Abstract：
:Stem cell technologies are particularly attractive in Parkinson's disease (PD) research although they occasionally need long-term treatment for anti-parkinsonian activity. Unfortunately, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) widely used as a model for PD has several limitations, including the risk of dose-dependent mortality and the difficulty of maintenance of PD symptoms during the whole experiment period. Therefore, we tested if our novel MPTP regimen protocol (2 mg/kg for 2 consecutive days and 1 mg/kg for next 3 consecutive days) can be maintained stable parkinsonism without mortality for long-term stem cell therapy. For this, we used small-bodied common marmoset monkeys (Callithrix jacchus) among several nonhuman primates showing high anatomical, functional, and behavioral similarities to humans. Along with no mortality, the behavioral changes involved in PD symptoms were maintained for 32 weeks. Also, the loss of jumping ability of the MPTP-treated marmosets in the Tower test was not recovered by 32 weeks. Positron emission tomography (PET) analysis revealed that remarkable decreases of bindings of 18F-FP-CIT were observed at the striatum of the brains of the marmosets received MPTP during the full period of the experiment for 32 weeks. In the substantia nigra of the marmosets, the loss of tyrosine hydroxylase (TH) immunoreactivity was also observed at 32 weeks following the MPTP treatment. In conclusion, our low-dose MPTP regimen protocol was found to be stable parkinsonism without mortality as evidenced by behavior, PET, and TH immunohistochemistry. This result will be useful for evaluation of possible long-term stem cell therapy for anti-parkinsonian activity.
journal_name
Tissue Eng Regen Medjournal_title
Tissue engineering and regenerative medicineauthors
Yun JW,Ahn JB,Kwon E,Ahn JH,Park HW,Heo H,Park JS,Kim H,Paek SH,Kang BCdoi
10.1007/s13770-015-0106-3subject
Has Abstractpub_date
2015-12-28 00:00:00pages
100-109issue
1eissn
1738-2696issn
2212-5469pii
106journal_volume
13pub_type
杂志文章abstract：:Deformities in tissues and organs can be treated by using tissue engineering approach offering the development of biologically functionalized scaffolds from a variety of polymer blends which mimic the extracellular matrix and allow adjusting the material properties to meet the defect architecture. In recent years, res...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9092-3
更新日期：2016-10-20 00:00:00
abstract：:The effects of C-phycocyanin (C-pc), a phycobiliprotein, on the expression of pro-fibrotic mediators in hyper-tropic scarring such as connective tissue growth factor (CTGF) and α-smooth muscle actins (α-SMA) were investigated in relation to trans-differentiation of fibroblast to myo-fibroblast, an icon of scar formati...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-015-0104-5
更新日期：2016-02-06 00:00:00
abstract：:The MTT assay showed that the cell proliferation on hydroxyapatite (HAp) and HAp/bone morphogenic protein (BMP) coated group was better than the control and BMP coated groups at 5 days. And after 7 days of culture, the mRNA expression levels of type I collagen, osteonectin, osteopontin, bonesialoprotein, BMP-2, alkali...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9051-z
更新日期：2016-06-09 00:00:00
abstract：:We investigated the utility of the duck-feet collagen extraction patching procedure in the traumatic tympanic membrane (TM) perforation via a comparison with spontaneous healing or paper patch. Fifty-six ears of adult male Sprague-Dawley rats, each weighing in the range of 250 to 300 g, were used for the animal studie...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0039-0
更新日期：2017-03-08 00:00:00
abstract：Background:Biopolymeric in situ hydrogels play a crucial role in the regenerative repair and replacement of infected or injured tissue. They possess excellent biodegradability and biocompatibility in the biological system, however only a few biopolymeric in situ hydrogels have been approved clinically. Researchers have...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-018-0159-1
更新日期：2018-09-14 00:00:00
abstract：:Several barriers such as gastric pH, enzymatic degradation and rapid transit should be overcome to orally deliver antigens for taking up by epithelial microfold cells in Peyer's patches of small intestine. To solve the above mentioned problems, we designed pH-sensitive and mucoadhesive polymeric microparticles (MPs) p...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0082-x
更新日期：2017-10-17 00:00:00
