Abstract：
:The aim of this study was to compare the sequence of the astA gene found in 8 Korean and 11 Japanese Escherichia coli isolates. Conventional PCR was used to amplify the astA gene from the chromosomal and plasmid DNA preparation samples of each isolate using commercial DNA extraction kits. Cloning of the PCR products, sequence analysis, and pulse field gel electrophoresis (PFGE) were sequentially performed. An identical copy of astA in each isolate were found for 8 Korean and 8 Japanese E. coli strains isolated from bovine, porcine, and healthy human carriers. Among these, 1 Korean and 4 Japanese isolates carried a stop mutation at residue 16. Three Japanese outbreak strains (V199, V638, and 96-127-23) carried multiple clones of astA gene with multiple amino acids changes at residues 11, 16, 20, 23, 30, 33, and 34. Compared with the non-diarrheal isolates, clonal diversity and sequence variations of the astA gene in outbreak isolates may be associated with virulence potential of EAST1.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Seo DJ,Choi S,Jeon SB,Jeong S,Park H,Lee BH,Kim GB,Yang SJ,Nishikawa Y,Choi Cdoi
10.1016/j.ijfoodmicro.2016.11.017subject
Has Abstractpub_date
2017-02-21 00:00:00pages
1-8eissn
0168-1605issn
1879-3460pii
S0168-1605(16)30619-5journal_volume
243pub_type
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journal_title：International journal of food microbiology
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