18 00:00:00

  • Detection of oxazolidinone and phenicol resistant enterococcal isolates from duck feces and carcasses.

    abstract::The heavy use or abuse of antimicrobials in food animals has caused an increase in antimicrobial resistance in enterococci of animal origin, which could get transmitted to those of human origin via the food chain. Since duck meat consumption has been on the rise in Korea, we conducted this study to provide information...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2019.01.002

    authors: Na SH,Moon DC,Choi MJ,Oh SJ,Jung DY,Kang HY,Hyun BH,Lim SK

    更新日期:2019-03-16 00:00:00

  • Changes in the microflora on commercial beef trimmings during their collection, distribution and preparation for retail sale as ground beef.

    abstract::A commercial process for ground meat production, from the collection of meat plant trimmings to the retailing of consumer packs of ground beef, was examined. The selected process compiled with current concepts of Good Manufacturing Practice. During storage for up to 18 days before grinding, most trimming developed a f...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/0168-1605(93)90154-9

    authors: Gill CO,McGinnis C

    更新日期:1993-06-01 00:00:00

  • Enterobacter sakazakii bacteriophages can prevent bacterial growth in reconstituted infant formula.

    abstract::Reconstituted infant formula has been implicated in outbreaks of Enterobacter sakazakii infections, causing high mortality and serious sequelae. Current prevention methods appear to be insufficient to ensure that such foods are free of E. sakazakii. In this study, the usefulness of bacteriophages for biocontrol of E. ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2006.10.029

    authors: Kim KP,Klumpp J,Loessner MJ

    更新日期:2007-04-10 00:00:00

  • Applicability of the EN ISO 11290-1 standard method for Listeria monocytogenes detection in presence of new Listeria species.

    abstract::During the past six years, new species of the genus Listeria have been isolated from foods and other environmental niches worldwide. The Standard method EN ISO 11290-1 that is currently under revision will include in its scope all Listeria species in addition to L. monocytogenes. The objective of this project was to e...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2016.09.028

    authors: Barre L,Angelidis AS,Boussaid D,Brasseur ED,Manso E,Gnanou Besse N

    更新日期:2016-12-05 00:00:00

  • Growth of Shigella flexneri in foods: comparison of observed and predicted growth kinetics parameters.

    abstract::Shigella causes foodborne gastrointestinal illness; however, little information is available on its ability to grow in foods. Commercially available sterile foods (UHT milk, beef broth, chicken broth, vegetable broth, meats, vegetables) were inoculated with S. flexneri 5348 and incubated at 12, 15, 19, 28 or 37 degree...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/0168-1605(96)01109-9

    authors: Zaika LL,Scullen OJ

    更新日期:1996-09-01 00:00:00

  • Contamination profiles and characterisation of Bacillus species in wheat bread and raw materials for bread production.

    abstract::The Bacillus counts in white and wholemeal wheat loaves produced without preservatives or sour dough were consistently 10(6) cfu/g after two days of storage at ambient summer temperatures (25-30 degree C). Identified species were B. subtilis (70%), B. licheniformis (24%), B. pumilus (2%) and B. cereus (2%). The domina...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/0168-1605(94)00147-x

    authors: Rosenkvist H,Hansen A

    更新日期:1995-08-01 00:00:00

  • Biocontrol of Aspergillus flavus on peanut kernels by use of a strain of marine Bacillus megaterium.

    abstract::A strain of marine Bacillus megaterium isolated from the Yellow Sea of East China was evaluated for its activity in reducing postharvest decay of peanut kernels caused by Aspergillus flavus in in vitro and in vivo tests. The results showed that the concentrations of antagonist had a significant effect on biocontrol ef...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2010.01.036

    authors: Kong Q,Shan S,Liu Q,Wang X,Yu F

    更新日期:2010-04-30 00:00:00

  • Immunological methods for detection of foodborne pathogens and their toxins.

    abstract::Improved methods to detect microorganisms and their toxins introduced during the last decade involve among others recombinant DNA techniques and various immuno-assays such as the enzyme-linked immunosorbent assay and the latex agglutination. Immuno-assays are based on a quantitative reaction of an antigen (bacterial m...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/0168-1605(91)90050-y

    authors: Notermans S,Wernars K

    更新日期:1991-01-01 00:00:00

  • Modelling the effect of temperature and water activity on the growth rate of Aspergillus flavus and aflatoxin production in peanut meal extract agar.

    abstract::Aspergillus flavus is the predominant species that produce aflatoxins in stored peanuts under favourable conditions. This study aimed to describe the growth and aflatoxin production by two A. flavus strains isolated from imported raw peanuts and to model the effects of temperature and aw on their colony growth rate as...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2020.108836

    authors: Norlia M,Jinap S,Nor-Khaizura MAR,Radu S,John JM,Rahman MAH,Peter ML,Sharif Z

    更新日期:2020-12-16 00:00:00

  • Quorum sensing signals affect spoilage of refrigerated large yellow croaker (Pseudosciaena crocea) by Shewanella baltica.

