Abstract:
:Flow cytometry provides a tool to physically sort single algal cells in order to obtain clonal cultures. During sorting, cells are submitted to physical stress factors such as high fluidic pressure, exposure to the laser beam, electrostatic charges, deflection through high voltage fields, and collisions with container surfaces. All of these can damage the cells of interest and success rates for initiation of cultures from flow-sorted cells are generally very low. We found that the addition of bovine serum albumin in the culture medium into which cells were sorted drastically improved the success of initiation of pico- and nano-eukaryotic phytoplankton strains. Adding a mixture of antibiotics (Penicillin, Neomycin, Streptomycin) to the medium in order to slow down bacterial growth further improved culture development. This approach was successfully used to isolate taxonomically diverse strains, including novel taxa, from a fresh sample obtained in the English Channel and from enrichment cultures established during an Atlantic meridional transect cruise. We anticipate that these improvements will be useful to clone or purify existing cultures and to isolate novel cultures from oceanic samples.
journal_name
J Phycoljournal_title
Journal of phycologyauthors
Marie D,Le Gall F,Edern R,Gourvil P,Vaulot Ddoi
10.1111/jpy.12495subject
Has Abstractpub_date
2017-04-01 00:00:00pages
271-282issue
2eissn
0022-3646issn
1529-8817journal_volume
53pub_type
杂志文章abstract::Despite worldwide proliferation of the genus Caulerpa and subsequent effects on benthic communities, little is known about the nutritional physiology of the Caulerpales. Here, we investigated the uptake rates of ammonium, nitrate, amino acids, and phosphate through the fronds and rhizoids + stolon, the internal transl...
journal_title:Journal of phycology
pub_type: 杂志文章
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更新日期:2020-06-01 00:00:00
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journal_title:Journal of phycology
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journal_title:Journal of phycology
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journal_title:Journal of phycology
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12830
更新日期:2019-04-01 00:00:00
abstract::The study of the microalgal growth kinetics is an indispensable tool in all fields of phycology. Knowing the optimal nutrient concentration is an important issue that will help to develop efficient growth systems for these microorganisms. Although nitrogen and phosphorus are well studied for this purpose, sulfur seems...
journal_title:Journal of phycology
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
abstract::Telomeres generally consist of short repeats of minisatellite DNA sequences and are useful in chromosome identification and karyotype analysis. To date, telomeres have not been characterized in the economically important brown seaweed Saccharina japonica, thus its full cytogenetic research and genetic breeding potenti...
journal_title:Journal of phycology
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doi:10.1111/jpy.12497
更新日期:2017-02-01 00:00:00
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journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12302
更新日期:2015-06-01 00:00:00
abstract::Although hypotheses have been proposed and developed to interpret the origins and functions of introns, substantial controversies remain about the mechanism of intron evolution. The availability of introns in the intermediate state is quite helpful for resolving this debate. In this study, a new strain of diatom (deno...
journal_title:Journal of phycology
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12005
更新日期:2012-12-01 00:00:00
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journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.1966.tb04610.x
更新日期:1966-12-01 00:00:00
abstract::Pacific coast species of the red algae Halymenia and Cryptonemia are described Of the 8 previously reported species of Halymenia 3 (H. abyssicola Dawson, H. megaspore Dawson, H refugiensis Dawson) hive been placed in synonymy with other species. Halymenia hollenbergii from southern California is described as new; H co...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.1967.tb04648.x
更新日期:1967-09-01 00:00:00
abstract::Brevetoxin (PbTx) is a neurotoxic secondary metabolite of the dinoflagellate Karenia brevis. We used a novel, fluorescent BODIPY-labeled conjugate of brevetoxin congener PbTx-2 (B-PbTx) to track absorption of the metabolite into a variety of marine microbes. The labeled toxin was taken up and brightly fluoresced in li...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12787
更新日期:2019-02-01 00:00:00
abstract::Light has been identified as one of the main factors affecting seaweed ecophysiology. We investigated the dependence of nutrient metabolism on sun and shade light conditions and whether episodes of upwelling of nutrient-rich subsuperficial water could reduce the summer nutrient limitation driving physiological changes...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2007.00440.x
