Adipose-Derived Stem Cells and Vascularized Lymph Node Transfers Successfully Treat Mouse Hindlimb Secondary Lymphedema by Early Reconnection of the Lymphatic System and Lymphangiogenesis.

Abstract:

BACKGROUND:Secondary lymphedema is often observed in postmalignancy treatment of the breast and the gynecologic organs, but effective therapies have not been established in chronic cases even with advanced physiologic operations. Currently, reconstructive surgery with novel approaches has been attempted. METHODS:The hindlimbs of 10-week-old male C57BL/6J mice, after 30-Gy x-irradiation, surgical lymph node dissection, and 5-mm gap creation, were divided into four groups, with vascularized lymph node transfer abdominal flap and 1.0 × 10 adipose-derived stem cells. Lymphatic flow assessment, a water-displacement plethysmometer paw volumetry test, tissue quantification of lymphatic vessels, and functional analysis of lymphatic vessels and nodes were performed. RESULTS:Photodynamic Eye images, using indocyanine green fluorescence, demonstrated immediate staining in subiliac lymph nodes, and linear pattern imaging of the proximal region was observed with the combined treatment of adipose-derived stem cells and vascularized lymph node transfer. Both percentage improvement and percentage deterioration with the combined treatment of adipose-derived stem cells and vascularized lymph node transfer were significantly better than with other treatments (p < 0.05). The numbers of lymphatic vessels with LYVE-1 immunoreactivity significantly increased in mice treated with adipose-derived stem cells (p < 0.05), and B16 melanoma cells were metastasized in groups treated with vascularized lymph node transfers by day 28. CONCLUSIONS:Adipose-derived stem cells increase the number of lymphatic vessels and vascularized lymph node transfers induce the lymphatic flow drainage to the circulatory system. Combined adipose-derived stem cell and vascularized lymph node transfer treatment in secondary lymphedema may effectively decrease edema volume and restore lymphatic function by lymphangiogenesis and the lymphatic-to-venous circulation route.

journal_name

Plast Reconstr Surg

authors

Hayashida K,Yoshida S,Yoshimoto H,Fujioka M,Saijo H,Migita K,Kumaya M,Akita S

doi

10.1097/PRS.0000000000003110

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

639-651

issue

3

eissn

0032-1052

issn

1529-4242

pii

00006534-201703000-00030

journal_volume

139

pub_type

杂志文章
  • Immature versus mature dura mater: II. Differential expression of genes important to calvarial reossification.

    abstract::The ability of immature animals and newborns to orchestrate successful calvarial reossification is well described. This capacity is markedly attenuated in mature animals and in humans greater than 2 years of age. Previous studies have implicated the dura mater as critical to successful calvarial reossification. The au...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:

    authors: Greenwald JA,Mehrara BJ,Spector JA,Fagenholz PJ,Saadeh PB,Steinbrech DS,Gittes GK,Longaker MT

    更新日期:2000-09-01 00:00:00

  • Soft-tissue augmentation of the middle and lower face using the deepithelialized submental flap.

    abstract:BACKGROUND:Facial contour augmentation is an entity often encountered by reconstructive surgeons. To date, very different autologous tissues such as fat, dermofat, dermal fascia, muscle, cartilage, and bone in the manner of grafts or flaps according to the requirements of the defect have been used for facial augmentati...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000252002.76466.cf

    authors: Tan O,Atik B,Parmaksizoglu D

    更新日期:2007-03-01 00:00:00

  • Rigid internal fixation of fractures in the angular region of the mandible: an analysis of factors contributing to different complications.

    abstract::Data relating to 113 patients with 121 mandibular angle fractures treated according to the principles of rigid internal fixation were analyzed to determine which clinical factors are associated with different complications. Certain clinical characteristics were found to be associated with major complications. The use ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:

    authors: Iizuka T,Lindqvist C

    更新日期:1993-02-01 00:00:00

  • Dermal substitution in acute burns and reconstructive surgery: a subjective and objective long-term follow-up.

    abstract::Tissue engineering and dermal substitution are currently prominent topics of wound-healing research. However, no extensive clinical trials with objective evaluation criteria have been published so far that support the clinical effectiveness of dermal equivalents in the long term. The dermal substitute that is discusse...

    journal_title:Plastic and reconstructive surgery

    pub_type: 临床试验,杂志文章

    doi:10.1097/00006534-200112000-00014

    authors: van Zuijlen PP,Vloemans JF,van Trier AJ,Suijker MH,van Unen E,Groenevelt F,Kreis RW,Middelkoop E