    abstract::In this work we investigated the specific spoilage organism (SSO) of large yellow croaker (Pseudosciaena crocea) stored at 4°C and role of quorum sensing (QS) system of SSO isolated from the spoiled fish. According to microbial count and 16S rRNA gene of the isolated pure strains, Shewanella, mainly Shewanella baltica...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2015.10.020

    authors: Zhu J,Zhao A,Feng L,Gao H

    更新日期:2016-01-18 00:00:00

  • Screening of various foodstuffs for occurrence of Coxiella burnetii in Switzerland.

    abstract::The epidemiology of Q-fever in Switzerland is largely unknown. For this reason, a screening programme for the presence of Coxiella burnetii in bulk milk samples from cows, sheep and goats and in shell eggs produced in and imported into Switzerland was conducted. In total, 17 of 359 (4.7%) of analysed bovine milk sampl...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2007.03.001

    authors: Fretz R,Schaeren W,Tanner M,Baumgartner A

    更新日期:2007-05-30 00:00:00

  • Water UV-C treatment alone or in combination with peracetic acid: A technology to maintain safety and quality of strawberries.

    abstract::Disinfection of fruits is one of the most important steps since they are going to be eaten fresh-or minimally-processed. This step affects quality, safety, and shelf-life of the product. Despite being a common sanitizer in the fruit industry, chlorine may react with organic matter leading to the formation of toxic by-...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2020.108887

    authors: Nicolau-Lapeña I,Abadias M,Viñas I,Bobo G,Lafarga T,Ribas-Agustí A,Aguiló-Aguayo I

    更新日期:2020-12-16 00:00:00

  • Inhibition of bacterial foodborne pathogens by the lactoperoxidase system in combination with monolaurin.

    abstract::The lactoperoxidase system (LPS) and monolaurin (ML) are potential natural antimicrobial agents for use in foods. The LPS is considered to have greatest activity against Gram-negative bacteria while ML is usually considered to have greatest activity against Gram-positive bacteria. An LPS-ML combination system (utilizi...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(01)00698-5

    authors: McLay JC,Kennedy MJ,Orourke AL,Elliot RM,Simmonds RS

    更新日期:2002-02-25 00:00:00

  • Characterisation of commercial and natural Torulaspora delbrueckii wine yeast strains.

    abstract::Forty-three South African Torulaspora delbrueckii yeast isolates from the ARC Infruitec-Nietvoorbij yeast culture collection, the T. delbrueckii type strain (CBS 1146), one reference T. delbrueckii strain (CBS 4663), two T. delbrueckii strains isolated from commercial yeast blends (Viniflora® Harmony.nsac and Viniflor...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2013.02.011

    authors: van Breda V,Jolly N,van Wyk J

    更新日期:2013-05-15 00:00:00

  • Development of a real-time PCR method coupled with a selective pre-enrichment step for quantification of Morganella morganii and Morganella psychrotolerans in fish products.

    abstract::Histamine fish poisoning is common and due to toxic concentrations of histamine often produced by Gram-negative bacteria in fin-fish products with a high content of the free amino acid histidine. The genus Morganella includes two species previously reported to cause incidents of histamine fish poisoning. Morganella mo...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2015.03.005

    authors: Podeur G,Dalgaard P,Leroi F,Prévost H,Emborg J,Martinussen J,Hansen LH,Pilet MF

    更新日期:2015-06-16 00:00:00

  • The impact of water and temperature interactions on lag phase, growth and potential ochratoxin A production by two new species, Aspergillus aculeatinus and A. sclerotiicarbonarius, on a green coffee-based medium.

    abstract::Two new species of Aspergillus (A. aculeatinus, A. sclerotiicarbonarius) were previously isolated from coffee in Thailand. The objective of this study was to examine the effect of interacting environmental factors of water availability (water activity, aw) and temperature on lag phases prior to growth, growth and pote...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2014.07.025

    authors: Akbar A,Magan N

    更新日期:2014-10-01 00:00:00

  • Occurrence, antimicrobial resistance and whole-genome sequencing analysis of Salmonella isolates from chicken carcasses imported into Iraq from four different countries.

    abstract::Salmonella is a major cause of human foodborne illnesses worldwide; however, little is known about its occurrence and genomic characteristics in food sources in many developing countries. This study investigates the occurrence, serotypes distribution, antimicrobial resistance, and multilocus sequence types (ST) of Sal...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2018.07.007

    authors: Harb A,Habib I,Mezal EH,Kareem HS,Laird T,O'Dea M,Abraham S

    更新日期:2018-11-02 00:00:00

  • Phage-resistance linked to cell heterogeneity in the commercial strain Lactobacillus delbrueckii subsp. lactis Ab1.

