Abstract:
:Quercetin is a flavonol polyphenol widely found in many vegetables, grains, and fruits. Quercetin has been shown to inhibit proliferation and invasion of various glioma cells and is regarded as a potential anticancer agent against glioma. However, whether and how this drug could affect brain blood vessels and endothelial cells (EC) are less understood. Further, there is hitherto no report on how quercetin affects brain EC Ca2+ homeostasis. In this report, we investigated the effects of quercetin on Ca2+ homeostasis in mouse brain bEnd.3 EC. We demonstrated that quercetin raised cytosolic Ca2+ level in a concentration-dependent manner. Quercetin-triggered Ca2+ signal composed of both internal Ca2+ release and extracellular Ca2+ influx. Quercetin caused Ca2+ release from the endoplasmic reticulum, and consistently, inhibition of inositol 1,4,5-trisphosphate receptor (IP3R) by xestospongin C (XeC) suppressed quercetin-triggered Ca2+ release. Quercetin also caused Ca2+ release from lysosomes, an observation in concordance with the inhibition of quercetin-triggered Ca2+ release by trans-Ned-19, a blocker of two-pore channels. As quercetin depleted intracellular Ca2+ storage, it suppressed ATP-induced Ca2+ release and thereby blunted ATP-triggered Ca2+ signaling. In addition, quercetin co-treatment significantly suppressed ATP-stimulated nitric oxide release. Our work therefore showed, for the first time, quercetin perturbed intracellular Ca2+ stores and strongly suppressed ATP-triggered response in bEnd.3 cells.
journal_name
Fundam Clin Pharmacoljournal_title
Fundamental & clinical pharmacologyauthors
Chen CY,Hour MJ,Shiao LR,Wong KL,Leung YM,Chan P,So ECdoi
10.1111/fcp.12514subject
Has Abstractpub_date
2020-04-01 00:00:00pages
213-221issue
2eissn
0767-3981issn
1472-8206journal_volume
34pub_type
杂志文章abstract::Clinical and experimental studies have shown that gender differences exist in cardiac repolarization in various animal species and human, as is evidenced by significantly longer QT, JT intervals and action potential duration in females than in males due to a reduced repolarization reserve in females. The latter is sho...
journal_title:Fundamental & clinical pharmacology
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doi:10.1111/j.1472-8206.2005.00384.x
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abstract::Intracerebroventricular (i.c.v.) injections of dihydropyridine derivatives calcium channel agonist (BAY K8644) and antagonist (nifedipine, nicardipine, PN 200-110) induced opposite long-lasting changes in blood pressure (BP) in pentobarbital anesthetized spontaneously hypertensive rats (SMR). I.c.v. nifedipine (NIF), ...
journal_title:Fundamental & clinical pharmacology
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doi:10.1111/j.1472-8206.1989.tb00474.x
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journal_title:Fundamental & clinical pharmacology
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journal_title:Fundamental & clinical pharmacology
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journal_title:Fundamental & clinical pharmacology
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doi:10.1111/j.1472-8206.1991.tb00700.x
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章
doi:10.1111/j.1472-8206.1996.tb00305.x
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章
doi:10.1111/fcp.12038
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journal_title:Fundamental & clinical pharmacology
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章,评审
doi:10.1111/j.1472-8206.1990.tb00041.x
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章
doi:10.1111/j.1472-8206.1989.tb00670.x
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章
doi:10.1111/j.1472-8206.1990.tb01011.x
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pub_type: 杂志文章,评审
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章
doi:10.1111/j.1472-8206.1995.tb00537.x
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journal_title:Fundamental & clinical pharmacology
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journal_title:Fundamental & clinical pharmacology
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journal_title:Fundamental & clinical pharmacology
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Fundamental & clinical pharmacology
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章,评审
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更新日期:1994-01-01 00:00:00
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journal_title:Fundamental & clinical pharmacology
pub_type: 杂志文章
doi:10.1111/j.1472-8206.2005.00391.x
更新日期:2006-02-01 00:00:00
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journal_title:Fundamental & clinical pharmacology
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