Abstract:
:The aim of this work was to elucidate the role of the secondary metabolites produced by B. amyloliquefaciens BUZ-14 against B. cinerea, M. fructicola, M. laxa, P. digitatum, P. italicum and P. expansum both in vitro and in planta. The entire cell free supernatant (CFS) and the lipopeptide fraction (LPF) showed similar antifungal activities, completely inhibiting all the fungi at dilutions of 1:24 or even lower, whereas the non-butanolic fraction (NBF) barely inhibited the fungi. However, when the LPF and CFS were applied on fruit, only brown rot in peaches and blue rot in apples was totally inhibited. The main families of metabolites in the LPF were iturin A, fengycin and surfactin with maximum concentrations of 407, 853 and 658 μg mL-1, respectively. Subsequently, a TLC-bioautography revealed iturin A as the key metabolite in the inhibitions and allowed us to establish in vivo MICs of 16.9 and 33.9 μg mL-1 for Monilinia species and P. expansum, respectively. The application of 24 h-old BUZ-14 cultures suppressed brown rot in peaches and also blue rot in apples but failed to inhibit the other diseases. However, BUZ-14 was only able to grow and produce iturin A in peaches so we can deduce that the amount of iturin A brought with the cultures (36 ± 14 μg mL-1) could be enough to control both diseases. The strong antifungal activity of the iturin A present in the BUZ-14 CFS suggests that it could be successfully used for postharvest disease control. However, future research is necessary to maximize the iturin A production by B. amyloliquefaciens BUZ-14 in order to optimize a commercial application.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
Calvo H,Mendiara I,Arias E,Blanco D,Venturini MEdoi
10.1016/j.fm.2019.01.010subject
Has Abstractpub_date
2019-09-01 00:00:00pages
62-69eissn
0740-0020issn
1095-9998pii
S0740-0020(18)30717-2journal_volume
82pub_type
杂志文章abstract::Enhancement of nutritional or vitamin content of foods is commonly touted as a major benefit of probiotics. In this paper, we examined the ability of three probiotic bacteria either alone or in combination to enhance nutritional content. Pure and mixed cultures of Streptococcus thermophilus ST5 and Lactobacillus helve...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2010.06.003
更新日期:2010-10-01 00:00:00
abstract::The anti-listerial activity of microfloras from the surface of various smear-ripened cheeses was evaluated using four methods that were then compared. Method A measured the anti-listerial potential of supernatants from short-time liquid cultures, whereas in Method B, a model cheese was co-inoculated with the microflor...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2009.11.009
更新日期:2010-04-01 00:00:00
abstract::Clostridium perfringens type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe) are generally linked to food poisoning, while isolates carrying cpe on a plasmid (P-cpe) are associated with non-food-borne gastrointestinal diseases. Both C-cpe and P-cpe isolates can form metabolically dormant spores, which...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2017.06.020
更新日期:2018-02-01 00:00:00
abstract::Listeria monocytogenes continues to be one of the most important foodborne pathogens worldwide, either due to its incidence and/or to its high mortality rate. In the present study, a filtration-based protocol was applied for the screening of viable bacteria. Additionally, a complete method (enrichment, filtration, DNA...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2018.02.004
更新日期:2018-08-01 00:00:00
abstract::Kinetics of alcoholic fermentation by Saccharomyces cerevisiae wine strains in a synthetic medium with high sugar content were established for different nitrogen initial content and are presented for four strains. The composition of the medium was close to grape must except that the nitrogen source consisted mainly in...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2006.04.002
更新日期:2007-02-01 00:00:00
abstract::Molecular typing techniques are key tools in surveillance of food spoilage yeasts, in investigations on intra-species population diversity, and in tracing selected starters during fermentation. Unlike previous works on strain typing of Zygosaccharomyces spoilage species, here Zygosaccharomyces mellis and the Zygosacch...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2017.11.019
更新日期:2018-06-01 00:00:00
abstract::Cotija cheese is a Mexican handcrafted product made from raw cow milk whose ripening process occurs spontaneously and, presumably, it is influenced by environmental conditions. Its sensory characteristics and safety are probably the result of the balance between microbial populations and their metabolic capacity. In t...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2016.02.004
更新日期:2016-08-01 00:00:00