    abstract::The aim of this work was to study the relationship between the cell morphological heterogeneity and the phage-resistance in the commercial strain Lactobacillus delbrueckii subsp. lactis Ab1. Two morphological variants (named C and T) were isolated from this strain. Phage-resistant derivatives were isolated from them a...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2008.09.007

    authors: Suárez VB,Maciel N,Guglielmotti D,Zago M,Giraffa G,Reinheimer J

    更新日期:2008-12-10 00:00:00

  • Identification of major contamination sources during processing of emulsion sausage.

    abstract::The extent of contamination of an emulsion type of sausage with lactic acid bacteria was determined along the processing line. This was done by aseptically removing sausages after five different processing stages (heat processing, chilling, cold storage, cutting down and packing). Removed sausages were vacuum-packed a...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/0168-1605(88)90058-x

    authors: Borch E,Nerbrink E,Svensson P

    更新日期:1988-12-31 00:00:00

  • Development of two specific multiplex qPCRs to determine amounts of Pseudomonas, Enterobacteriaceae, Brochothrix thermosphacta and Staphylococcus in meat and heat-treated meat products.

    abstract::Culturing methods are conventionally applied to investigate the contamination of food with several microorganisms after heat processing. However, with these methods, it is not possible to evaluate whether heat-treated meat products, such as cooked sausages, contained parts of spoiled meat. Therefore, two specific mult...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2020.108932

    authors: Bahlinger E,Dorn-In S,Beindorf PM,Mang S,Kaltner F,Gottschalk C,Gareis M,Schwaiger K

    更新日期:2021-01-16 00:00:00

  • Characteristics and significance of yeasts' involvement in cassava fermentation for 'fufu' production.

    abstract::Six different strains of yeast, namely Candida krusei, C. tropicalis, Pichia saitoi, Saccharomyces cerevisiae, P. anomala and Zygosaccharomyces bailii were found present in cassava-fermenting water in the early part of the fermentation. The latter part of the fermentation was dominated in all cases by three strains of...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(01)00431-7

    authors: Oyewole OB

    更新日期:2001-05-10 00:00:00

  • Characterization of four Paenibacillus species isolated from pasteurized, chilled ready-to-eat meals.

    abstract::Food spoilage is often caused by microorganisms. The predominant spoilage microorganisms of pasteurized, chilled ready-to-eat (RTE) mixed rice-vegetable meals stored at 7°C were isolated and determined as Paenibacillus species. These sporeforming psychrotrophic bacteria are well adapted to grow in the starch-rich envi...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2017.04.008

    authors: Helmond M,Nierop Groot MN,van Bokhorst-van de Veen H

    更新日期:2017-07-03 00:00:00

  • Microbiology of processed edible insect products - Results of a preliminary survey.

    abstract::Little is known of the microbiology of processed insect products. The present survey analysed a total of n=38 samples of deep-fried and spiced (Acheta domesticus, Locusta migratoria, and Omphisa fuscidentalis), cooked in soy sauce ("tsukudani"; Oxya yezoensis, Vespula flaviceps, and Bombyx mori), dried (A. domesticus,...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2016.11.005

    authors: Grabowski NT,Klein G

    更新日期:2017-02-21 00:00:00

  • Analysis of yeast and archaeal population dynamics in kimchi using denaturing gradient gel electrophoresis.

    abstract::Kimchi is a traditional Korean food that is fermented from vegetables such as Chinese cabbage and radish. Many bacteria are involved in kimchi fermentation and lactic acid bacteria are known to perform significant roles. Although kimchi fermentation presents a range of environmental conditions that could support many ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2008.05.013

    authors: Chang HW,Kim KH,Nam YD,Roh SW,Kim MS,Jeon CO,Oh HM,Bae JW

    更新日期:2008-08-15 00:00:00

  • Prevalence of Clostridium botulinum types B, E and F in faecal samples from Swedish cattle.

    abstract::Faeces were collected from 60 cows at three slaughterhouses situated in southern and central Sweden. The faecal samples were collected during two sampling periods over the year, summer and winter. All samples were analysed for the presence of Clostridium botulinum spores, according to a combined selection and enrichme...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(02)00255-6

    authors: Dahlenborg M,Borch E,Rådström P

    更新日期:2003-04-25 00:00:00

  • Sporicidal action of ozone and hydrogen peroxide: a comparative study.

    abstract::Elimination of contaminating spores on packaging materials and food-contact surfaces remains a challenge to the food industry. Hydrogen peroxide and chlorine are the most commonly used sanitizers to eliminate these contaminants, and ozone was recommended recently as an alternative. Hence, we compared the sporicidal ac...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(01)00561-x

    authors: Khadre,Yousef AE

    更新日期:2001-12-30 00:00:00

  • Taxonomy and physiology of probiotic lactic acid bacteria.

    abstract::The current taxonomy of probiotic lactic acid bacteria is reviewed with special focus on the genera Lactobacillus, Bifidobacterium and Enterococcus. The physiology and taxonomic position of species and strains of these genera were investigated by phenotypic and genomic methods. In total, 176 strains, including the typ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(98)00049-x

    authors: Klein G,Pack A,Bonaparte C,Reuter G

    更新日期:1998-05-26 00:00:00