更新日期:2008-02-01 00:00:00
abstract::Maintaining buoyancy with gas-filled floats (pneumatocysts) is essential for some subtidal kelps to achieve an upright stature and compete for light . However, as these kelps grow up through the water column, pneumatocysts are exposed to substantial changes in hydrostatic pressure, which could cause complications as i...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12776
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journal_title:Journal of phycology
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更新日期:2011-02-01 00:00:00
abstract::Members of the morphologically unusual cyanobacterial family Gomontiellaceae were studied using a polyphasic approach. Cultured strains of Hormoscilla pringsheimii, Starria zimbabweënsis, Crinalium magnum, and Crinalium epipsammum were thoroughly examined, and the type specimen of the family, Gomontiella subtubulosa, ...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12354
更新日期:2015-12-01 00:00:00
abstract::Dissolved organic carbon (DOC) is an important source of carbon and energy for microbes, but whether it can be used by phytoplankton has not been systematically studied, and the underlying molecular metabolism remains poorly understood. Here, we investigated the ability of phytoplankton to utilize glycerol as a repres...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.13031
更新日期:2020-10-01 00:00:00
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journal_title:Journal of phycology
pub_type: 杂志文章
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journal_title:Journal of phycology
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journal_title:Journal of phycology
pub_type: 杂志文章
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更新日期:2017-04-01 00:00:00
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journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.1968.tb04696.x
更新日期:1968-12-01 00:00:00
abstract::Alexandrium ostenfeldii (Paulsen) Balech and Tangen and A. peruvianum (Balech and B.R. Mendiola) Balech and Tangen are morphologically closely related dinoflagellates known to produce potent neurotoxins. Together with Gonyaulax dimorpha Biecheler, they constitute the A. ostenfeldii species complex. Due to the subtle d...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12134
更新日期:2014-02-01 00:00:00
abstract::Large-scale seaweed cultivation has been instrumental in globalizing the seaweed industry since the 1950s. The domestication of seaweed cultivars (begun in the 1940s) ended the reliance on natural cycles of raw material availability for some species, with efforts driven by consumer demands that far exceeded the availa...
journal_title:Journal of phycology
pub_type: 杂志文章,评审
doi:10.1111/jpy.12326
更新日期:2015-10-01 00:00:00
abstract::Inorganic nitrogen has been identified as the major growth-limiting nutritional factor affecting Gracilaria gracilis populations in South Africa. Although the physiological mechanisms implemented by G. gracilis for adaption to low nitrogen environments have been investigated, little is known about the molecular mechan...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12400
更新日期:2016-06-01 00:00:00
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journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1046/j.1529-8817.2000.99106.x
更新日期:2000-08-26 00:00:00
abstract::An adverse consequence of applying morphology-based taxonomic systems to catalog cyanobacteria, which generally are limited in the number of available morphological characters, is a fundamental underestimation of natural biodiversity. In this study, we further dissect the polyphyletic cyanobacterial genus Lyngbya and ...
journal_title:Journal of phycology
pub_type: 杂志文章
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更新日期:2013-12-01 00:00:00
abstract::We studied group I introns in sterile cultures of selected groups of lichen photobionts, focusing on Trebouxia species associated with Xanthoria s. lat. (including Xanthomendoza spp.; lichen-forming ascomycetes). Group I introns were found inserted after position 798 (Escherichia coli numbering) in the large subunit (...
journal_title:Journal of phycology
pub_type: 杂志文章
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更新日期:2013-12-01 00:00:00
abstract::The Aleutian Archipelago coastal ecosystem has undergone a dramatic change in community composition during the past two decades. Following the removal of ∼99% of the sea otters, Enhydra lutris, from the ecosystem, changes to the benthic communities resulted in widespread losses to most of the region's kelp beds and co...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2012.01139.x
更新日期:2012-08-01 00:00:00
abstract::The enormous species diversity of diatoms correlates with the remarkable range of cell sizes in this group. Nuclear DNA content relates fundamentally to cell volume in other eukaryotic cells. The relationship of cell volume to G1 DNA content was determined among selected members of the genus Thalassiosira, one of the ...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2008.00476.x
更新日期:2008-04-01 00:00:00