Abstract：
:The effects of C-phycocyanin (C-pc), a phycobiliprotein, on the expression of pro-fibrotic mediators in hyper-tropic scarring such as connective tissue growth factor (CTGF) and α-smooth muscle actins (α-SMA) were investigated in relation to trans-differentiation of fibroblast to myo-fibroblast, an icon of scar formation. C-pc was isolated from Spirulina Platensis extract using sonication method and C-pc concentration was determined by Bennet and Bogorad equation. α-SMA and CTGF levels in wounded primary human dermal fibroblasts were determined by western blot analysis and immuno-fluorescence confocal microscope was employed. Fibroblast contractility was examined by three-dimensional collagen lattice contraction assay. There was an elevation of α-SMA (121%) and CTGF (143%) levels in wound cells as compared with non-wound cells. The does-response profiles of down regulation demonstrated that the maximum inhibitions of α-SMA by 63% (p<0.05) and CTGF by 50% (p<0.1) were achieved by C-pc (6 nM) treated cells. In confocal assay, non-wound fibroblasts exhibited basal level of α-SMA staining, while wounded cells without C-pc treatment showed strong up-regulation of α-SMA by 147% (p<0.05). C-pc (6 nM) inhibited α-SMA expression by 70% (p<0.05) and reduced collagen contraction by 29% (p<0.05). C-pc seemed to lessen the over expression of CTGF, α-SMA, subsequently alleviating the fibrotic contracture. This study suggests the potential application of C-pc to regulation of the expression of pro-fibrotic mediators in scarring process and its potential usage as an efficient means for anti-fibrosis therapy.
journal_name
Tissue Eng Regen Medjournal_title
Tissue engineering and regenerative medicineauthors
An E,Park H,Lee ACdoi
10.1007/s13770-015-0104-5subject
Has Abstractpub_date
2016-02-06 00:00:00pages
388-395issue
4eissn
1738-2696issn
2212-5469pii
104journal_volume
13pub_type
杂志文章abstract：:We aim to examine the influence of platelet rich plasma (PRP) and spatial cues in cartilage/bone matrix forming proteins, and to evaluate the mitotic and chemotactic potential of PRP on human mesenchymal stem cells (hMSCs). Directed cell migration towards PRP gradients was assessed in chemotactic chambers, and recorde...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0062-1
更新日期：2017-06-23 00:00:00
abstract：:[This corrects the article DOI: 10.1007/s13770-017-0096-4.]. ...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,已发布勘误
doi：10.1007/s13770-017-0111-9
更新日期：2018-01-19 00:00:00
abstract：:The author would like to correct the names for the below co-authors in the online published article. ...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,已发布勘误
doi：10.1007/s13770-020-00251-x
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Regeneration of soft tissue defects is essential for adipose tissue pathologies and disease, trauma, or injury-induced damage. Here, we show that umbilical cord blood-derived mesenchymal stem cells could potentially be tailored and used for the reconstruction of specific damaged sites. Adipogenesis can be ex...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-019-00237-4
更新日期：2020-04-01 00:00:00
abstract：:In almost all human tissues and organs, adult stem cells or tissue stem cells are present in a unique location, the so-called stem cell niche or its equivalent, continuously replenishing functional differentiated cells. Those endogenous stem cells can be expanded for cell therapeutics using ex vivo cell culture or rec...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0097-3
更新日期：2017-11-20 00:00:00
abstract：Background:The liver is an organ with remarkable regenerative capacity; however, once chronic fibrosis occurs, liver failure follows, with high mortality and morbidity rates. Continuous exposure to proinflammatory stimuli exaggerates the pathological process of liver failure; therefore, immune modulation is a potential...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0160-8
更新日期：2018-10-19 00:00:00
abstract：BACKGROUND:Fetal bovine serum is widely used as a growth supplement for cell culture medium; however, animal-borne pathogens increase the risk of transmitting infectious agents. Platelet-rich fibrin is recently considered as a successful alternative but leukocytes present limits to its allogeneic feasibility. The aim o...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-020-00279-z
更新日期：2020-08-01 00:00:00
abstract：BACKGROUND:Recent evidence from in vitro and in vivo studies indicates that bisphosphonates may promote osteoblastic bone formation and potently inhibit osteoclast activity. However, little is known about the potential effect of bisphosphonates on the recruitment of osteoblastic precursors from patient-derived bone mar...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0127-9
更新日期：2018-06-08 00:00:00
abstract：:Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Po...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-015-0029-z
更新日期：2016-02-02 00:00:00
abstract：:Reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) enables the possibility of generating patient-specific cells. However, the low efficiency issue associated with iPSCs generation has limited iPSCs usage in research and clinical applications. In this study, we developed a high efficiency sy...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-0015-0
更新日期：2016-12-17 00:00:00
abstract：BACKGROUND:Different methods have been used to inject stem cells into the eye for research. We previously explored the intravitreal route. Here, we investigate the efficacy of intravenous and subretinal-transplanted human dental pulp stem cells (DPSCs) in rescuing the photoreceptors of a sodium iodate-induced retinal d...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-020-00312-1
更新日期：2021-01-07 00:00:00
abstract：Background:Provision of optimal endometrial stromal cells is essential in uterine tissue engineering. Culture of these cells is significantly influenced by gonadotropin hormones. This investigation attempted to define the proliferation profiles of murine uterine endometrial stromal cells during in vitro culture with re...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0156-4
更新日期：2018-08-28 00:00:00
abstract：:The MTT assay showed that the cell proliferation on hydroxyapatite (HAp) and HAp/bone morphogenic protein (BMP) coated group was better than the control and BMP coated groups at 5 days. And after 7 days of culture, the mRNA expression levels of type I collagen, osteonectin, osteopontin, bonesialoprotein, BMP-2, alkali...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9051-z
更新日期：2016-06-09 00:00:00