    更新日期:2001-12-01 00:00:00

  • The foreign body response: at the interface of surgery and bioengineering.

    abstract:BACKGROUND:The surgical implantation of materials and devices has dramatically increased over the past decade. This trend is expected to continue with the broadening application of biomaterials and rapid expansion of aging populations. One major factor that limits the potential of implantable materials and devices is t...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审

    doi:10.1097/PRS.0000000000001193

    authors: Major MR,Wong VW,Nelson ER,Longaker MT,Gurtner GC

    更新日期:2015-05-01 00:00:00

  • Breast reconstruction following nipple-sparing mastectomy: a systematic review of the literature with pooled analysis.

    abstract:BACKGROUND:Nipple-sparing mastectomy is a controversial option for breast cancer treatment due to locoregional recurrence and distant metastasis. In addition to these oncologic factors, technical factors such as ideal incision type or reconstructive options are also debatable. This systematic review examines current tr...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审

    doi:10.1097/PRS.0b013e3182a48b8a

    authors: Endara M,Chen D,Verma K,Nahabedian MY,Spear SL

    更新日期:2013-11-01 00:00:00

  • Standardization in photography for body contour surgery and suction-assisted lipectomy.

    abstract::Despite the existence of basic clinical standards in plastic surgery, specific guidelines for body contour photography have not been detailed previously. In this report we propose standard and supplemental views for positioning of the subject for suction-assisted lipectomy and body contour surgery. Also demonstrated a...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199707000-00034

    authors: Gherardini G,Matarasso A,Serure AS,Toledo LS,DiBernardo BE

    更新日期:1997-07-01 00:00:00

  • The psychic consequences of a reduction mammaplasty.

    abstract::We studied 8 consecutive patients who had reduction mammaplasties to determine their psychological reactions. All 8 experienced pleasure with the operative results and the improvement or disappearance of their physical symptoms. Most described a general increase in self-esteem. In 5 of the 8 patients, however, there w...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:

    authors: Goin MK,Goin JM,Gianini MH

    更新日期:1977-04-01 00:00:00

  • Free-flap free flap.

    abstract::We present a patient who sustained bilateral below-knee amputations that were treated with skin grafts as initial coverage. A latissimus dorsi free flap was later used as definitive coverage of one stump. Then at a subsequent operation a portion of the same latissimus dorsi free flap was reharvested, again as a free f...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198907000-00025

    authors: Valauri FA,Alpert BS,Buncke HJ

    更新日期:1989-07-01 00:00:00

  • Factors Associated with Primary and Secondary Amputation following Limb-Threatening Upper Extremity Trauma.

    abstract:BACKGROUND:The aim of this study was to assess the factors associated with primary and secondary amputation in patients with limb-threatening trauma to the upper extremity, describe the incidence of these injuries, and describe the procedures involved in the treatment of these difficult injuries. METHODS:A retrospecti...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1097/PRS.0000000000006644

    authors: Nawijn F,Westenberg RF,Langhammer CG,Chen NC,Eberlin KR

    更新日期:2020-04-01 00:00:00

  • Basal cell carcinoma in the groin of a black patient with femoral vessel invasion and a second primary malignancy.

    abstract::We have presented an unusual case of basal cell carcinoma that presented in a 76-year-old black woman. This is an atypical case in that it occurred in a black patient, and the incidence of basal cell carcinoma is low in black people. The lesion was located in the groin, which is an uncommon location for basal cell car...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199610000-00022

    authors: Beckenstein MS,Windle BH

    更新日期:1996-10-01 00:00:00

  • Analysis of the lengthening effect of the muscle repair in functional cleft lip repair.

    abstract::In 14 patients undergoing functional cleft lip repair, changes in the lengths of the key lip segments were measured preoperatively, after the muscle layer was repaired, and after the skin was repaired using pieces of wire bent to follow the curves of the lip in three dimensions. The cleft side of the lip was shorter t...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198810000-00006

    authors: Dado DV

    更新日期:1988-10-01 00:00:00

  • Surgical techniques for a deep concha, a pseudomeatus, and high projection in congenital microtia.

