Abstract:
PURPOSE:The purpose of the present study was to use the human colorectal carcinoma cell line. Caco-2, as a human intestinal epithelial model for studying the effects of cyclodextrins as absorption enhancers. METHODS:Cyclodextrins of varying sizes and physicochemical characters were investigated. The effects of the cyclodextrins were evaluated by means of staining of the cytoplasma and determination of the mitochondrial dehydrogenase activity as well as by transport enhancement of the macromolecular pore marker polyethylene glycol 4000 (PEG-4000) across the Caco-2 monolayers. RESULTS:The transport enhancing properties of the cyclodextrins were found to follow the lipophilicity of the core in their cyclic structure. Dimethyl-beta-cyclodextrin was the most powerful in all aspects and caused an increase in the permeability of the cytoplasma membrane in a concentration dependent manner. It was possible to increase the overall transport of PEG-4000 10-fold by the use of dimethyl-beta-cyclodextrin in low concentrations where the toxic effects on the monolayers were insignificant. It was further observed that the basolateral membrane was significantly more sensitive to cyclodextrins than the apical membrane. CONCLUSIONS:Since dimethyl-beta-cyclodextrin was able to produce an absorption enhancing effect on PEG-4000 in concentrations where the toxic effects on Caco-2 monolayers were low it is worth to pursue the compound as an absorption enhancer.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Hovgaard L,Brøndsted Hdoi
10.1023/a:1016225707807subject
Has Abstractpub_date
1995-09-01 00:00:00pages
1328-32issue
9eissn
0724-8741issn
1573-904Xjournal_volume
12pub_type
杂志文章abstract:PURPOSE:To show, using a model study, how electronic structure theory can be applied in combination with LC/UV/MS/MS for the prediction and identification of oxidative degradants. METHODS:The benzyloxazole 1, was used to represent an active pharmaceutical ingredient for oxidative forced degradation studies. Bond disso...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/b:pham.0000041469.96466.12
更新日期:2004-09-01 00:00:00
abstract:PURPOSE:Herein we report the discovery of a group of derivatized alpha-amino acids that increase the oral bioavailability of sodium cromolyn. METHODS:We prepared three N-acylated alpha-amino acids and used these compounds to demonstrate the oral delivery of cromolyn in an in vivo rat model. In vitro experiments, inclu...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016034913181
更新日期:1996-02-01 00:00:00
abstract:PURPOSE:HSP90 (Heat shock protein 90kD) has been validated as a therapeutic target in Castrate Resistant Prostate Cancer. Unfortunately, HSP90 inhibitors suffer from dose-limiting toxicities that hinder their clinical applications. Previously developed polymeric delivery systems for HSP90 inhibitors had either low drug...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-017-2249-5
更新日期:2017-12-01 00:00:00
abstract::A quantitative analysis of the dependence of dissolution rate on the relative surface area occupied by two non-interacting drug mixtures from co-compressed slabs is described. The results from the experimental dissolution rates of each component from naproxen/phenytoin co-compressed slabs under laminar flow conditions...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1018979419714
更新日期:1994-10-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate the specific changes in body distribution of camptothecin (CA) through incorporation into solid lipid nanoparticles (SLN) by peroral route. METHODS:Camptothecin loaded solid lipid nanoparticles (CA-SLN) coated with poloxamer 188 were produced by high pressure homogeniza...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1018888927852
更新日期:1999-05-01 00:00:00
abstract:PURPOSE:To prepare acylated exenatide analogues and investigate their biological properties for guiding the development of PLGA formulations of exenatide. METHODS:The acylated exenatide analogues were prepared by reaction with glycolic acid (GA), one constitutional unit of PLGA, and characterized by HPLC-MS/MS and Cir...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-014-1298-2
更新日期:2014-08-01 00:00:00
abstract:PURPOSE:The cell surface adhesion molecule CD44 plays important roles in the initiation and development of atherosclerotic plaques. We aim to develop nanoparticles that can selectively target CD44 for the non-invasive detection of atherosclerotic plaques by magnetic resonance imaging. METHODS:Magnetic glyconanoparticl...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-013-1021-8
