当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Kinetic model of Chlorella vulgaris growth with and without extremely low frequency-electromagnetic fields (EM-ELF).

Kinetic model of Chlorella vulgaris growth with and without extremely low frequency-electromagnetic fields (EM-ELF).

Abstract：

:Chlorella vulgaris was grown in two bench-scale photobioreactors with and without the application of a low intensity, low frequency electromagnetic field (EM-ELF) of about 3mT. Cell concentration and tendency of cells to form aggregates inside the reactor were recorded over a 30 days-time period at 0.5L-constant medium volume in the temperature range 289-304K. At 304K, after a cultivation period of 15 days, the rate of cell death became predominant over that of growth. In the temperature range 289-299K, a two step-kinetic model based on the mitotic division and the clusterization processes was developed and critically discussed. The best-fitted curves turned out to have a sigmoid shape, and the competition between mitosis and clusterization was investigated. Without EM-ELF, the temperature dependence of the specific rate constant of the mitotic step yielded an apparent total enthalpy of 15±6kJmol(-1), whose value was not influenced by the EM-ELF application. The electromagnetic field was shown to exert a significant effect on the exothermic clusterization step. The heat exchange due to binding between cells and liquid medium turned out to be -44±5kJmol(-1) in the absence of EM-ELF and -68±8kJmol(-1) when it was active. Optical microscopy observations were in agreement with the model predictions and confirmed that EM-ELF was able to enhance cell clusterization.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Beruto DT,Lagazzo A,Frumento D,Converti A

doi

10.1016/j.jbiotec.2013.10.035

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

9-14

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(13)00481-1

journal_volume

169

pub_type

杂志文章

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