abstract::This study evaluated the effect of both gaseous and dissolved oxygen (O2) concentration (0 - 21%) on the growth of six heat-resistant moulds (HRMs) (Neosartorya and Byssochlamys spp.) previously isolated from high-acid fruit products. The study was performed in acidified potato dextrose agar (aPDA) with all six HRMs a...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2019.103243
更新日期:2019-12-01 00:00:00
abstract::Vibrio parahaemolyticus is recognized as the leading cause of human gastroenteritis associated with the consumption of seafood. The objective of this study was to model the growth kinetics of pathogenic and nonpathogenic V. parahaemolyticus in broth and oyster slurry. Primary growth models of V. parahaemolyticus in br...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2008.04.007
更新日期:2008-08-01 00:00:00
abstract::This work aimed to estimate the inactivation kinetic parameters of four potential beer spoilage bacteria (Lactobacillus brevis DSM 6235, Lactobacillus casei ATCC 334, Pediococcus damnosus DSM 20289 and Pediococcus damnosus ATCC 29358) inoculated in brewing yeast submitted to acid washing with purposes of yeast recycle...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2020.103513
更新日期:2020-10-01 00:00:00
abstract::Bacterial communities composition using 16S Next Generation Sequencing (NGS) and Volatile Organic Compounds (VOCs) profile of whole blue crabs (Callinectes sapidus) stored at 4 and 10 °C (proper and abuse temperature) simulating real storage conditions were performed. Conventional microbiological and chemical analyses...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2019.03.011
更新日期:2019-09-01 00:00:00
abstract::Shiga toxin-producing Escherichia coli (STEC) are regularly implicated in foodborne illness outbreaks and recalls of ground beef. In this study we determined the High Pressure Processing (HPP) D10 value (the processing conditions needed to reduce the microbial population by 1 log) of 39 STEC isolates, including the "b...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2015.07.001
更新日期:2015-12-01 00:00:00
abstract::Hong Qu, which mainly contains Monascus sp. and other microorganisms, as well as numerous microbial metabolites, is used as the fermentation starter of Hong Qu glutinous rice wine, a traditional alcoholic beverage. Two widely-used types of Hong Qu, namely Wuyi Qu (WYQ) and Gutian Qu (GTQ), were thoroughly compared for...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2017.07.019
更新日期:2018-02-01 00:00:00
abstract::A modular process risk model has been constructed that describes the manufacture of dairy dessert products and hazards that arise from non-proteolytic Clostridium botulinum. The model describes batch manufacture and consumer storage of a family size generic dairy dessert but includes a realistic quantification that co...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2010.04.004
更新日期:2011-04-01 00:00:00
abstract::During the production of sparkling wine, wine yeasts are subjected to many stress factors apart from ethanol, which lead to the need to achieve their acclimation in line with various industrial protocols. In the present work, 44 commercial wine Saccharomyces cerevisiae strains and one laboratory strain (BY4742) were f...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2014.12.006
更新日期:2015-06-01 00:00:00
abstract::The objective of this study was the development of DNA and RNA real-time PCR methods for detection of food-borne Salmonella sp. as rapid alternatives to the traditional cultural method (ISO 6579, 2004) in fresh meat carcasses and processed meat samples. These PCR methods were based on the hilA sequence, with primers a...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2010.10.012
更新日期:2011-05-01 00:00:00
abstract::This study evaluated whether the pre-exposure (24, 48 and 72 h) to sublethal conditions caused by acetic acid (AA), lactic acid (LA), sodium chloride (NaCl) or potassium chloride (KCl) could induce increased cross-tolerance to the essential oils from Origanum vulgare L. (OVEO) and Rosmarinus officinalis L. (ROEO) in d...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2019.103240
更新日期:2019-12-01 00:00:00
abstract::The current study reports a) the in situ transcriptional profiles of Listeria monocytogenes in response to fermented sausage stress and b) an approach in which in situ RT-qPCR data have been combined with advanced statistical techniques to discover potential stress resistance or cell viability biomarkers. Gene express...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2014.09.003
更新日期:2015-04-01 00:00:00
