Abstract:
:Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here, we describe a method that meets this challenge. The method entailed an optimized immunoprecipitation protocol coupled with two-dimensional chromatography and mass spectrometry. The ability to detect and quantify minute amounts of predefined antigens should be useful both for basic research in tumor immunology and for the development of rationally designed cancer vaccines.
journal_name
Cancer Immunol Resjournal_title
Cancer immunology researchauthors
Wang Q,Douglass J,Hwang MS,Hsiue EH,Mog BJ,Zhang M,Papadopoulos N,Kinzler KW,Zhou S,Vogelstein Bdoi
10.1158/2326-6066.CIR-19-0107subject
Has Abstractpub_date
2019-11-01 00:00:00pages
1748-1754issue
11eissn
2326-6066issn
2326-6074pii
2326-6066.CIR-19-0107journal_volume
7pub_type
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