Abstract：
OBJECTIVE:Cyclin-dependent kinase 7 (CDK7) engages tumor growth by acting as a direct link between the regulation of transcription and the cell cycle. Here, we investigated the clinical significance of CDK7 expression and its potential as a therapeutic target in epithelial ovarian cancer (EOC). METHODS:CDK7 expression was examined in 436 ovarian tissues including normal to metastatic ovarian tumors using immunohistochemistry, and its clinical implications were analyzed. Furthermore, we performed in vitro and in vivo experiments using CDK7 siRNA or a covalent CDK7 inhibitor (THZ1) to elucidate the effect of CDK7 inhibition on tumorigenesis in EOC cells. RESULTS:The patient incidence of high CDK7 expression (CDK7High) gradually increased from normal ovarian epithelium to EOC (P < 0.001). Moreover, CDK7High was associated with an advanced stage and high-grade histology (P = 0.035 and P = 0.011, respectively) in EOC patients and had an independent prognostic significance in EOC recurrence (P = 0.034). CDK7 inhibition with siRNA or THZ1 decreased cell proliferation and migration, and increased apoptosis in EOC cells, and this anti-cancer mechanism is caused by G0/G1 cell cycle arrest. In in vivo therapeutic experiments using cell-line xenograft and PDX models, CDK7 inhibition significantly decreased the tumor weight, which was mediated by cell proliferation and apoptosis. CONCLUSION:Mechanistic interrogation of CDK7 revealed that it is significantly associated with an aggressive phenotype of EOC, and it has independent prognostic power for EOC recurrence. Furthermore, CDK7 may be a potential therapeutic target for patients with EOC, whether platinum sensitive or resistant.
journal_name
Gynecol Oncoljournal_title
Gynecologic oncologyauthors
Kim J,Cho YJ,Ryu JY,Hwang I,Han HD,Ahn HJ,Kim WY,Cho H,Chung JY,Hewitt SM,Kim JH,Kim BG,Bae DS,Choi CH,Lee JWdoi
10.1016/j.ygyno.2019.11.004subject
Has Abstractpub_date
2020-01-01 00:00:00pages
211-221issue
1eissn
0090-8258issn
1095-6859pii
S0090-8258(19)31625-7journal_volume
156pub_type
杂志文章abstract：:The oncogene specific mRNA of c-erbB-2 was detected by the S1 nuclease protection assay in 95 ovarian cancer specimens. In 79 primary carcinomas, we found 16 (20%) with strong expression, 13 (17%) with weak expression, 4 (5%) with very weak expression, and 46 (58%) with no expression. In 3 of 16 recurrencies (19%) a s...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1996.0226
更新日期：1996-08-01 00:00:00
abstract：OBJECTIVE:To examine the influence of diabetes and metformin therapy on overall survival (OS) and progression-free survival (PFS) in patients with endometrial cancer (EC) by using propensity score (PS) matching to account for confounding factors. METHODS:We retrospectively identified consecutive patients with stage I-...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2015.11.019
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVES:The aim of this study is to determine the impact of obesity on surgical and oncologic outcomes after primary debulking surgery (PDS) in advanced epithelial ovarian cancer (EOC). METHODS:Women with stage IIIC/IV EOC who underwent PDS with curative intent between 1/2/2003 and 12/30/2011 were included. Patient...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2014.07.103
更新日期：2014-10-01 00:00:00
abstract：:The common epithelial tumors of the human ovary have rarely been found in the gonads of intersex patients with gonadal dysgenesis or true hermaphroditism. This report describes a patient with ovarian serous cystadenocarcinoma and mixed gonadal dysgenesis (45,X/46,XY) and reviews other reported cases. Intersex patients...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(86)90306-9
更新日期：1986-07-01 00:00:00
abstract：:Retrospective review of sections of ovary from 50 patients with stage I, grade 1-3, epithelial ovarian carcinoma was performed to assess presence of cellular and nuclear atypia in noncancerous tissue adjacent to the primary tumor; ovarian tissue from 50 patients undergoing incidental oophorectomy was reviewed as well....