abstract::Kumis is a traditional fermented cow milk produced and consumed in South West Colombia. The main objective of this research was to studied the enterococcal population, present in 13 kumis samples traditionally manufactured, for their role as beneficial organisms or opportunistic pathogens. The molecular identification...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2011.02.006
更新日期:2011-08-01 00:00:00
abstract::The aim of this study was to target three genes of Staphylococcus aureus-fmhA (coding for a factor of unknown function), catalase and femA (coding for a factor essential for methicillin resistance) to establish and validate a PCR assay for the detection of this pathogen. Two pairs of primers were designed for fmhA and...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2008.01.010
更新日期:2008-05-01 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen emerging in hospitals as well as community and livestock. MRSA is a significant and costly public health concern because it may enter the human food chain and contaminate milk and dairy products causing foodborne illness. This study aimed to determine th...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2016.10.020
更新日期:2017-04-01 00:00:00
abstract::The occurrence of Listeria spp. and Listeria monocytogenes in retail RTE meat and fish products in Vancouver, British Columbia (B.C.) was investigated. To assess potential consumer health risk, recovered L. monocytogenes isolates were subjected to genotypic and phenotypic characterization. Conventional methods were us...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2011.12.015
更新日期:2012-06-01 00:00:00
abstract::The Traditional Mountain Malga (TMM) cheese is made from raw cow's milk by spontaneously fermentation in small farms called "Malga" located in Trentino region. This study was designed to characterize the lactic acid bacteria (LAB) growing on MRS medium, of TMM-cheese at the end of the ripening. Ninety-five LAB were is...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2014.12.003
更新日期:2015-06-01 00:00:00
abstract::Fourier transform infrared spectroscopy (FT-IR) can discriminate Escherichia coli O157:H7 ATCC 35150 from other bacteria: E. coli ATCC 25522, Bacillus cereus ATCC 10876, and Listeria innocua ATCC 51742 inoculated in to apple juice. Spectra of bacterial suspensions (ca. 10(9) cfu/ml in 0.9% NaCl) on Anodisc (aluminum o...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2005.01.017
更新日期:2006-04-01 00:00:00
abstract::The presence of verotoxin-producing Escherichia coli (VTEC) on bovine (n = 330) and pig (n = 120) carcasses in Poland was investigated using the ISO/TS 13136 standard. A total of 115 (34.8%) and 37 (30.8%) cattle and pig samples were positive in real-time PCR, respectively. Isolation of the bacteria revealed that from...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2020.103587
更新日期:2020-12-01 00:00:00
abstract::Today, edible insects represent a hot topic as an emerging and eco-friendly source of protein. The mealworm (Tenebrio molitor L.) is among the most employed insects for human consumption and feed purposes. So far Listeria monocytogenes, have never been detected either in products sold on the market or during the reari...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2019.05.006
更新日期:2019-10-01 00:00:00
abstract::The objectives of this study were to evaluate the growth and survival of the model probiotic strain Lactobacillus plantarum WCFS1 in co-culture with traditional yoghurt starters and to investigate the impact of preculturing on their survival and metabolite formation in set-yoghurt. L. plantarum WCFS1 was precultured u...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2016.04.008
更新日期:2016-10-01 00:00:00
abstract::Identification of Cronobacter represent a major challenge for laboratories testing powdered infant formula (PIF). In the present study, two biochemical galleries and three molecular methods have been applied to confirm 276 Cronobacter spp. and non-Cronobacter isolates from different sources. Using the latest database ...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2018.05.008
更新日期:2018-12-01 00:00:00
abstract::Mawè is a West African spontaneous fermented cereal-based dough. Different types of mawè exist varying in type of cereal and/or production condition, with fermentations lasting 24-48 h. With the aim of obtaining a comprehensive understanding of the microbial ecology of mawè processing, a microbiological characterisati...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2018.06.005
更新日期:2018-12-01 00:00:00
abstract::Following the detection of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in food-producing animals, both livestock and wildlife, and derived products, are considered potential sources of MRSA in humans. There is a paucity of data on MRSA in foods in Italy, and the data regarding wild animals are particularl...
journal_title:Food microbiology
pub_type: 杂志文章
doi:10.1016/j.fm.2015.07.012
更新日期:2015-12-01 00:00:00