Abstract:
:Fossil fuels, e.g. gasoline and diesel oil, account for substantial share of the pollution that affects marine ecosystems. Environmental metabolomics is an emerging field that may help unravel the effect of these xenobiotics on seaweeds and provide methodologies for biomonitoring coastal ecosystems. In the present study, FTIR and multivariate analysis were used to discriminate metabolic profiles of Ulva lactuca after in vitro exposure to diesel oil and gasoline, in combinations of concentrations (0.001%, 0.01%, 0.1%, and 1.0% - v/v) and times of exposure (30min, 1h, 12h, and 24h). PCA and HCA performed on entire mid-infrared spectral window were able to discriminate diesel oil-exposed thalli from the gasoline-exposed ones. HCA performed on spectral window related to the protein absorbance (1700-1500cm-1) enabled the best discrimination between gasoline-exposed samples regarding the time of exposure, and between diesel oil-exposed samples according to the concentration. The results indicate that the combination of FTIR with multivariate analysis is a simple and efficient methodology for metabolic profiling with potential use for biomonitoring strategies.
journal_name
Mar Pollut Bulljournal_title
Marine pollution bulletinauthors
Pilatti FK,Ramlov F,Schmidt EC,Costa C,Oliveira ER,Bauer CM,Rocha M,Bouzon ZL,Maraschin Mdoi
10.1016/j.marpolbul.2016.11.006subject
Has Abstractpub_date
2017-01-30 00:00:00pages
831-836issue
2eissn
0025-326Xissn
1879-3363pii
S0025-326X(16)30902-Xjournal_volume
114pub_type
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