Microcalorimetric study of metabolic inhibition by humic acids in mitochondria from Oryctolagus cuniculus domestica liver cells.

Abstract:

:The metabolic thermogenesis curves of mitochondria isolated from Oryctolagus cuniculus domestica liver tissue have been determined by using an LKB-2277 bioactivity monitor. After isolation, mitochondria maintain activity when stored in appropriate nutrients. The thermokinetic equation: [formula: see text] was established for the activity recovery phase of metabolism. For these studies, the order of metabolism was found to be zero. The metabolic activity of mitochondria was inhibited by humic acid, a potential toxicant in Kashin-Beck and Keshan diseases. Experimental results indicate that humic acid will effect the heat output and lag time of mitochondrial metabolism.

journal_name

Chemosphere

journal_title

Chemosphere

authors

Liu Y,Wang XQ,Xie CL,Qu SS,Deng FJ,Guo Y

doi

10.1016/0045-6535(96)00151-8

subject

Has Abstract

pub_date

1996-07-01 00:00:00

pages

99-105

issue

1

eissn

0045-6535

issn

1879-1298

pii

0045653596001518

journal_volume

33

pub_type

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