abstract：:Nerve regeneration after injury requires proper axon alignment to bridge the lesion site and myelination to achieve functional recovery. Transplanted scaffolds with aligned channels, have been shown to induce axon growth to some extent. However, the penetration of axons into the microchannels remain a challenge, influ...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0115-0
更新日期：2018-02-28 00:00:00
abstract：:In almost all human tissues and organs, adult stem cells or tissue stem cells are present in a unique location, the so-called stem cell niche or its equivalent, continuously replenishing functional differentiated cells. Those endogenous stem cells can be expanded for cell therapeutics using ex vivo cell culture or rec...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0097-3
更新日期：2017-11-20 00:00:00
abstract：:Healthy and high quality of life has become the main issue with increasing human life span. Many biological treatments for osteoarthritis of the knee have been tried with limited success. We compared data from 7 patients who underwent total knee arthroplasty and 46 patients who underwent autologous bone-marrow mesench...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9125-y
更新日期：2016-04-05 00:00:00
abstract：:Reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) enables the possibility of generating patient-specific cells. However, the low efficiency issue associated with iPSCs generation has limited iPSCs usage in research and clinical applications. In this study, we developed a high efficiency sy...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-0015-0
更新日期：2016-12-17 00:00:00
abstract：Background:Collagen organization within tissues has a critical role in wound regeneration. Collagen fibril diameter, arrangements and maturity between connective tissue growth factor (CTGF) small interfering RNA (siRNA) and mismatch scrambled siRNA-treated wound were compared to evaluate the efficacy of CTGF siRNA as a...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0166-2
更新日期：2018-11-14 00:00:00
abstract：:The implantation of bioengineered scaffolds into lesion-induced gaps of the spinal cord is a promising strategy for promoting functional tissue repair because it can be combined with other intervention strategies. Our previous investigations showed that functional improvement following the implantation of a longitudin...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-9115-0
更新日期：2016-10-20 00:00:00
abstract：Background:Mesenchymal stem cells (MSCs) have strong self-renewal ability and multiple differentiation potential. Some studies confirmed that spreading shape and area of single MSCs influence cell differentiation, but few studies focused on the effect of the circularity of cell shape on the osteogenic differentiation o...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-019-00213-y
更新日期：2019-08-29 00:00:00
abstract：BACKGROUND:Hematopoietic stem/progenitor cells (HSPCs) have the property to return to the bone marrow, which is believed to be critical in situations such as HSPC transplantation. This property plays an important role in the stemness, viability, and proliferation of HSPCs, also. However, most in vitro models so far hav...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-019-00224-9
更新日期：2020-02-01 00:00:00
abstract：:We aim to examine the influence of platelet rich plasma (PRP) and spatial cues in cartilage/bone matrix forming proteins, and to evaluate the mitotic and chemotactic potential of PRP on human mesenchymal stem cells (hMSCs). Directed cell migration towards PRP gradients was assessed in chemotactic chambers, and recorde...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0062-1
更新日期：2017-06-23 00:00:00
abstract：Background:The liver is an organ with remarkable regenerative capacity; however, once chronic fibrosis occurs, liver failure follows, with high mortality and morbidity rates. Continuous exposure to proinflammatory stimuli exaggerates the pathological process of liver failure; therefore, immune modulation is a potential...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0160-8
更新日期：2018-10-19 00:00:00
abstract：Background:Osteoarthritis (OA), the most common arthritis, is one of the most frequently encountered orthopaedic conditions. As a small number of large joints such as knee and hip are affected in OA, OA is an ideal target for local therapy. Although corticosteroid and hyaluronic acid have been traditionally used for jo...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-018-00176-6
更新日期：2019-01-23 00:00:00
abstract：:The author would like to correct the names for the below co-authors in the online published article. ...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,已发布勘误
doi：10.1007/s13770-020-00251-x