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(90)90075-v
更新日期：1990-09-01 00:00:00
abstract：:To determine the effects of neoadjuvant chemotherapy (NAC) in the management of cervical carcinoma Stage IB2 (tumor diameter > 4 cm), we reviewed 52 surgically treated patients diagnosed between January 1987 and December 1993. There were 20 patients treated with preoperative neoadjuvant chemotherapy and 32 treated by ...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1006/gyno.1997.4645
更新日期：1997-05-01 00:00:00
abstract：OBJECTIVE:The purpose of the present study is to prospectively evaluate the effects of tamoxifen on the pathological behavior of endometrial hyperplasias without atypia, diagnosed before the start of adjuvant endocrine therapy, in menopausal patients suffering from breast cancer. METHODS:Twenty-six patients suffering ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.10.010
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVE:To evaluate safety, tolerability, and antitumor activity of pembrolizumab monotherapy in patients with programmed death ligand 1 (PD-L1)-expressing advanced ovarian cancer enrolled in the multicohort, phase Ib KEYNOTE-028 trial. METHODS:Key inclusion criteria were age ≥18 years; advanced ovarian epithelial, ...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ygyno.2018.11.017
更新日期：2019-02-01 00:00:00
abstract：OBJECTIVE:To analyze the survival of women with malignant, mixed mullerian tumors of the ovary (OMMMT) compared to women with epithelial ovarian cancer (EOC). METHODS:Data from the Surveillance, Epidemiology and End Results (SEER) Program on 14025 women diagnosed with primary invasive ovarian cancer between 1988 and 1...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.02.016
更新日期：2004-05-01 00:00:00
abstract：:As the medical need and expenditure for chronic wound care have increased markedly over the past decade, wound centers have grown exponentially throughout the country. They can be community-based or hospital-based, and in either case, can be run by the facility or by a national chain. The wound center's viability is d...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2008.07.044
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVES:Data are lacking on how women view alternative approaches to surveillance for cervical cancer after treatment of high-grade cervical intraepithelial neoplasia. We measured and compared patient preferences (utilities) for scenarios with varying surveillance strategies and outcomes to inform guidelines and cos...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2010.05.002
更新日期：2010-08-01 00:00:00
abstract：:The presence of oncogenic HPV DNAs (HPV-16/18) in cervical carcinomas and their normal and metastatic pelvic lymph nodes and the expression patterns of proliferating cell nuclear antigen (PCNA) in cervical carcinomas were retrospectively studied to elucidate the possible roles of them in malignant transformation and p...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1996.0066
更新日期：1996-03-01 00:00:00
abstract：OBJECTIVE:To evaluate the hospital-acquired condition (HAC) following oophorectomy and/or hysterectomy for gynecologic cancer patients based on clinical outcomes and costs. MATERIALS AND METHODS:Data were obtained from the Nationwide Inpatient Sample from 2005 to 2011. Chi-squared and Wilcoxon rank sum two-sample test...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.07.009
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVES:Intraperitoneal (IP) chemotherapy following neoadjuvant chemotherapy (NACT) and interval tumor reductive surgery (TRS) for advanced ovarian cancer is feasible, however, the impact on disease outcomes remains unclear. We compare outcomes of patients treated with IP chemotherapy versus intravenous (IV) chemoth...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2019.12.016
更新日期：2020-03-01 00:00:00
abstract：:Human papillomavirus (HPV) types 16 and 18 are associated with cervical dysplasia and carcinoma. In vitro integration of HPV-16 or HPV-18 DNA into cultured human cervical cells results in their immortalization. In this study, in vivo differentiation of human cervical cells immortalized with recombinant HPV-16 or HPV-1...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(90)90082-v
更新日期：1990-09-01 00:00:00
abstract：:Gastrointestinal stromal tumors (GISTs) are c-kit-positive tumors that may arise anywhere in the tubular gastrointestinal tract. Around 5% of the cases arise elsewhere in the abdominal cavity. Tumors originating in the omentum and mesentery have been reported.A 31-year-old woman presented with pelvic pain, a palpable ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2003.08.025
更新日期：2003-12-01 00:00:00
abstract：OBJECTIVE:Sialylation involving tumor formation and invasive behavior goes along with altered sialyltransferase (ST) activity. A potent ST inhibitor, soyasaponin I (SsaI), was discovered to selectively inhibit the cellular alpha2,3-sialyltranserase activity. In this study, we further test the effects of SsaI on modifyi...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.10.010
更新日期：2005-02-01 00:00:00