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:A series of studies was conducted to evaluate the preclinical pharmacokinetics of SB-239063 (trans-1-(4-hydroxycyclohexyl)-4-(4-fluorophenyl)-5-[(2-methoxy)pyrimidin-4-yl] imidazole), a potent and selective p38 MAP kinase inhibitor. METHODS:SB-239063 was administered both i.v. and p.o. in the rat, dog, cynomol...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1013002414678
更新日期:2001-09-01 00:00:00
abstract::The permeation enhancing property of 5% oleic acid in ethanol on beta-estradiol was investigated in vitro and in vivo using symmetrical and asymmetrical side-by-side diffusion cells and the human skin sandwich flap, respectively. beta-Estradiol permeability in vitro and in vivo was similar in 75% ethanol (ETOH). Oleic...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1018974131236
更新日期:1993-12-01 00:00:00
abstract::The mechanism of release from sustained-release adinazolam mesylate tablets was assessed by the Higuchi equation and by analysis of drug release profiles through 60% released using the Peppas equation. Computed values of the diffusional exponent, n, ranged from 0.59 to 0.66. Values of n in this range are consistent wi...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015834114359
更新日期:1991-12-01 00:00:00
abstract:PURPOSE:Several phenomena in tablets indicate that an inhomogeneous pore distribution is formed during the compaction process. Examples are lamination and the capping of corners. In order to gain an understanding of the relation between structure and compact properties, analyzing the structure in a location dependent m...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-005-5280-x
更新日期:2005-08-01 00:00:00
abstract::We describe a simplified and rapid liquid chromatographic determination of antipyrine clearance (CLAP) calculated from peak height ratios of drug/internal standard. Saliva or plasma was collected 24 hr after the oral administration of 1 g of antipyrine to the subject. A 25-microliter aliquot of the sample is deprotein...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015816825920
更新日期:1991-02-01 00:00:00
abstract:PURPOSE:The purpose of this study was to determine the potential effect of probenecid on the pharmacokinetics of zalcitabine in HIV-positive patients. METHODS:Twelve patients received single oral 1.5 mg doses of zalcitabine alone and during probenecid treatment (500 mg at 8 and 2 hours before and 4 hours after zalcita...
journal_title:Pharmaceutical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1023/a:1016009029536
更新日期:1996-03-01 00:00:00
abstract:PURPOSE:Information on genetic variants that affect the pharmacokinetics and pharmacodynamics (PK/PD) of drugs in different populations from Mexico is still an ongoing endeavor. Here, we investigate allele frequencies on pharmacogenetic targets in Mexican Mestizos and Natives from three different States and its associa...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-016-1990-5
更新日期:2016-11-01 00:00:00
abstract:PURPOSE:A rapid immune response is required to prevent death from Anthrax, caused by Bacillus anthracis. METHOD:We formulated a vaccine carrier comprised of acetalated dextran microparticles encapsulating recombinant protective antigen (rPA) and resiquimod (a toll-like receptor 7/8 agonist). RESULTS:We were able to p...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-013-0975-x
更新日期:2013-05-01 00:00:00
abstract:PURPOSE:(i) To use trehalose as a model compound to evaluate the concept of crystallinity in pharmaceuticals. (ii) To understand the structural nature of dehydrated trehalose dihydrate. MATERIALS AND METHODS:Trehalose dihydrate was dehydrated isothermally at several temperatures below 100 degrees C and the anhydrous p...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-006-9058-6
更新日期:2006-10-01 00:00:00
abstract:PURPOSE:The aim of this study is to investigate the effects of 50% ethyl acetate extracts of grapefruit juice (GFJ) and orange juice (OJ) on the transport activity of P-glycoprotein (P-gp) in the rat small intestine. METHODS:The efflux of P-gp substrates from rat everted sac in the absence or presence of verapamil, GF...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016100715125
更新日期:2002-06-01 00:00:00
abstract:PURPOSE:The sensitivity and effectiveness of indirect metrics proposed for the assessment of comparative absorption rates in bioequivalence studies [Cmax, Tmax, partial AUC (AUCp), feathered slope (SLf), intercept metric (I)] were originally tested by assuming first-order absorption. The present study re-evaluates thei...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1007521016985
更新日期:2000-05-01 00:00:00
abstract:PURPOSE:The intratumoral heterogeneity observed in breast cancer (BC), in particular with regard to cell surface receptor expression, can hinder the success of many targeted cancer therapies. The development of novel therapeutic agents that target more than one receptor can overcome this inherent property of tumors and...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-019-2684-6
