Spermine and spermidine, modulators of the cell surface enzyme adenylate cyclase.

Abstract:

:Adenylate cyclase activity was measured in membrane preparations of cultured fibroblasts from controls and patients with cystic fibrosis. Enzyme activity increased as the transition from exponential growth to confluence occurred; sodium fluoride-stimulated activity more markedly displayed this relationship than basal cyclase activity. The in vitro addition of spermine (1 X 10(-6) to 2 X 10(-3) M) to membrane preparations caused inhibition of basal and sodium fluoride-stimulated enzyme activity, with 50% inhibition of basal activity occurring at 10(-6) M spermine. Spermidine (10(-4) M) caused 15--25% inhibition of adenylate cyclase activity. The increase in fibroblast adenylate cyclase activity during the transition from exponential growth was comparable in cells obtained from cystic fibrosis patients and control subjects. Basal and sodium-fluoride stimulated adenylate cyclase activity as well as inhibition of this enzyme activity by spermidine and spermine were undistinguishable between the different cell genotypes. A potential modulation of cellular proliferative activity through polyamine interaction with the adenylate cyclase system is postulated.

journal_name

Pediatr Res

journal_title

Pediatric research

authors

Wright RK,Buehler BA,Schott SN,Rennert OM

doi

10.1203/00006450-197808000-00005

subject

Has Abstract

pub_date

1978-08-01 00:00:00

pages

830-3

issue

8

eissn

0031-3998

issn

1530-0447

journal_volume

12

pub_type

杂志文章
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