abstract：:Deformities in tissues and organs can be treated by using tissue engineering approach offering the development of biologically functionalized scaffolds from a variety of polymer blends which mimic the extracellular matrix and allow adjusting the material properties to meet the defect architecture. In recent years, res...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9092-3
更新日期：2016-10-20 00:00:00
abstract：:Tissue-resident stem cells are surrounded by a microenvironment known as 'stem cell niche' which is specific for each stem cell type. This niche comprises of cell-intrinsic and -extrinsic factors like biochemical and biophysical signals, which regulate stem cell characteristics and differentiation. Biochemical signals...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-020-00301-4
更新日期：2020-11-24 00:00:00
abstract：BACKGROUND:Skeletal muscle injuries are frequent clinical challenges due to associated fibrosis and disability. Regenerative medicine is an emerging promising strategy for such cases. The aim of this study was to compare between the effects of bone marrow-mesenchymal stem cells (BM-MSCs) versus adipose tissue stromal c...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-020-00288-y
更新日期：2020-12-01 00:00:00
abstract：:Healthy and high quality of life has become the main issue with increasing human life span. Many biological treatments for osteoarthritis of the knee have been tried with limited success. We compared data from 7 patients who underwent total knee arthroplasty and 46 patients who underwent autologous bone-marrow mesench...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9125-y
更新日期：2016-04-05 00:00:00
abstract：BACKGROUND:Peritendinous adhesion that simultaneous with tendon healing link the healing tendon to the surrounding tissue. It results in functional disability, and has a significant adverse impact on health as well as social and economic development. METHODS:Based on a search in the PubMed and Web of Science database,...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-020-00300-5
更新日期：2020-11-04 00:00:00
abstract：:Dental pulp stem cells (DPSCs) are mesenchymal stem cells with high self-renewal potential that have the ability to differentiate into several cell types. Thus, DPSCs have become a promising source of cells for several applications in regenerative medicine, tissue engineering, and stem cell therapy. Numerous methods h...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-017-0036-3
更新日期：2017-03-14 00:00:00
abstract：:The implantation of bioengineered scaffolds into lesion-induced gaps of the spinal cord is a promising strategy for promoting functional tissue repair because it can be combined with other intervention strategies. Our previous investigations showed that functional improvement following the implantation of a longitudin...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9115-0
更新日期：2016-10-20 00:00:00
abstract：:Both mature and stem cell-derived hepatocytes lost their phenotype and functionality under conventional culture conditions. However, the 3D scaffolds containing the main extracellular matrix constitutions, such as heparin, may provide appropriate microenvironment for hepatocytes to be functional. The current study aim...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0048-z
更新日期：2017-05-23 00:00:00
abstract：:Human dermal fibroblast is essential in wound healing of the skin through the synthesis of extracellular matrix proteins. With respect to oxidative stress, the effects of remifentanil on human dermal fibroblast have received little attention. Therefore, we investigated the effects of remifentanil on the apoptosis and ...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0030-9
更新日期：2017-03-02 00:00:00
abstract：:A titanium implant surface when coated with biodegradable, highly porous, osteogenic nanofibrous coating has shown enhanced intrinsic osteoinductive and osteoconductive properties. This coating mimics extracellular matrix resulting in differentiation of stem cells present in the peri-implant niche to osteoblast and he...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0106-6
更新日期：2018-01-19 00:00:00
abstract：Background:Biopolymeric in situ hydrogels play a crucial role in the regenerative repair and replacement of infected or injured tissue. They possess excellent biodegradability and biocompatibility in the biological system, however only a few biopolymeric in situ hydrogels have been approved clinically. Researchers have...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-018-0159-1
更新日期：2018-09-14 00:00:00
abstract：Background:Osteoarthritis (OA), the most common arthritis, is one of the most frequently encountered orthopaedic conditions. As a small number of large joints such as knee and hip are affected in OA, OA is an ideal target for local therapy. Although corticosteroid and hyaluronic acid have been traditionally used for jo...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-018-00176-6
更新日期：2019-01-23 00:00:00
abstract：:Stem cell technologies are particularly attractive in Parkinson's disease (PD) research although they occasionally need long-term treatment for anti-parkinsonian activity. Unfortunately, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) widely used as a model for PD has several limitations, including the risk of dos...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-015-0106-3
更新日期：2015-12-28 00:00:00
abstract：:Several barriers such as gastric pH, enzymatic degradation and rapid transit should be overcome to orally deliver antigens for taking up by epithelial microfold cells in Peyer's patches of small intestine. To solve the above mentioned problems, we designed pH-sensitive and mucoadhesive polymeric microparticles (MPs) p...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0082-x
更新日期：2017-10-17 00:00:00
abstract：BACKGROUND:Hematopoietic stem/progenitor cells (HSPCs) have the property to return to the bone marrow, which is believed to be critical in situations such as HSPC transplantation. This property plays an important role in the stemness, viability, and proliferation of HSPCs, also. However, most in vitro models so far hav...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-019-00224-9
更新日期：2020-02-01 00:00:00
abstract：BACKGROUND:Three-dimensional (3D) cell cultures with architectural and biomechanical properties similar to those of natural tissue have been the focus for generating liver tissue. Microarchitectural organization is believed to be crucial to hepatic function, and 3D cell culture technologies have enabled the constructio...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-020-00245-9
更新日期：2020-12-01 00:00:00
abstract：:[This corrects the article DOI: 10.1007/s13770-016-0016-z.]. ...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,已发布勘误
doi：10.1007/s13770-017-0072-z
更新日期：2017-07-26 00:00:00