    abstract::Some modified surgical techniques are described for constructing a deep conchal cavity and pseudomeatus and obtaining high auricular projection in congenital microtia. At the primary operation, a rather small portion of the microtic vestige is utilized for the lobule by switching, sparing the skin for the concha, with...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198604000-00004

    authors: Isshiki N,Koyama H,Suzuki S,Taira T

    更新日期:1986-04-01 00:00:00

  • The "knot twist": a new method of securing wire loops--an assessment of mechanical properties.

    abstract::A model simulating intraosseous wire fixation was used to assess different methods of securing wire loops. Square knotting, twisting, and a new method, the "knot twist," were assessed. The importance of anchoring the butt ends of twisted wire is emphasized. A new, convenient technique of wire fixation is described. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:

    authors: Green RA,Zins JE

    更新日期:1988-06-01 00:00:00

  • Neurovascular free flaps. A preliminary report.

    abstract::An experimental, anatomical, and clinical study has confirmed the feasibility of free neurovascular flaps. The interrelationship between the cutaneous vascular and neural supply has been defined and demonstrated electrophysiologically, both in man and in experimental animals. A potential donor site in man has been e...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-197507000-00004

    authors: Daniel RK,Terzis J,Schwarz G

    更新日期:1975-07-01 00:00:00

  • The association between breast surgeons' attitudes toward breast reconstruction and their reconstruction-related information-giving behaviors: a nationwide survey in Japan.

    abstract:BACKGROUND:Breast reconstruction after breast cancer surgery has come to be regarded as an achievable treatment option to improve patients' quality of life. Although it has been pointed out that surgeons' information-giving behaviors affect women's decision to undergo breast reconstruction surgery, little is known abou...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000233149.36751.d2

    authors: Takahashi M,Kai I,Hisata M,Higashi Y

    更新日期:2006-12-01 00:00:00

  • Cheng's method for reconstruction of a functionally sensitive penis.

    abstract::This article introduces a new surgical method for one-stage reconstruction of the penis. It is applied to the reconstruction of the microphallus as well as to traumatic cases with the residual stump of the amputated penis not less than 3 cm long. By transferring the original glans or the residual penile stump to the a...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199701000-00012

    authors: Cheng KX,Zhang RH,Zhou S,Jiang KC,Eid AE,Huang WY

    更新日期:1997-01-01 00:00:00

  • The vascular anatomy of the inner anterior abdominal wall with special reference to the transversus and rectus abdominis musculoperitoneal (TRAMP) composite flap for vaginal reconstruction.

    abstract::This study was designed to clarify the vascularization of the inner anterior abdominal wall with respect to the novel transversus and rectus abdominis musculoperitoneal (TRAMP) flap, which was introduced recently for vaginal reconstruction. A series of human cadavers was injected with a lead oxide-gelatine mixture by ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199703000-00016

    authors: Konerding MA,Gaumann A,Shumsky A,Schlenger K,Hockel M

    更新日期:1997-03-01 00:00:00

  • Anatomical localization of the umbilicus: an Indian study.

    abstract:BACKGROUND:The umbilicus is essential to the aesthetic appearance of the abdomen. However, very little has been written about the exact location of the aesthetically pleasing umbilicus as far as the Indian cosmopolitan population is concerned. Although a variety of reconstructive methods are available, no real standard...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000204793.70787.42

    authors: Abhyankar SV,Rajguru AG,Patil PA

    更新日期:2006-04-01 00:00:00

  • Evidence-based medicine: Unilateral cleft lip and nose repair.

    abstract:LEARNING OBJECTIVES:After reading this article, the participant should be able to: 1. Describe the anatomical malformations found in unilateral cleft lip deformity. 2. Discuss current methods of measuring the deformity and subsequent outcomes. 3. Discuss preoperative assessments, workup, and the use of early interventi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审

    doi:10.1097/PRS.0000000000000721

    authors: Greives MR,Camison L,Losee JE

    更新日期:2014-12-01 00:00:00

  • Rapid three-dimensional measuring system for facial surface structure.

    abstract::A noncontact three-dimensional measuring system (liquid crystal range finder system) is described. Three-dimensional facial surface data (more than 30,000 points) could be obtained in 1 second, and the resolution was approximately 0.4 mm. The reliability and repeatability of the results were validated with a calibrati...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199811000-00046

    authors: Yamada T,Sugahara T,Mori Y,Sakuda M

    更新日期:1998-11-01 00:00:00

  • The Effect of Abdominoplasty and Outcome of Rectus Fascia Plication on Health-Related Quality of Life in Post-Bariatric Surgery Patients.