abstract：:Twenty-six drug-resistant choriocarcinoma patients were treated with a PEBA regimen (cisplatin, etoposide, bleomycin, and adriamycin), supplemented by radiotherapy or surgery, from January 1988 to June 1992. A total of 80.8% of the patients had received at least four drugs, and 69.2% had received six or more cycles of...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1995.1037
更新日期：1995-02-01 00:00:00
abstract：OBJECTIVE:Compare detection of Lynch syndrome in endometrial cancer between regions of a health care system with different screening strategies. METHODS:A retrospective study of endometrial cancer (EC) cases from 2 regions of an integrated health care system (Kaiser Permanente Northern (KPNC) and Southern (KPSC) Calif...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2020.04.692
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:Malignant struma ovarii is a rare tumor, consisting of a struma ovarii with malignant change. Because of the rarity of the tumor, only a few reports with detailed data of thyroid function of the patient have been published. CASE:Medical and surgical treatments were performed in a case of malignant struma ov...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.2000.5966
更新日期：2000-12-01 00:00:00
abstract：:Current first-line management for advanced ovarian cancer consists of cytoreductive surgery followed by chemotherapy, usually with a platinum/taxane combination. Although this approach has been shown to achieve overall response rates of 70-80% in clinical trials, the majority of patients relapse. A number of different...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/s0090-8258(03)00472-4
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:Comorbidities are known to increase endometrial cancer risk, but the separate and combined impact of these risk factors on endometrial cancer survival remains unclear. This study aimed to determine the associations between metabolic syndrome and its components with disease-free survival, overall survival, end...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2020.06.488
更新日期：2020-09-01 00:00:00
abstract：:Over a 7-year period from 1987 to 1994, 120 patients consecutive patients with FIGO stage IB invasive cervical cancer who underwent type 3 radical abdominal hysterectomy and bilateral pelvic lymphadenectomy had either Jackson-Pratt closed-suction drainage (Group 1, patients 1-60) or no drainage (Group 2, patients 61-1...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1006/gyno.1995.1131
更新日期：1995-05-01 00:00:00
abstract：:To determine the morbidity associated with delivery of intraperitoneal chemotherapy via subcutaneous semipermanent infusion catheters, we analyzed the data on 227 patients treated from April 1985 through December 1989. A total of 249 catheters were inserted and used to administer chemotherapy in these patients. There ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(91)90266-8
更新日期：1991-05-01 00:00:00
abstract：OBJECTIVE:To assess trends in guideline-adherent fertility-sparing surgery (GA-FSS) for early-stage cervical cancer relative to Patient Protection and Affordable Care Act (ACA) implementation. METHODS:National Cancer Database patients treated for Stage IA1-IB1 cervical cancer from 2004 to 2016 were included. Multivari...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2020.05.027
更新日期：2020-08-01 00:00:00
abstract：OBJECTIVES:We sought to determine a baseline and five-year follow up sexual function score in women undergoing hysterectomy for endometrial cancer. METHODS:A cross-section of endometrial cancer patients receiving care from 2006 to 2010 was identified. Patients were surveyed during academic year 2011 using the Pelvic O...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.10.025
更新日期：2019-01-01 00:00:00
abstract：:Ascitic fluid was obtained from a patient with ovarian endometrioid carcinoma. The cells obtained from the fluid were cultured in Eagle's minimum essential medium containing 15% fetal calf serum at 37 degrees C under humidified 5% CO2 and 95% air. Epithelial colonies grew rapidly in the primary culture and we were abl...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(84)90217-8
更新日期：1984-03-01 00:00:00
abstract：:A simple technique of closing the defect following simple vulvectomy is described. The labia minora as a pedicle graft avoids the problems encountered by conventional methods. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(74)90058-4
更新日期：1974-12-01 00:00:00
abstract：OBJECTIVE:This study investigated the relationship between 13 proteins involved in DNA damage and the outcomes of patients with recurrent ovarian cancer (ROC). PATIENTS AND METHODS:Immunohistochemistry staining was performed in 114 diagnostic samples from patients with serous ROC who participated in the OVA-301 study,...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2013.10.032
更新日期：2014-01-01 00:00:00
abstract：STUDY OBJECTIVE:To describe our technique for excision of the pre-caval and laterocaval nodes using an extraperitoneal approach. This technique was developed to make the dissection and excision of the less accessible nodes in an easier and safer way by minimizing the risk of great vessels injury and bleeding. DESIGN:S...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2017.10.030
更新日期：2018-01-01 00:00:00
