Abstract:
:Putrescine is widely used in industrial production of bioplastics, pharmaceuticals, agrochemicals, and surfactants. Although engineered Corynebacterium glutamicum has been successfully used to produce high levels of putrescine, the overall cellular physiological and metabolic changes caused by overproduction of putrescine remains unclear. To reveal the transcriptional changes that occur in response to putrescine production in an engineered C. glutamicum strain, a comparative transcriptomic analysis was carried out. Overproduction of putrescine resulted in transcriptional downregulation of genes involved in glycolysis; the TCA cycle, pyruvate degradation, biosynthesis of some amino acids, oxidative phosphorylation; vitamin biosynthesis (thiamine and vitamin 6), metabolism of purine, pyrimidine and sulfur, and ATP-, NAD-, and NADPH-consuming enzymes. The transcriptional levels of genes involved in ornithine biosynthesis and NADPH-forming related enzymes were significantly upregulated in the putrescine producing C. glutamicum strain PUT-ALE. Comparative transcriptomic analysis provided some genetic modification strategies to further improve putrescine production. Repressing ATP- and NADPH-consuming enzyme coding gene expression via CRISPRi enhanced putrescine production.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Li Z,Liu JZdoi
10.3389/fmicb.2017.01987subject
Has Abstractpub_date
2017-10-17 00:00:00pages
1987issn
1664-302Xjournal_volume
8pub_type
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journal_title:Frontiers in microbiology
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2017.01350
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2020-11-05 00:00:00
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
doi:10.3389/fmicb.2018.03101
更新日期:2018-12-12 00:00:00
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2020.572314
更新日期:2020-09-17 00:00:00
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更新日期:2018-09-05 00:00:00
abstract::[This corrects the article DOI: 10.3389/fmicb.2018.03247.]. ...
journal_title:Frontiers in microbiology
pub_type: 已发布勘误
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pub_type: 杂志文章,评审
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更新日期:2019-03-11 00:00:00