更新日期:2020-01-21 00:00:00
abstract::A new method of analysis for the antihypertensive drug, hydralazine, is introduced. The assay involves the addition of p-nitrobenzaldehyde to blood samples containing hydralazine, to form a stable Schiff's base, hydralazine p-nitrobenzaldehyde hydrazone. The derivative is extracted from the blood into hexane and the s...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015915812987
更新日期:1988-06-01 00:00:00
abstract:PURPOSE:This study aims to conduct an impact investigation in the hydrophobic-hydrophilic balance as an important factor for dissolution improvement of a hydrophilic carrier-based solid dispersion system. METHODS:Polymeric carriers with different hydrophobic to hydrophilic ratios were used to prepare several electrosp...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-019-2734-0
更新日期:2020-01-07 00:00:00
abstract:PURPOSE:Knowledge about the uptake mechanism and subsequent intracellular routing of non-viral gene delivery systems is important for the development of more efficient carriers. In this study we compared two established cationic polymers pDMAEMA and PEI with regard to their transfection efficiency and mechanism of cell...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-007-9287-3
更新日期:2007-08-01 00:00:00
abstract:PURPOSE:Previous literature has suggested the absence of an effective barrier between the nasal mucosa and the brain for compounds administered via the nasal route. These experiments were conducted to elucidate the role of the blood-brain barrier efflux transporter P-glycoprotein (P-gp) in attenuating delivery of P-gp ...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1025053115583
更新日期:2003-08-01 00:00:00
abstract::Liver fibrosis is a pathological condition originating from liver damage that leads to excess accumulation of extracellular matrix (ECM) proteins in the liver. Viral infection, chronic injury, local inflammatory responses and oxidative stress are the major factors contributing to the onset and progression of liver fib...
journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
doi:10.1007/s11095-014-1497-x
更新日期:2015-02-01 00:00:00
abstract:PURPOSE:The present study evaluates immobilized artificial membrane (IAM) chromatography for predicting drug permeability across the blood-brain barrier (BBB) and outlines the potential and limitations of IAMs as a predictive tool by comparison with conventional methods based on octanol/water partitioning and octadecyl...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1011904311149
更新日期:1998-08-01 00:00:00
abstract::To clarify the mechanism by which basic drugs accumulate in the lung, the binding selectivity of drugs to different subcellular structures of the perfused rat lung was examined. Imipramine, quinine, and metoclopramide were used as examples of basic drugs. The accumulation of basic drugs was highest in the mitochondria...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016481911538
更新日期:1987-02-01 00:00:00
abstract:PURPOSE:Effects of polyvinylpyrrolidone (PVP) molecular weight on the solid-state intermolecular interactions among probucol/PVP/sodium dodecyl sulfate (SDS) ternary ground mixtures (GM) and the formation of nanoparticles were investigated by solid-state NMR spectroscopy. MATERIALS AND METHODS:Ternary GMs of probucol ...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-006-9089-z
更新日期:2006-11-01 00:00:00
abstract:PURPOSE:Within-subject dependency of observations has a strong impact on the evaluation of population pharmacokinetic (PK) and/or pharmacodynamic (PD) models. To our knowledge, none of the current model evaluation tools correctly address this issue. We present a new method with a global test and easy diagnostic plot wh...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-011-0422-9
更新日期:2011-08-01 00:00:00
abstract::Nanobiosensors with high sensitivity and specificity have shown great potential in the detection of diseases. The incorporation of therapeutic agents with nanobiosensors allows the simultaneous diagnosis and therapy of diseases. The delivery of nanobiosensors and therapeutic agents using polymers is a common strategy ...
journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
doi:10.1007/s11095-016-1944-y
更新日期:2016-10-01 00:00:00
abstract::The partitioning of a series of nine nitroimidazole drugs in liposomes (log Km) of various compositions has been determined and compared to their partitioning in the n-octanol/saline system (log K) at 30 degrees C. The log Km ranged from 1.5 to 0.5 and was three- to fourfold greater than the log K; further, the linear...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015931431817
更新日期:1989-05-01 00:00:00