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Skeletal muscle injuries are frequent clinical challenges due to associated fibrosis and disability. Regenerative medicine is an emerging promising strategy for such cases. The aim of this study was to compare between the effects of bone marrow-mesenchymal stem cells (BM-MSCs) versus adipose tissue stromal c...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-020-00288-y
更新日期：2020-12-01 00:00:00
abstract：:Both mature and stem cell-derived hepatocytes lost their phenotype and functionality under conventional culture conditions. However, the 3D scaffolds containing the main extracellular matrix constitutions, such as heparin, may provide appropriate microenvironment for hepatocytes to be functional. The current study aim...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0048-z
更新日期：2017-05-23 00:00:00
abstract：:Spherical neural mass (SNM) is a mass of neural precursors that have been used to generate neuronal cells with advantages of long-term passaging capability with high yield, easy storage, and thawing. In this study, we differentiated neural retinal progenitor cells (RPCs) from human induced pluripotent stem cells (hiPS...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-016-0021-2
更新日期：2017-01-17 00:00:00
abstract：BACKGROUND:Different methods have been used to inject stem cells into the eye for research. We previously explored the intravitreal route. Here, we investigate the efficacy of intravenous and subretinal-transplanted human dental pulp stem cells (DPSCs) in rescuing the photoreceptors of a sodium iodate-induced retinal d...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-020-00312-1
更新日期：2021-01-07 00:00:00
abstract：Background:Provision of optimal endometrial stromal cells is essential in uterine tissue engineering. Culture of these cells is significantly influenced by gonadotropin hormones. This investigation attempted to define the proliferation profiles of murine uterine endometrial stromal cells during in vitro culture with re...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0156-4
更新日期：2018-08-28 00:00:00
abstract：:A titanium implant surface when coated with biodegradable, highly porous, osteogenic nanofibrous coating has shown enhanced intrinsic osteoinductive and osteoconductive properties. This coating mimics extracellular matrix resulting in differentiation of stem cells present in the peri-implant niche to osteoblast and he...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-017-0106-6
更新日期：2018-01-19 00:00:00
abstract：:Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Po...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-015-0029-z
更新日期：2016-02-02 00:00:00
abstract：BACKGROUND:Fetal bovine serum is widely used as a growth supplement for cell culture medium; however, animal-borne pathogens increase the risk of transmitting infectious agents. Platelet-rich fibrin is recently considered as a successful alternative but leukocytes present limits to its allogeneic feasibility. The aim o...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-020-00279-z
更新日期：2020-08-01 00:00:00
abstract：BACKGROUND:Recent evidence from in vitro and in vivo studies indicates that bisphosphonates may promote osteoblastic bone formation and potently inhibit osteoclast activity. However, little is known about the potential effect of bisphosphonates on the recruitment of osteoblastic precursors from patient-derived bone mar...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1007/s13770-018-0127-9
更新日期：2018-06-08 00:00:00
abstract：Background:For the bone-specific imaging, a structure-inherent targeting of bone tissue recently has been reported a new strategy based on incorporation of targeting moieties into the chemical structure of near-infrared (NIR) contrast agents, while conventional methods require covalent conjugation of bone-targeting lig...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-019-00208-9
更新日期：2019-08-19 00:00:00
abstract：:Tissue-resident stem cells are surrounded by a microenvironment known as 'stem cell niche' which is specific for each stem cell type. This niche comprises of cell-intrinsic and -extrinsic factors like biochemical and biophysical signals, which regulate stem cell characteristics and differentiation. Biochemical signals...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-020-00301-4
更新日期：2020-11-24 00:00:00
abstract：BACKGROUND:Peritendinous adhesion that simultaneous with tendon healing link the healing tendon to the surrounding tissue. It results in functional disability, and has a significant adverse impact on health as well as social and economic development. METHODS:Based on a search in the PubMed and Web of Science database,...
journal_title：Tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1007/s13770-020-00300-5
更新日期：2020-11-04 00:00:00