    abstract:BACKGROUND:The authors prospectively evaluated the effect of isolated abdominoplasty with or without rectus fascia plication on the physical, functional, and psychosocial dimensions of health-related quality of life in post-bariatric surgery patients. They also evaluated whether the objectively measured amount of abdom...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/PRS.0000000000001772

    authors: Staalesen T,Olsén MF,Elander A

    更新日期:2015-12-01 00:00:00

  • Perfusion Bioreactor Culture of Bone Marrow Stromal Cells Enhances Cranial Defect Regeneration.

    abstract:BACKGROUND:Cell-seeded biomaterial scaffolds have been proposed as a future option for reconstruction of bone tissue. The ability to generate larger, functional volumes of bone has been a challenge that may be addressed through the use of perfusion bioreactors. In this study, the authors investigated use of a tubular p...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000005529

    authors: Gandhi JK,Kao SW,Roux BM,Rodriguez RA,Tang SJ,Fisher JP,Cheng MH,Brey EM

    更新日期:2019-05-01 00:00:00

  • Mesh lift: a new procedure for long-lasting results in brow lift surgery.

    abstract:BACKGROUND:Prevention of recurrent eyebrow ptosis is the greatest challenge in brow lift surgery today. In this article, the author describes a new surgical procedure to provide long-lasting results in brow lift surgery. METHODS:Over 7 years, this new technique was used in 37 patients. Except for four, all patients we...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000182601.94805.58

    authors: Mutaf M

    更新日期:2005-10-01 00:00:00

  • The forearm flap.

    abstract::We present our experimental and clinical experiences with the free neurovascular forearm flap. The flap is based on the radial artery, one of the great veins of the forearm (cephalic, basilic, or interconnecting vein), and one or two cutaneous forearm nerves (ulnar, median, or lateral). Because of the standard anatomy...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198209000-00007

    authors: Mühlbauer W,Herndl E,Stock W

    更新日期:1982-09-01 00:00:00

  • Functional reconstruction of a bilateral maxillectomy defect using a fibula osteocutaneous flap with osseointegrated implants.

    abstract::We have achieved functional reconstruction for a bilateral upper alveolar bone, gingival, and palatal defect that has various problems originating from instability of the prosthesis using the fibula osteocutaneous flap with osseointegrated implants. The flap had three bone segments and two skin paddles. The combined b...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199510000-00032

    authors: Nakayama B,Matsuura H,Ishihara O,Hasegawa H,Mataga I,Torii S

    更新日期:1995-10-01 00:00:00

  • The location of the blood supply in random flaps.

    abstract::The blood supply of random skin flaps in the pig is mainly in a microvascular plexus in the deeper layer of the dermis. However, there is vasculature in both the dermis and the subcutaneous fat, with good collaterals between the two systems. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-197609000-00010

    authors: Myers B,Donovan W

    更新日期:1976-09-01 00:00:00

  • Have we found an ideal soft-tissue flap? An experience with 672 anterolateral thigh flaps.

    abstract::The free anterolateral thigh flap is becoming one of the most preferred options for soft-tissue reconstruction. Between June of 1996 and August of 2000, 672 anterolateral thigh flaps were used in 660 patients at Chang Gung Memorial Hospital. Four hundred eighty-four anterolateral thigh flaps were used for head and nec...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-200206000-00007

    authors: Wei FC,Jain V,Celik N,Chen HC,Chuang DC,Lin CH

    更新日期:2002-06-01 00:00:00

  • Camouflaging hairless areas on the male face by artistic tattoo.

    abstract::Marked improvement in the aesthetic appearance of scars and skin grafts of the hair-bearing facial areas of male patients can be achieved by camouflage with punctate tattooing. The technique is easy and simple to learn. The procedure is performed under local anesthesia and on an outpatient basis. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199308000-00027

    authors: Tsur H,Kaplan HY

    更新日期:1993-08-01 00:00:00

  • Early Results Using Sterilized Acellular Human Dermis (Neoform) in Post-Mastectomy Tissue Expander Breast Reconstruction.

    abstract::Acellular dermal products play a beneficial role in immediate tissue expander breast reconstruction. They provide improved coverage and support in the lower pole, allowing the pectoralis muscle to drape over most of the expander and maximize lower pole expansion. Tissue incorporation is desired without any adverse aff...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e31819c4337

    authors: Losken A

    更新日期:2009-06-01 00:00:00