Abstract:
:Fluorescent probes have been applied to study by epifluorescence microscopy interactions between species in an Anabaena flos-aquae association, derived from a freshwater community. Glycoconjugates (macromolecules containing carbohydrate residues) have been shown to play a vital role in recognition processes and in formation of attachment sites between cells. By use of a group of proteins (lectins) with specific affinities to certain sugar residues we were able to clucidate specific patterns expressed at cellular surfaces, thereby indicating the physiological state of a cell. Low molecular weight fluorochromes, like the fluorescent brightener calcofluor white have been shown to be extremely useful tools for visualizing structural entities (and species) which otherwise escape notice by microscopy. We have demonstrated that the decline of the bloom of Anabaena flos-aquae was caused or at least accelerated by action of a fungus (chytrid). We also proved that the shell (lorica) of a choanoflagellate most likely is built from chitin.
journal_name
Oecologiajournal_title
Oecologiaauthors
Müller U,Sengbusch PVdoi
10.1007/BF00399219subject
Has Abstractpub_date
1983-05-01 00:00:00pages
215-219issue
2eissn
0029-8549issn
1432-1939pii
10.1007/BF00399219journal_volume
58pub_type
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