Abstract:
BACKGROUND:Adipocyte viability is affected by fat preparation and processing methods, but rigorous and objective studies of these relationships are lacking. OBJECTIVES:The authors conducted a comprehensive evaluation of variables affecting adipocyte viability prior to injection of fat at the recipient site. METHODS:Lipoaspirates from 48 patients were processed by high or low vacuum pressure, decantation, electric or manual centrifugation, concentration with cotton gauze, washing, repeated syringe transfer, exposure to lidocaine, and exposure to air. The effects of these variables on adipocyte viability in vitro were ascertained with the MTT assay. The influences of patient obesity (ie, a body mass index [BMI] >30 kg/m2) and enrichment with stem cells on adipocyte viability also were determined. RESULTS:High vacuum pressure decreased adipocyte viability. Decantation yielded the highest cell viability, followed by washing, concentration with cotton gauze, and centrifugation. Exposure to concentrated lidocaine ambient air exposure, and transfer between syringes significantly decreased viability. Patient obesity was predictive of lower adipocyte viability regardless of processing method, whereas stem cell enrichment significantly improved viability (P < .0001). CONCLUSIONS:To maximize adipocyte viability, fat should be obtained with dilute local anesthetics and low vacuum pressure, and the lipoaspirate should be maintained in a closed system. To clear cellular debris and blood, the lipoaspirate should be prepared by washing, and the fat should be enriched with adipose stem cells. Decreased adipocyte viability should be expected when fat is harvested from patients with high BMIs.
journal_name
Aesthet Surg Jjournal_title
Aesthetic surgery journalauthors
Cucchiani R,Corrales Ldoi
10.1093/asj/sjw106subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1164-1173issue
10eissn
1090-820Xissn
1527-330Xpii
sjw106journal_volume
36pub_type
杂志文章abstract:BACKGROUND:Transaxillary subpectoral augmentation mammaplasty (TASPA) is not widely accepted due to perceived limitations in pocket access, visualization, control, and subsequent risk for postoperative complications. Current published data do little to substantiate or refute those claims. OBJECTIVE:A retrospective cha...
journal_title:Aesthetic surgery journal
pub_type: 杂志文章
doi:10.1177/1090820X11416936
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abstract:BACKGROUND:Although the anatomy of the individual pectoralis major and minor muscles has been described previously, never before has the anatomic relationship between these muscles been investigated. OBJECTIVE:The authors identify the anatomic relationship of the costal origins of the pectoralis major and minor muscle...
journal_title:Aesthetic surgery journal
pub_type: 杂志文章
doi:10.1177/1090820X13519643
更新日期:2014-02-01 00:00:00
abstract::Full and high cheekbones are considered a desirable component of facial attractiveness. The aging process can result in a loss of facial volume and changes in facial contours. Cheek augmentation can replace lost facial volume, rejuvenate facial appearance, enhance cheek prominence, and improve facial symmetry. A new, ...
journal_title:Aesthetic surgery journal
pub_type: 杂志文章
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abstract:Background:Seroma is the most common complication of abdominoplasty. Certain modifications to abdominoplasty may reduce the risk of seroma. Objectives:The authors evaluated the incidence of seroma for 3 abdominoplasty techniques: preservation of Scarpa's fascia, placement of progressive tension (or quilting) sutures, ...
journal_title:Aesthetic surgery journal
pub_type: 杂志文章,meta分析
doi:10.1093/asj/sjx051
更新日期:2017-10-16 00:00:00
abstract::Efforts to improve lipoplasty results through the application of focused energy continue to arouse interest. The author's initial experience with power-assisted lipoplasty indicates that this technique is safe, fast, and capable of producing results equal or superior to those achieved through use of traditional lipopl...
journal_title:Aesthetic surgery journal
pub_type: 杂志文章
doi:10.1067/maj.2001.113641
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journal_title:Aesthetic surgery journal
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更新日期:2010-05-01 00:00:00
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journal_title:Aesthetic surgery journal
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journal_title:Aesthetic surgery journal
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更新日期:2013-07-01 00:00:00
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章
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更新日期:2021-01-25 00:00:00
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journal_title:Aesthetic surgery journal
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更新日期:2006-05-01 00:00:00
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Aesthetic surgery journal
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章
doi:10.1016/j.asj.2006.01.001
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:In 1971, Ribeiro isolated a segment in the inferior pole of the ptotic breast, nourished by muscular perforating vessels, and moved it cranially to the posterior region of the remaining detached breast tissue, where it was fixed to the pectoral fascia. This maneuver created a flap with autologous implant fun...
journal_title:Aesthetic surgery journal
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更新日期:2020-01-01 00:00:00
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journal_title:Aesthetic surgery journal
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更新日期:2016-02-01 00:00:00
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journal_title:Aesthetic surgery journal
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更新日期:2012-07-01 00:00:00
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journal_title:Aesthetic surgery journal
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doi:10.1093/asj/sjz348
更新日期:2020-07-13 00:00:00
abstract::Clinical trials, retrospective studies, and case studies indicate that botulinum toxin type A is safe and effective for prophylactic and acute treatment of migraine headache. Further studies are needed to scientifically determine the optimum dosage and administration sites, and which patients are most likely to benefi...
journal_title:Aesthetic surgery journal
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doi:10.1067/maj.2002.121958
更新日期:2002-01-01 00:00:00
abstract::Injectable synthetic hyaluronic acid is biodegradable and biocompatible with human hyaluronic acid. Soft tissue augmentation with this product does not require prior skin testing and provides robust augmentation. Its ability to attract and maintain significant amounts of water accounts for the volume and duration of c...
journal_title:Aesthetic surgery journal
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doi:10.1016/j.asj.2004.04.009
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Studies have shown that scarring is a primary reason for patient dissatisfaction with reduction mammaplasty. OBJECTIVES:The authors prospectively evaluated patient preferences for the 3 most common breast reduction scar patterns: horizontal, vertical, and Wise. METHODS:Sixty-six patients were recruited for...
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更新日期:2013-08-01 00:00:00
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journal_title:Aesthetic surgery journal
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章,随机对照试验
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更新日期:2008-07-01 00:00:00
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journal_title:Aesthetic surgery journal
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journal_title:Aesthetic surgery journal
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章
doi:10.1016/j.asj.2005.05.010
更新日期:2005-07-01 00:00:00
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章
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更新日期:2019-08-22 00:00:00
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
abstract:Background:Lower eyelid blepharoplasty has continued to evolve with ongoing debate regarding optimal techniques. Despite large case series publishing excellent results and minimal complications, the true longevity of these procedures remains unclear. Objectives:The aim of this study was to determine how thoroughly the...
journal_title:Aesthetic surgery journal
pub_type: 历史文章,杂志文章,评审
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更新日期:2018-11-12 00:00:00
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journal_title:Aesthetic surgery journal
pub_type: 杂志文章
doi:10.1016/j.asj.2008.07.010
更新日期:2008-09-01 00:00:00